GIT1 Antibody (S39B-8)

Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.

Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X.

Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, KO
• Clone: S39B-8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GIT1 Antibody (S39B-8) Summary

• Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
• Localization: Cytoplasm
• Specificity: Detects approx 90kDa. Does not cross-react with GIT2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GIT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of GIT1 Antibody was sufficient for detection of GIT1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31908016). Use in KO reported in secitific publication PMID: 32460388

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30546041).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GIT1 Antibody (S39B-8)

• ARF GAP GIT1
• ARF GTPase-activating protein GIT1
• CAT1
• CAT-1
• Cool-associated and tyrosine-phosphorylated protein 1
• G protein-coupled receptor kinase interacting ArfGAP 1
• G protein-coupled receptor kinase interactor 1
• G protein-coupled receptor kinase-interactor 1
• GIT1
• GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GIT1 Antibody (NBP2-22423)(6)

Publications using NBP2-22423

• Schlienger, S;Yam, PT;Balekoglu, N;Ducuing, H;Michaud, JF;Makihara, S;Kramer, DK;Chen, B;Fasano, A;Berardelli, A;Hamdan, FF;Rouleau, GA;Srour, M;Charron, F; Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control Science advances 2023-05-12 [PMID: 37172092] (IHC-Fr, PLA, ICC/IF, WB, Mouse)
• Zhao SJ, Liu H, Chen J et al. Macrophage GIT1 contributes to bone regeneration by regulating inflammatory responses in an ERK/NRF2-dependent way J. Bone Miner. Res. 2020-05-27 [PMID: 32460388] (WB, KO, Mouse)
• Huang Y, Gu C, Wang Q, et al. The protective effort of GPCR kinase 2 interacting protein 1 in neurons via promoting Beclin1 Parkin induced mitophagy at the early stage of spinal cord ischemia reperfusion injury FASEB j. 2019-12-27 [PMID: 31908016] (ICC/IF, WB, IHC-F, Mouse)
• Li L, Tang P, Zhou Z et al. GIT1 regulates angiogenic factor secretion in bone marrow mesenchymal stem cells via NF-kappa B/Notch signalling to promote angiogenesis Cell Prolif. [PMID: 31502302] (IP)
• Zhao SJ, Kong FQ, Cai W et al. GIT1 contributes to autophagy in osteoclast through disruption of the binding of Beclin1 and Bcl2 under starvation condition Cell Death Dis 2018-12-13 [PMID: 30546041] (WB, Mouse)
• Chen J, Wang Q, Zhou W et al. GPCR kinase 2-interacting protein-1 protects against ischemia-reperfusion injury of the spinal cord by modulating ASK1/JNK/p38 signaling FASEB J. [PMID: 29912587]

Bioinformatics

• Gene Symbol: GIT1
• Uniprot:
  • Q9Z272(Rat)