TRIM25 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin

TRIM25 Antibody [Biotin] Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TRIM25. The immunogen is located within amino acids 40 - 90 of TRIM25. Amino Acid Squence: CRAVYQARPQLHKNT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM25
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Alternate Names for TRIM25 Antibody [Biotin]

• EC 6.3.2.19
• EC 6.3.2.n3
• EFP
• EFPZ147
• Estrogen-responsive finger protein
• RING finger protein 147
• RNF147
• RNF147E3 ubiquitin/ISG15 ligase TRIM25
• TRIM25
• tripartite motif containing 25
• tripartite motif-containing 25
• Tripartite motif-containing protein 25
• Ubiquitin/ISG15-conjugating enzyme TRIM25
• zinc finger protein 147 (estrogen-responsive finger protein)
• Zinc finger protein 147
• zinc finger protein-147
• ZNF147
• ZNF147tripartite motif protein TRIM25

Background

TRIM25 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM25