TRIM25 Antibodies
We offer TRIM25 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each TRIM25 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TRIM25 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the TRIM25 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TRIM25 polyclonal antibodies and browse our TRIM25 monoclonal antibody catalog.
Alternate Names for TRIM25 Antibodies
anti-TRIM25 antibody, anti-EC 6.3.2.19 antibody, anti-EC 6.3.2.n3 antibody, anti-EFPZ147 antibody, anti-Estrogen-responsive finger protein antibody, anti-RING finger protein 147 antibody, anti-RNF147E3 ubiquitin/ISG15 ligase TRIM25 antibody, anti-tripartite motif containing 25 antibody, anti-tripartite motif-containing 25 antibody, anti-Tripartite motif-containing protein 25 antibody, anti-Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody, anti-zinc finger protein 147 (estrogen-responsive finger protein) antibody, anti-Zinc finger protein 147 antibody, anti-zinc finger protein-147 antibody, anti-ZNF147tripartite motif protein TRIM25 antibody
14 Results for "trim25" in Primary Antibodies
![Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP2-20710-img0006.jpg "Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP2-20710] - Human colon carcinoma, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP2-20710-img0002.jpg)
![Western Blot: TRIM25 Antibody [NBP2-20710] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP2-20710-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis of UPR signaling, including phospho-JNK/JNK and phospho-eIF2alpha/eIF2alpha in HCT116 cells after stable knockdown of TRIM25 in HCT116 cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Knockdown-Validated-NBP2-20710-img0007.jpg)
![Immunohistochemistry: TRIM25 Antibody [NBP2-20710] - Knockdown of TRIM25 in HCC suppresses tumor growth in nude mice.a–c Huh7 cells stably expressing the indicated shRNAs (shNC, shTRIM25-1 & shTRIM25-2) were subcutaneously injected in nude mice respectively. Shown are average tumor volumes over time (n = 8) (a) & representative image (b) & weights (c) of tumors at day 25. d Kaplan–Meier analysis of overall survival percentage in nude mice inoculated with Huh7-shNC or Huh7-shTRIM25. The statistical significance was assessed using two-sided log-rank test according to mice with depletion of control or TRIM25. e, f Representative images (e) of IHC staining & the relative IHC scores (n = 8) (f) of TRIM25, Ki67, Keap1 & Nrf2 in HCC tissues of mice inoculated with Huh7-shNC or Huh7-shTRIM25-1&-2. Scale bar, 50 μm. g, h Representative IHC staining images (g) & statistical data (h) of TRIM25, Keap1 & Nrf2 expression in HCC tissues (TRIM25 low expression group, n = 45 & TRIM25 high expression group, n = 45). Scale bar, 50 μm. For a, c, f, & h, data represent the mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31953436), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20710_rabbit-polyclonal-trim25-antibody-310202416121958.jpg)
![Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Western-Blot-NBP2-61814-img0007.jpg "Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Immunocytochemistry-Immunofluorescence-NBP2-61814-img0003.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Immunohistochemistry-NBP2-61814-img0005.jpg)
![Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Western-Blot-NBP2-61814-img0006.jpg)
![Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Western-Blot-NBP2-61814-img0008.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Immunohistochemistry-NBP2-61814-img0004.jpg)
![ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-ELISA-NBP2-61814-img0001.jpg)
![ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-ELISA-NBP2-61814-img0002.jpg)
![Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Western-Blot-NBP2-61820-img0007.jpg "Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Immunocytochemistry-Immunofluorescence-NBP2-61820-img0003.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of paraffin-embedded esophageal cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Immunohistochemistry-NBP2-61820-img0005.jpg)
![Flow Cytometry: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-ELISA-NBP2-61820-img0002.jpg)
![Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Western-Blot-NBP2-61820-img0006.jpg)
![Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mouse mAb against MCF-7 (1), Hela (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Western-Blot-NBP2-61820-img0008.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of paraffin-embedded cervical cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Immunohistochemistry-NBP2-61820-img0004.jpg)
![ELISA: TRIM25 Antibody (5B5B10) [NBP2-61820] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-ELISA-NBP2-61820-img0001.jpg)
![Western Blot: TRIM25 Antibody [NBP1-84891] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-84891-img0003.jpg "Western Blot: TRIM25 Antibody [NBP1-84891] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-84891] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84891-img0005.jpg)
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP1-84891-img0001.jpg)
![Western Blot: TRIM25 Antibody [NBP1-32050] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-32050-img0009.jpg "Western Blot: TRIM25 Antibody [NBP1-32050] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-32050] - HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: TRIM25 protein stained by TRIM25 antibody [N2C1], Internal diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32050-img0006.jpg)
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-32050] - Human breast cancer, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP1-32050-img0002.jpg)
![Western Blot: TRIM25 Antibody (SR2171) [NBP3-21633] - Western blot analysis using NBP3-21633 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21633_rabbit-trim25-mab-sr2171-6720239533749.jpg "Western Blot: TRIM25 Antibody (SR2171) [NBP3-21633] - Western blot analysis using NBP3-21633 in HeLa cell lysate.")
