TRIM25 Antibody - BSA Free

Western Blot: TRIM25 Antibody [NBP1-76826] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76826] - HeLa cells with TRIM25 antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76826] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRIM25 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRIM25 Antibody - BSA Free (NBP1-76826) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TRIM25. The immunogen is located within amino acids 40 - 90 of TRIM25. Amino Acid Squence: CRAVYQARPQLHKNT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM25
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  TRIM25 Peptide (NBP1-76826PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TRIM25 Antibody - BSA Free

• EC 6.3.2.19
• EC 6.3.2.n3
• EFP
• EFPZ147
• Estrogen-responsive finger protein
• RING finger protein 147
• RNF147
• RNF147E3 ubiquitin/ISG15 ligase TRIM25
• TRIM25
• tripartite motif containing 25
• tripartite motif-containing 25
• Tripartite motif-containing protein 25
• Ubiquitin/ISG15-conjugating enzyme TRIM25
• zinc finger protein 147 (estrogen-responsive finger protein)
• Zinc finger protein 147
• zinc finger protein-147
• ZNF147
• ZNF147tripartite motif protein TRIM25

Background

TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM25
• Uniprot:
  • NP_005073(Human)