TrkA Antibody [DyLight 680]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: DyLight 680

TrkA Antibody [DyLight 680] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat TrkA
  Ala33-Pro418
  Accession # P35739
• Specificity: Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human TrkA and less than 1% cross-reactivity with recombinant mouse (rm) TrkB and rmTrkC is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NTRK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product AF1056. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for TrkA Antibody [DyLight 680]

• DKFZp781I14186
• EC 2.7.10
• EC 2.7.10.1
• MTChigh affinity nerve growth factor receptor
• Neurotrophic tyrosine kinase receptor type 1
• neurotrophic tyrosine kinase, receptor, type 1
• NTRK1
• NTRK-1
• p140-TrkA
• TRK1-transforming tyrosine kinase protein
• TrkA
• Trk-A
• TRKAOncogene TRK
• TRKTRK1
• tyrosine kinase receptor A

Background

The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TrkA Antibody (AF1056Y). (Showing 1 - 1 of 1 FAQ).

1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
   • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

Bioinformatics

• Gene Symbol: NTRK1