tropomyosin-3 Antibody - BSA Free

Wild-type (WT) and Tropomyosin 3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Tropomyosin 3 antibody (NBP2-20732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Ca, RM
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

tropomyosin-3 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit tropomyosin-3 Antibody - BSA Free (NBP2-20732) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human tropomyosin-3 Recombinant protein.
• Localization: Cytoplasm; cytoskeleton
• Predicted Species: Mouse (99%), Rat (99%), Porcine (100%), Rhesus Macaque (93%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPM3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), , Chicken (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for tropomyosin-3 Antibody - BSA Free

• cytoskeletal tropomyosin TM30
• FLJ41118
• gamma-tropomyosin
• heat-stable cytoskeletal protein 30 kDa
• hscp30
• hTM5
• MGC14582
• MGC3261
• MGC72094
• NEM1
• OK/SW-cl.5
• TM3
• TM30
• TM30nm
• TM-5
• TPMsk3
• TRK
• tropomyosin 3
• tropomyosin alpha-3 chain
• tropomyosin gamma
• tropomyosin-3
• tropomyosin-5

Background

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TPM3