TRIM5 alpha Antibody

Images

 
Western Blot: TRIM5 alpha Antibody [NBP1-77207] - Western blot analysis of TRIM5 alpha expression in (A) human stomach (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: TRIM5 alpha Antibody [NBP1-77207] - Human uterus tissue with TRIM5 alpha antibody at 2 ug/ml.
Western Blot: TRIM5 alpha Antibody [NBP1-77207] - Human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TRIM5 alpha Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha. The immunogen is located within amino acids 390 - 440 of TRIM5 alpha.
Specificity
TRIM5 alpha antibody will not cross-react with other isoforms of TRIM5.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Application Notes
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 1 to 2 mg/ml. A band at approximately 55 kDa can be detected.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM5 alpha Antibody

  • EC 6.3.2
  • EC 6.3.2.-
  • RING finger protein 88
  • RNF88tripartite motif protein TRIM
  • TRIM5alpha
  • tripartite motif containing 5
  • tripartite motif protein TRIM5
  • tripartite motif-containing 5
  • tripartite motif-containing protein 5

Background

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5alpha variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5alpha acts as a natural barrier to cross-species retrovirus transmission.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM5
Uniprot