Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary ...read more
Novus Biologicals Knockout (KO) Validated Mouse GIT1 Antibody (S39B-8) - BSA Free (NBP2-22423) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-GIT1 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Localization
Cytoplasm
Specificity
Detects approx 90kDa. Does not cross-react with GIT2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GIT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
1 ug/ml of GIT1 Antibody was sufficient for detection of GIT1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31908016). Use in KO reported in secitific publication PMID: 32460388
Publications
Read Publications using NBP2-22423 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 30546041).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified
Alternate Names for GIT1 Antibody (S39B-8) - BSA Free
ARF GAP GIT1
ARF GTPase-activating protein GIT1
CAT1
CAT-1
Cool-associated and tyrosine-phosphorylated protein 1
G protein-coupled receptor kinase interacting ArfGAP 1
G protein-coupled receptor kinase interactor 1
G protein-coupled receptor kinase-interactor 1
GIT1
GRK-interacting protein 1
Background
G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 9 applications: ICC/IF, IHC-F, IP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, KO, Proximity Ligation Assay, WB, Western Blot.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our GIT1 Antibody (S39B-8) - BSA Free and receive a gift card or discount.