TRIM5 alpha Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 647

TRIM5 alpha Antibody [Alexa Fluor™ Plus 647] Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha. The immunogen is located within amino acids 390 - 440 of TRIM5 alpha. Amino Acid Squence: GLQEGVKYSVFQDG
• Specificity: TRIM5 alpha antibody will not cross-react with other isoforms of TRIM5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TRIM5 alpha Antibody [Alexa Fluor™ Plus 647]

• EC 6.3.2
• EC 6.3.2.-
• RING finger protein 88
• RNF88tripartite motif protein TRIM
• TRIM5alpha
• tripartite motif containing 5
• tripartite motif protein TRIM5
• tripartite motif-containing 5
• tripartite motif-containing protein 5

Background

TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5 alpha, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM5