TRIM5 alpha Antibody - BSA Free

Western Blot: TRIM5 alpha Antibody [NBP1-77207] - Western blot analysis of TRIM5 alpha expression in (A) human stomach (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 ug/ml.

Western Blot: TRIM5 alpha Antibody [NBP1-77207] - Human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml.

Immunohistochemistry: TRIM5 alpha Antibody - BSA Free [NBP1-77207] - Immunohistochemistry of TRIM5 alpha in human uterus tissue with TRIM5 alpha antibody at 2 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRIM5 alpha Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha. The immunogen is located within amino acids 390 - 440 of TRIM5 alpha. Amino Acid Squence: GLQEGVKYSVFQDG
• Specificity: TRIM5 alpha antibody will not cross-react with other isoforms of TRIM5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 2 ug/ml
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TRIM5 alpha Antibody - BSA Free

• EC 6.3.2
• EC 6.3.2.-
• RING finger protein 88
• RNF88tripartite motif protein TRIM
• TRIM5alpha
• tripartite motif containing 5
• tripartite motif protein TRIM5
• tripartite motif-containing 5
• tripartite motif-containing protein 5

Background

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5alpha variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5alpha acts as a natural barrier to cross-species retrovirus transmission.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM5
• Uniprot:
  • NP_149023(Human)