GIT1 Antibody (S39B-8)

Images

 
Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 ...read more
Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP, KO
Clone
S39B-8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

GIT1 Antibody (S39B-8) Summary

Immunogen
Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Localization
Cytoplasm
Specificity
Detects approx 90kDa. Does not cross-react with GIT2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GIT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
Application Notes
1 ug/ml of GIT1 Antibody was sufficient for detection of GIT1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31908016). Use in KO reported in secitific publication PMID: 32460388
Control
GIT1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP2-22423 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • KO
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30546041).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GIT1 Antibody (S39B-8)

  • ARF GAP GIT1
  • ARF GTPase-activating protein GIT1
  • CAT1
  • CAT-1
  • Cool-associated and tyrosine-phosphorylated protein 1
  • G protein-coupled receptor kinase interacting ArfGAP 1
  • G protein-coupled receptor kinase interactor 1
  • G protein-coupled receptor kinase-interactor 1
  • GIT1
  • GRK-interacting protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GIT1 Antibody (NBP2-22423)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GIT1 Antibody (NBP2-22423) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-22423AF405
Alexa Fluor 488 NBP2-22423AF488
Alexa Fluor 647 NBP2-22423AF647
Alexa Fluor 700 NBP2-22423AF700
Biotin NBP2-22423B
DyLight 350 NBP2-22423UV
DyLight 405 NBP2-22423V
DyLight 488 NBP2-22423G
DyLight 550 NBP2-22423R
DyLight 650 NBP2-22423C
DyLight 680 NBP2-22423FR
DyLight 755 NBP2-22423IR
FITC NBP2-22423F
HRP NBP2-22423H
Janelia Fluor 549 NBP2-22423JF549
Janelia Fluor 646 NBP2-22423JF646

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Bioinformatics

Gene Symbol GIT1
Uniprot