Home » Calbindin D/S100G » Calbindin D/S100G Antibodies » Calbindin D/S100G Antibody [Unconjugated]

Calbindin D/S100G Antibody [Unconjugated]

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Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by ...read more
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3320-SP
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Calbindin D/S100G Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Calbindin D
Ala2-Asn261
Accession # P05937
Specificity
Detects human Calbindin D in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100G
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 5 ug/mL
Publications
Read Publications using
AF3320 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calbindin D/S100G Antibody [Unconjugated]

  • CABP
  • CABP1
  • CABP28K
  • CABP9K
  • Calbindin 3
  • Calbindin D
  • Calbindin-D
  • Calbindin-D9K
  • S100G

Background

Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. NBP2-50028 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.
Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.
Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with NBP2-50028 at 1:5,000. Lane 1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin.
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Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody [NBP2-14871] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 stained by Calmodulin 2 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: Calmodulin 2 Antibody [NBP2-14871] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calmodulin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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NB120-2860
Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
(2 Publications)
Species: Ba, Bv, Ch, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Calmodulin 3
Antibody
NBP2-15669
Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Upregulation of miRNA inhibits the expression level of target genes in HK-2 cells and CKD patients. Western Blot shows that hsa-miR-4709-3p inhibit the protein level of ITGB8 and CALM3 respectively. NC: scrambled negative control transfection;4709: hsa-miR-4709-3p transfection. Image collected and cropped by CiteAb from the following publication (https://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-019-1512-x) licensed under a CC-BY licence.
Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
(1 Publication)
Species: Hu, Mu, Rt
Applications: IHC, WB
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Western Blot: TRPV6 Antibody [NBP1-74138] - Rat Brain Lysate 1ug/ml Gel Concentration 12%
Immunocytochemistry/Immunofluorescence: TRPV6 Antibody [NBP1-74138] - hRetinal pigment epithelial cells Green: primary Red: nuclear Primary Dilution: 1:200 Secondary Antibody: goat anti-rabbit-Alexa 488 Secondary Dilution: 1:500 Image Submitted by: Brian Kennedy Indiana University Northwest
Western Blot: TRPV6 Antibody [NBP1-74138] - Sample Type: hRetinal pigment epithelial cells, 4 individual donors (20ug) Primary Dilution: 1:1000 Secondary Antibody: goat anti-rabbit-AP Secondary Dilution: 1:2000 Image Submitted by: Brian Kennedy Indiana University Northwest
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CYP27B1 GST (N-Term) ...
H00001594-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
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VDR/NR1I1/Vitamin
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Flow Cytometry: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of Hela cells with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
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Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells.
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Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
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Western blot shows lysates of human parathyroid tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTH at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Flow Cytometry: S100B Antibody (15F9NB) [NBP2-45267] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (Orange) stained at a dilution of 1:500. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary control (Blue).
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Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495] - Analysis in human urinary bladder and fallopian tube tissues using NBP1-85495 antibody. Corresponding CYP24A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495] - Staining of human colon shows no postivity in glandular cells as expected.
Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495] - Staining of human fallopian tube shows no postivity in glanduar cells as expected.
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Progesterone
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Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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PMCA1 Antibody
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Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.
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Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Saos‑2 human osteosarcoma cell line was stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Human osteoblasts were stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Osteocalcin was detected in human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(4 Reviews)
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NBP1-87103
Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Sodium Calcium
Exchanger 1/NCX1 Anti ...
NBP2-94316
Immunohistochemistry-Paraffin: Sodium Calcium Exchanger 1/NCX1 Antibody [NBP2-94316] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Sodium Calcium Exchanger 1/NCX1 Rabbit pAb (NBP2-94316) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Sodium Calcium Exchanger 1/NCX1 Antibody [NBP2-94316] - Immunohistochemistry of paraffin-embedded mouse spleen using Sodium Calcium Exchanger 1/NCX1 Rabbit pAb (NBP2-94316) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Sodium Calcium Exchanger 1/NCX1 Antibody [NBP2-94316] - Immunohistochemistry of paraffin-embedded rat stomach using Sodium Calcium Exchanger 1/NCX1 Rabbit pAb (NBP2-94316) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
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Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
(2 Reviews)
(3 Publications)
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB

Publications for Calbindin D/S100G Antibody (AF3320)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA Development (Detection), IHC, IHC-P.


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ELISA Development (Detection)
(1)
IHC
(3)
IHC-P
(2)
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Human
(1)
Mouse
(5)
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Showing Publications 1 - 6 of 6.
Publications using AF3320 Applications Species
M Zhang, Y Wang, J Geng, S Zhou, B Xiao Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice Cell Rep, 2019;26(6):1419-1431.e4. 2019 [PMID: 30726728] (IHC, Mouse) IHC Mouse
Allison M Bradbury Cerebrospinal fluid calbindin D concentration as a biomarker of cerebellar disease progression in Niemann-Pick type C1 disease J Pharmacol Exp Ther, 2016;0(0):. 2016 [PMID: 27307499] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Bifsha P, Yang J, Fisher R, Drouin J Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet, 2014;10(12):e1004863. 2014 [PMID: 25501001] (IHC-P, Mouse) IHC-P Mouse
Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2015;18(1):145-53. 2015 [PMID: 25420068] (IHC, Mouse) IHC Mouse
Di Giovannantonio , Luca G, Di Salvio , Michela, Omodei , Daniela, Prakash , Nilima, Wurst , Wolfgang, Pierani , Alessand, Acampora , Dario, Simeone , Antonio Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development, 2014;141(2):377-88. 2014 [PMID: 24335253] (IHC, Mouse) IHC Mouse
Di Salvio M, Di Giovannantonio LG, Acampora D Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat. Neurosci., 2010;13(12):1481-8. 2010 [PMID: 21057506] (IHC-P, Mouse) IHC-P Mouse

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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.
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Bioinformatics

Gene Symbol S100G
Uniprot