TROP-2 Antibody (77220) [Unconjugated]

PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human TROP‑2 Monoclonal Antibody (Catalog # MAB650, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

Flow cytometry histograms of A431 wild-type cells (left graph) and A431-TROP2 Knock-out cells (right graph). Both cell lines were incubated with FAB650P before the analysis. Image from a verified customer review. The customer used the PE-conjugated version of MAB650, which is listed under FAB650P.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 77220
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TROP-2 Antibody (77220) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TROP-2
  His27-Thr274
  Accession # P09758
• Specificity: Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TACSTD2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody (77220) [Unconjugated]

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (MAB650)(7)

Publications using MAB650

• TXA Pham, A Panda, H Kagawa, SK To, C Ertekin, G Georgolopo, SSFA van Knippe, RN Allsop, A Bruneau, JS Chui, L Vanheer, A Janiszewsk, J Chappell, M Oberhuemer, RS Tchinda, I Talon, S Khodeer, J Rossant, F Lluis, L David, N Rivron, BP Balaton, V Pasque Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells Cell Stem Cell, 2022-09-01;29(9):1346-1365.e10. 2022-09-01 [PMID: 36055191] (ICC, Human)
• DW Zijlmans, I Talon, S Verhelst, A Bendall, K Van Nerum, A Javali, AA Malcolm, SSFA van Knippe, L Biggins, SK To, A Janiszewsk, D Admiraal, R Knops, N Corthout, BP Balaton, G Georgolopo, A Panda, NV Bhanu, AJ Collier, C Fabian, RN Allsop, J Chappell, TXA Pham, M Oberhuemer, C Ertekin, L Vanheer, P Athanasoul, F Lluis, D Deforce, JH Jansen, BA Garcia, M Vermeulen, N Rivron, M Dhaenens, H Marks, PJ Rugg-Gunn, V Pasque Integrated multi-omics reveal polycomb repressive complex 2 restricts human trophoblast induction Nature Cell Biology, 2022-06-13;24(6):858-871. 2022-06-13 [PMID: 35697783] (Flow Cytometry, Human)
• Bouchard G, Garcia-Marques FJ, Karacosta LG et al. Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge Cancer Research 2022-02-15 [PMID: 34853070]
• Y Hao, D Zhang, Y Guo, Z Fu, D Yu, G Guan miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development J Cancer, 2020-03-05;11(11):3375-3386. 2020-03-05 [PMID: 32231744] (Western Blot, Human)
• Yamaguchi M, Nishii Y, Nakamura K, Aoki H, Hirai S, Uchida H, Sakuma Y, Hamada H Development of a sensitive screening method for selecting monoclonal antibodies to be internalized by cells. Biochem Biophys Res Commun, 2014-11-01;454(4):600-603. 2014-11-01 [PMID: 25450699] (Bioassay, Human)
• Nakatsukasa M, Kawasaki S, Yamasaki K Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy. Am. J. Pathol., 2010-07-22;177(3):1344-55. 2010-07-22 [PMID: 20651236] (Immunoprecipitation, Human)
• Frederick BA, Helfrich BA, Coldren CD, Zheng D, Chan D, Bunn PA, Raben D Epithelial to mesenchymal transition predicts gefitinib resistance in cell lines of head and neck squamous cell carcinoma and non-small cell lung carcinoma. Mol. Cancer Ther., 2007-05-31;6(6):1683-91. 2007-05-31 [PMID: 17541031] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • P09758()