Tryptase alpha/beta 1 Antibody (AA1)

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Human tonsil stained with Mast Cell Tryptase antibody.

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Analysis of Mast Cell Tryptase formalin-fixed paraffin embedded human tonsil using NBP2-26444.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-Fr, IHC-P
• Clone: AA1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Tryptase alpha/beta 1 Antibody (AA1) Summary

• Immunogen: BALB/C mice were injected with a purified human lung tryptase.
• Localization: Cytoplasmic
• Specificity: Due to sequence identity this antibody will likely cross react with tryptase beta 2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPSAB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:300-1:600
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:300-1:600
  • Western Blot 1:100-1:2000
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Reactivity Notes

Rat and mouse reactivity reported in scientific literature. Please see Publications tab for full list. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Tryptase alpha/beta 1 Antibody (AA1)

• EC:3.4.21.59
• epididymis secretory sperm binding protein
• mast cell alpha II tryptase
• mast cell beta I tryptase
• mast cell tryptase
• TPS1
• TPS2
• TPSB1
• TPSB2
• tryptase alpha II
• Tryptase alpha-1
• tryptase beta I
• tryptase beta-1
• Tryptase beta-2
• Tryptase I
• tryptase II
• tryptase-1
• tryptase-2
• tryptase-I
• tryptase-III

Background

Principal Names: Tryptase alpha/beta 1 Official Gene Symbol: TPSAB1 Gene ID: 7177 (Human) Gene Map Locus: 16p13.3 (Human). Human tryptases are divided into two groups, alpha and beta. beta-Tryptase appears to be the major type stored in mast cell secretory granules and alpha-tryptase is the major isoform expressed by basophils.beta-tryptase, a major constituent of mast cell secretory granules, has emerged recently as the most specific marker in vivo of mast cell activation in humans. It is a trypsin like serine protease that hydrolyzes peptide bonds on the C-terminal side of basic amino acids. Unlike trypsin, however, it is highly selective in hydrolyzing its peptide and protein targets and resists inactivation by circulating inhibitors. It has potential roles in the lung, skin, and other tissues as a local anticoagulant, activator of collagenase, promoter of smooth muscle contraction, and modulator of neuropeptide activity. The pathogenic role for tryptases has been observed in asthmatic airway inflammation other inflammatory disorders. The gene is mapped on human chromosome 16p13.3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptase alpha/TPS1 Antibody (NBP2-26444)(12)

Publications using NBP2-26444

• Moriyama H, Endo J, Kataoka M et al. Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling Nature communications 2022-05-31 [PMID: 35641514] (IHC-P, Mouse)
• Cordner ZA, Li Q, Liu L et al. Vagal gut-brain signaling mediates amygdaloid plasticity, affect and pain in a functional dyspepsia model JCI insight 2021-02-16 [PMID: 33591956] (IHC)
• Maiese A, Scopetti M, Santurro A et al. Corpse dismemberment: A case series. Solving the puzzle through an integrated multidisciplinary approach J Forensic Leg Med 2020-10-05 [PMID: 33012308]
• Gao F, Fish BL, Szabo A et al. Enhanced survival from radiation pneumonitis by combined irradiation to the skin. Int. J. Radiat. Biol. 2014-05-14 [PMID: 24827855] (IHC-P, Rat)
• Farkas D, Kraskauskas D, Drake JI et al. CXCR4 inhibition ameliorates severe obliterative pulmonary hypertension and accumulation of C-kit(+) cells in rats. PLoS ONE. 2014-03-03 [PMID: 24587052] (IHC-P, Rat)
• Masaka T, Iijima K, Endo H et al. Gender differences in oesophageal mucosal injury in a reflux oesophagitis model of rats. Gut. 2013-01-01 [PMID: 22287598] (IHC-P, Rat)
  
  Details:
  Product cited: Mast Cell Tryptase (IMG-80250). IHC(paraffin): Normal and reflux esophagitis (RE) rat esophagus tissue: Fig S1.
• Le BV, Khorsi-Cauet H, Bach V, Gay-Queheillard J. Mast cells mediate Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation in rat. Eur J Clin Microbiol Infect Dis. 2012-08-01 [PMID: 22282020]
  
  Details:
  Products cited: Mast Cell Tryptase (IMG-80250).
• Shimada R, Nakao K, Furutani R, Kibayashi K. A rat model of changes in dural mast cells and brain histamine receptor H3 expression following traumatic brain injury. J Clin Neurosci. 2012-03-01 [PMID: 22277566] (IHC-P, Mouse)
  
  Details:
  Product Cited: Mast Cell Tryptase (IMG-80250). 1. IHC(paraffin): Mouse brain (Cerebral Cortex) with/out induced traumatic brain injury (TBI), Figure 1A & B.
• Sato Y, Harada K, Kizawa K et al. Activation of the MEK5/ERK5 cascade is responsible for biliary dysgenesis in a rat model of Caroli's disease. Am J Pathol. 2005-01-01 [PMID: 15631999] (IHC-P, Rat)
  
  Details:
  IHC (paraffin): rat liver, Fig 3B inset.
• Haase MG, Klawitter A, Baretton GB. IkappaBgamma is expressed in mast cells. Virchows Arch. 2004-11-01 [PMID: 15378359] (IHC-P, Rat)
  
  Details:
  IHC (paraffin): Fig 1 (rat lung tissue sections), Fig 2 (rat peritoneal macrophages).
• Akiyoshi H, Terada T. Mast cell, myofibroblast and nerve terminal complexes in carbon tetrachloride-induced cirrhotic rat livers. J Hepatol. 1998-07-01 [PMID: 9696499] (IHC-P, Rat)
  
  Details:
  IHC (paraffin): rat liver (data described in Results section, page 114).
• Walls AF, Jones DB, Williams JH et al. Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase. J Pathol. 1990-10-01 [PMID: 2250189] (IHC-P, WB, Human)
  
  Details:
  IHC (paraffin) in human: Fig 1 (skin), Fig 2 (lung), Fig 4 (A:bronchiole, B:lung alveoli, C:T-lymphocyte area of tonsil, D:septum of tonsil, E:colonic mucosa and F:skin), Table 1 (summary of staining in multiple tissues). Peripheral blood basophils were u

Bioinformatics

• Gene Symbol: TPSAB1