TrkB Antibody [Unconjugated]

Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TrkB at approximately 90-100 & 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TrkB at approximately 95 kDa and 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkB at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF397) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Baf3 cells transfected with Human TrkB (filled histogram) vs Irrelevant transfectant (open histogram) were stained with Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF397) followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TrkB Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TrkB
• Specificity: Detects human TrkB in direct ELISA. Detects human, mouse, and rat TrkB Western blots. In direct ELISA and Western blots, less than 1% cross-reactivity with recombinant human (rh) TrkC, rhNT‑3, rhNT‑4, rhBDNF, and rh beta ‑NGF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NTRK2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 0.25-0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkB Antibody [Unconjugated]

• BDNF/NT-3 growth factors receptor
• EC 2.7.10
• EC 2.7.10.1
• GP145-TrkB
• Neurotrophic tyrosine kinase receptor type 2
• neurotrophic tyrosine kinase, receptor, type 2
• NTRK2
• TrkB tyrosine kinase
• TrkB
• trk-B
• TRKBBDNF-tropomyosine receptor kinase B
• tyrosine kinase receptor B

Background

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT-4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
3. Soppet, D. et al. (1991) Cell 65:895.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkB Antibody (AF397)(3)

Publications using AF397

• ME Johnson, XF Zhou, L Bobrovskay The effects of rotenone on TH, BDNF and BDNF-related proteins in the brain and periphery: relevance to early Parkinson's disease J. Chem. Neuroanat., 2019-01-25;0(0):. 2019-01-25 [PMID: 30690135] (Western Blot, Rat)
• Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013-03-20;33(12):5399-410. 2013-03-20 [PMID: 23516305] (ICC, Western Blot, Human)
• Dewitt J, Ochoa V, Urschitz J, Elston M, Moisyadi S, Nishi R Constitutively active TrkB confers an aggressive transformed phenotype to a neural crest-derived cell line. Oncogene, 2013-03-04;33(8):977-85. 2013-03-04 [PMID: 23455321] (Western Blot, Human)

Bioinformatics

• Gene Symbol: NTRK2