TROP-2 Antibody (77220) [Phycoerythrin]

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 PE-conjugated Monoclonal Antibody (Catalog # FAB650P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow cytometry histograms of A431 wild-type cells (left graph) and A431-TROP2 Knock-out cells (right graph). Both cell lines were incubated with FAB650P before the analysis. Only wild-type cells show a signal. Image from a verified customer review.

• Reactivity: Hu
• Applications: Flow
• Clone: 77220
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

TROP-2 Antibody (77220) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TROP-2
  His27-Thr274
  Accession # P09758
• Specificity: Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TACSTD2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody (77220) [Phycoerythrin]

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

Human TROP-2, also known as Tumor Associated Calcium Signal Transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (FAB650P)(2)

Publications using FAB650P

• Guo, P;Huang, J;Zhu, B;Huang, AC;Jiang, L;Fang, J;Moses, MA; A rationally designed ICAM1 antibody drug conjugate eradicates late-stage and refractory triple-negative breast tumors in vivo Science advances 2023-05-05 [PMID: 37146146] (Flow Cytometry, ICC, Human)
• Andrew S. Goldstein, Justin M. Drake, Daina L. Burnes, David S. Finley, Hong Zhang, Robert E. Reiter et al. Purification and direct transformation of epithelial progenitor cells from primary human prostate Nature Protocols 2011-05-01 [PMID: 21527922]

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • P09758()