TRPV5 Antibody - BSA Free

Western Blot: TRPV5 Antibody - BSA Free [NBP2-94490] - Western blot analysis of various lysates, using TRPV5 Rabbit pAb (A6473) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.

Immunohistochemistry: TRPV5 Antibody - BSA Free [NBP2-94490] - Immunohistochemistry analysis of paraffin-embedded human kidney using TRPV5 Rabbit pAb (A6473) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRPV5 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 655-725 of human TRPV5 (NP_062815.3). DKEDDQEHPSEKQPSGAESGTLARASLALPTSSLSRTASQSSSHRGWEILRQNTLGHLNLGLNLSEGDGEE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPV5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TRPV5 Antibody - BSA Free

• Calcium transport protein 2
• calcium transporter 2
• CAT2
• ECaC
• ECAC1
• ECAC1CaT2
• Epithelial calcium channel 1CAT2
• Osm-9-like TRP channel 3
• OTRPC3
• transient receptor potential cation channel subfamily V member 5
• transient receptor potential cation channel, subfamily V, member 5
• TRPV5

Background

TRPV5 is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPV5