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GIT1 Antibody (S39B-8)

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Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:1000.
Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 ...read more
Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP, KO
Clone
S39B-8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-22423AF405
Alexa Fluor 488
NBP2-22423AF488
Alexa Fluor 647
NBP2-22423AF647
Alexa Fluor 700
NBP2-22423AF700
Biotin
NBP2-22423B
DyLight 350
NBP2-22423UV
DyLight 405
NBP2-22423V
DyLight 488
NBP2-22423G
DyLight 550
NBP2-22423R
DyLight 650
NBP2-22423C
DyLight 680
NBP2-22423FR
DyLight 755
NBP2-22423IR
FITC
NBP2-22423F
HRP
NBP2-22423H
Janelia Fluor 549
NBP2-22423JF549
Janelia Fluor 646
NBP2-22423JF646
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GIT1 Antibody (S39B-8) Summary

Immunogen
Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Localization
Cytoplasm
Specificity
Detects approx 90kDa. Does not cross-react with GIT2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GIT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
Application Notes
1 ug/ml of GIT1 Antibody was sufficient for detection of GIT1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31908016). Use in KO reported in secitific publication PMID: 32460388
Control
GIT1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP2-22423 in the following applications:

  • ICC/IF
    1 publication
  • IHC-F
    1 publication
  • IP
    1 publication
  • KO
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30546041).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GIT1 Antibody (S39B-8)

  • ARF GAP GIT1
  • ARF GTPase-activating protein GIT1
  • CAT1
  • CAT-1
  • Cool-associated and tyrosine-phosphorylated protein 1
  • G protein-coupled receptor kinase interacting ArfGAP 1
  • G protein-coupled receptor kinase interactor 1
  • G protein-coupled receptor kinase-interactor 1
  • GIT1
  • GRK-interacting protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: GIT2 Antibody [NBP2-16661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GIT2 protein stained by GIT2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Frozen: GIT2 Antibody [NBP2-16661] - GIT2 antibody detects GIT2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: GIT2 protein stained by GIT2 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
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Western Blot: SLC7A2 Antibody [NBP2-93699] - Analysis of extracts of various cell lines, using SLC7A2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: SLC7A2 Antibody [NBP2-93699] - Paraffin-embedded rat ovary using SLC7A2.
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Western Blot: CRAT Antibody [NBP1-86616] - Analysis using Anti-CRAT antibody NBP1-86616 (A) shows similar pattern to independent antibody NBP1-86615 (B).
Western Blot: CRAT Antibody [NBP1-86616] - Analysis in human cell line TD47D.
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Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.
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(1 Publication)
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Western Blot: Paxillin [p Tyr118] Antibody [NBP2-24459] - Analysis of phospho Paxillin (Y118) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 118 and 2) an unphosphorylated tyrosine at position 118, using paxillin phospho antibody at 0.025 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.
Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
(3 Reviews)
(56 Publications)
Species: Hu, Mu, Rt, Po
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
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Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120328), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
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Immunocytochemistry/Immunofluorescence: TRPV6 Antibody [NBP1-74138] - hRetinal pigment epithelial cells Green: primary Red: nuclear Primary Dilution: 1:200 Secondary Antibody: goat anti-rabbit-Alexa 488 Secondary Dilution: 1:500 Image Submitted by: Brian Kennedy Indiana University Northwest
Western Blot: TRPV6 Antibody [NBP1-74138] - Rat Brain Lysate 1ug/ml Gel Concentration 12%
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AKT [p Ser473]
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Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Immunohistochemistry-Paraffin: ISYNA1 Antibody [NBP2-47415] - Staining of human placenta.
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Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry: nNOS Antibody [NB100-858] - Immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse caudate-putamen. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Scale bar: 50 um; inset: 20 um. Data obtained by Drs. Eva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human PTP beta / zeta /PTPRZ Monoclonal Antibody (Catalog # MAB2688) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PTP beta / zeta /PTPRZ at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Publications for GIT1 Antibody (NBP2-22423)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: ICC/IF, IHC-F, IP, KO, WB.


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ICC/IF
(1)
IHC-F
(1)
IP
(1)
KO
(1)
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(3)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GIT1 Antibody (NBP2-22423) (0)

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Control Lysate(s)

GIT1 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-22423AF405
Alexa Fluor 488 NBP2-22423AF488
Alexa Fluor 647 NBP2-22423AF647
Alexa Fluor 700 NBP2-22423AF700
Biotin NBP2-22423B
DyLight 350 NBP2-22423UV
DyLight 405 NBP2-22423V
DyLight 488 NBP2-22423G
DyLight 550 NBP2-22423R
DyLight 650 NBP2-22423C
DyLight 680 NBP2-22423FR
DyLight 755 NBP2-22423IR
FITC NBP2-22423F
HRP NBP2-22423H
Janelia Fluor 549 NBP2-22423JF549
Janelia Fluor 646 NBP2-22423JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol GIT1
Uniprot