Calbindin D/S100G Antibody [Unconjugated]

Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Calbindin D was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Calbindin D/S100G Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Calbindin D
  Ala2-Asn261
  Accession # P05937
• Specificity: Detects human Calbindin D in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100G
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calbindin D/S100G Antibody [Unconjugated]

• CABP
• CABP;CABP1;CABP9K;CALB3;Protein S100-G
• CABP1
• CABP28K
• CABP9K
• Calbindin 3
• Calbindin D
• Calbindin-D
• Calbindin-D9K
• S100G

Background

Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calbindin D/S100G Antibody (AF3320)(8)

Publications using AF3320

• Viventi S, Frausin S, Howden SE et al. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia Stem cells translational medicine 3/18/2021 [PMID: 33734599]
• Ashley J. Swain, Adriana Galvan, Thomas Wichmann, Yoland Smith Structural plasticity of GABAergic and glutamatergic networks in the motor thalamus of parkinsonian monkeys Journal of Comparative Neurology 2020-06-01 [PMID: 31808567]
• M Zhang, Y Wang, J Geng, S Zhou, B Xiao Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice Cell Rep, 2019-02-05;26(6):1419-1431.e4. 2019-02-05 [PMID: 30726728] (IHC, Mouse)
• Allison M Bradbury Cerebrospinal fluid calbindin D concentration as a biomarker of cerebellar disease progression in Niemann-Pick type C1 disease J Pharmacol Exp Ther, 2016-06-15;0(0):. 2016-06-15 [PMID: 27307499] (ELISA Development (Detection), Human)
• Bifsha P, Yang J, Fisher R, Drouin J Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet, 2014-12-11;10(12):e1004863. 2014-12-11 [PMID: 25501001] (IHC-P, Mouse)
• Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2014-11-24;18(1):145-53. 2014-11-24 [PMID: 25420068] (IHC, Mouse)
• Di Giovannantonio , Luca G, Di Salvio , Michela, Omodei , Daniela, Prakash , Nilima, Wurst , Wolfgang, Pierani , Alessand, Acampora , Dario, Simeone , Antonio Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development, 2013-12-11;141(2):377-88. 2013-12-11 [PMID: 24335253] (IHC, Mouse)
• Di Salvio M, Di Giovannantonio LG, Acampora D Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat. Neurosci., 2010-11-07;13(12):1481-8. 2010-11-07 [PMID: 21057506] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: S100G
• Uniprot:
  • P05937()