![Western Blot: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of TRIM25 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/TRIM25-Antibody-5C3-Western-Blot-H00007706-M03-img0006.jpg "Western Blot: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of TRIM25 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of monoclonal antibody to TRIM25 on HeLa cell. Antibody concentration 20 ug/ml](http://images.novusbio.com/fullsize/TRIM25-Antibody-5C3-Immunocytochemistry-Immunofluorescence-H00007706-M03-img0004.jpg)
![Immunohistochemistry-Paraffin: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of monoclonal antibody to TRIM25 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/TRIM25-Antibody-5C3-Immunohistochemistry-Paraffin-H00007706-M03-img0005.jpg)
![Western Blot: TRIM25 Antibody [NBP1-76825] - HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-76825-img0001.jpg "Western Blot: TRIM25 Antibody [NBP1-76825] - HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76825] - HeLa cells with TRIM25 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76825-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76825] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76825_rabbit-polyclonal-trim25-antibody-271120241937797.jpg)
![Western Blot: TRIM25 Antibody [NBP1-76826] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-76826-img0004.jpg "Western Blot: TRIM25 Antibody [NBP1-76826] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76826] - HeLa cells with TRIM25 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76826-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76826] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76826_rabbit-polyclonal-trim25-antibody-2711202419313262.jpg)
![Western Blot: TRIM25 Antibody (5F12) [H00007706-M02] - TRIM25 monoclonal antibody (M02), clone 5F12 Analysis of TRIM25 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5F12-Western-Blot-H00007706-M02-img0005.jpg "Western Blot: TRIM25 Antibody (5F12) [H00007706-M02] - TRIM25 monoclonal antibody (M02), clone 5F12 Analysis of TRIM25 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5F12) [H00007706-M02] - Analysis of monoclonal antibody to TRIM25 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5F12-Immunocytochemistry-Immunofluorescence-H00007706-M02-img0004.jpg)
![ELISA: TRIM25 Antibody (5F12) [H00007706-M02] - Detection limit for recombinant GST tagged TRIM25 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5F12-ELISA-H00007706-M02-img0006.jpg)
![Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TRIM25-Antibody-4Q0D2-Western-Blot-NBP3-16382-img0001.jpg "Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16382_rabbit-trim25-mab-4q0d2-52202511383780.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody (4Q0D2) [TRIM25] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16382_rabbit-trim25-mab-4q0d2-52202517172871.jpg)
![Western Blot: TRIM25 Antibody (JE57-43) [NBP3-33053] - Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: 293T cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33053) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33053_rabbit-trim25-mab-je57-43-288202412493473.jpg "Western Blot: TRIM25 Antibody (JE57-43) [NBP3-33053] - Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: 293T cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33053) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (JE57-43) [NBP3-33053] - Immunocytochemistry analysis of HeLa cells labeling TRIM25 with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33053_rabbit-trim25-mab-je57-43-29202410484889.jpg)