TrkB [p Tyr817] Antibody (SC0556)

Western Blot: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Western blot analysis of TrkB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Immunocytochemistry/Immunofluorescence: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Staining phospho -TrkB(Y817) in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded mouse pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Staining phospho -TrkB(Y817) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded human kidney tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded human pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded mouse heart tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: SC0556
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TrkB [p Tyr817] Antibody (SC0556) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr817 of Human TrkB aa 789-822 / 822. (SwissProt: Q16620 Human; SwissProt: P15209 Mouse; SwissProt: Q63604 Rat)
• Modification: p Tyr817
• Localization: Cell membrane, Endosome membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NTRK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TrkB [p Tyr817] Antibody (SC0556)

• BDNF/NT-3 growth factors receptor
• EC 2.7.10
• EC 2.7.10.1
• GP145-TrkB
• Neurotrophic tyrosine kinase receptor type 2
• neurotrophic tyrosine kinase, receptor, type 2
• NTRK2
• TrkB tyrosine kinase
• TrkB
• trk-B
• TRKBBDNF-tropomyosine receptor kinase B
• tyrosine kinase receptor B

Background

TrkB is a tyrosine-protein kinase receptor involved in the development and/or maintenance of the nervous system. TrkB is a receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). The major substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkB Antibody (NBP2-67578)(1)

Publication using NBP2-67578

• Park GB, Choi S, Yoon YS, Kim D TrkB/C-induced HOXC6 activation enhances the ADAM8-mediated metastasis of chemoresistant colon cancer cells Molecular medicine reports 2021-06-01 [PMID: 33846772]

Bioinformatics

• Gene Symbol: NTRK2