| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61814-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61820-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84891-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21633-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76825-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76826-0.025mg | |||
![Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP2-20710-img0006.jpg "Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP2-20710] - Human colon carcinoma, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP2-20710-img0002.jpg)
![Western Blot: TRIM25 Antibody [NBP2-20710] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP2-20710-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis of UPR signaling, including phospho-JNK/JNK and phospho-eIF2alpha/eIF2alpha in HCT116 cells after stable knockdown of TRIM25 in HCT116 cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Knockdown-Validated-NBP2-20710-img0007.jpg)
![Immunohistochemistry: TRIM25 Antibody [NBP2-20710] - Knockdown of TRIM25 in HCC suppresses tumor growth in nude mice.a–c Huh7 cells stably expressing the indicated shRNAs (shNC, shTRIM25-1 & shTRIM25-2) were subcutaneously injected in nude mice respectively. Shown are average tumor volumes over time (n = 8) (a) & representative image (b) & weights (c) of tumors at day 25. d Kaplan–Meier analysis of overall survival percentage in nude mice inoculated with Huh7-shNC or Huh7-shTRIM25. The statistical significance was assessed using two-sided log-rank test according to mice with depletion of control or TRIM25. e, f Representative images (e) of IHC staining & the relative IHC scores (n = 8) (f) of TRIM25, Ki67, Keap1 & Nrf2 in HCC tissues of mice inoculated with Huh7-shNC or Huh7-shTRIM25-1&-2. Scale bar, 50 μm. g, h Representative IHC staining images (g) & statistical data (h) of TRIM25, Keap1 & Nrf2 expression in HCC tissues (TRIM25 low expression group, n = 45 & TRIM25 high expression group, n = 45). Scale bar, 50 μm. For a, c, f, & h, data represent the mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31953436), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20710_rabbit-polyclonal-trim25-antibody-310202416121958.jpg)
![Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Western-Blot-NBP2-61814-img0007.jpg "Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Immunocytochemistry-Immunofluorescence-NBP2-61814-img0003.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Immunohistochemistry-NBP2-61814-img0005.jpg)
![Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Western-Blot-NBP2-61814-img0006.jpg)
![Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Western-Blot-NBP2-61814-img0008.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-Immunohistochemistry-NBP2-61814-img0004.jpg)
![ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-ELISA-NBP2-61814-img0001.jpg)
![ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B12-ELISA-NBP2-61814-img0002.jpg)
![Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Western-Blot-NBP2-61820-img0007.jpg "Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Immunocytochemistry-Immunofluorescence-NBP2-61820-img0003.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of paraffin-embedded esophageal cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Immunohistochemistry-NBP2-61820-img0005.jpg)
![Flow Cytometry: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-ELISA-NBP2-61820-img0002.jpg)
![Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Western-Blot-NBP2-61820-img0006.jpg)
![Western Blot: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis using TRIM25 mouse mAb against MCF-7 (1), Hela (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Western-Blot-NBP2-61820-img0008.jpg)
![Immunohistochemistry: TRIM25 Antibody (5B5B10) [NBP2-61820] - Analysis of paraffin-embedded cervical cancer tissues using TRIM25 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-Immunohistochemistry-NBP2-61820-img0004.jpg)
![ELISA: TRIM25 Antibody (5B5B10) [NBP2-61820] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/TRIM25-Antibody-5B5B10-ELISA-NBP2-61820-img0001.jpg)
![Western Blot: TRIM25 Antibody [NBP1-84891] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-84891-img0003.jpg "Western Blot: TRIM25 Antibody [NBP1-84891] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-84891] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84891-img0005.jpg)
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP1-84891-img0001.jpg)
![Western Blot: TRIM25 Antibody [NBP1-32050] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-32050-img0009.jpg "Western Blot: TRIM25 Antibody [NBP1-32050] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-32050] - HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: TRIM25 protein stained by TRIM25 antibody [N2C1], Internal diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32050-img0006.jpg)
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-32050] - Human breast cancer, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP1-32050-img0002.jpg)
![Western Blot: TRIM25 Antibody (SR2171) [NBP3-21633] - Western blot analysis using NBP3-21633 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21633_rabbit-trim25-mab-sr2171-6720239533749.jpg "Western Blot: TRIM25 Antibody (SR2171) [NBP3-21633] - Western blot analysis using NBP3-21633 in HeLa cell lysate.")
![Western Blot: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of TRIM25 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/TRIM25-Antibody-5C3-Western-Blot-H00007706-M03-img0006.jpg "Western Blot: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of TRIM25 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of monoclonal antibody to TRIM25 on HeLa cell. Antibody concentration 20 ug/ml](http://images.novusbio.com/fullsize/TRIM25-Antibody-5C3-Immunocytochemistry-Immunofluorescence-H00007706-M03-img0004.jpg)
![Immunohistochemistry-Paraffin: TRIM25 Antibody (5C3) [H00007706-M03] - Analysis of monoclonal antibody to TRIM25 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/TRIM25-Antibody-5C3-Immunohistochemistry-Paraffin-H00007706-M03-img0005.jpg)
![Western Blot: TRIM25 Antibody [NBP1-76825] - HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-76825-img0001.jpg "Western Blot: TRIM25 Antibody [NBP1-76825] - HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76825] - HeLa cells with TRIM25 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76825-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76825] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76825_rabbit-polyclonal-trim25-antibody-271120241937797.jpg)
![Western Blot: TRIM25 Antibody [NBP1-76826] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-76826-img0004.jpg "Western Blot: TRIM25 Antibody [NBP1-76826] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76826] - HeLa cells with TRIM25 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76826-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76826] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76826_rabbit-polyclonal-trim25-antibody-2711202419313262.jpg)
![Western Blot: TRIM25 Antibody (5F12) [H00007706-M02] - TRIM25 monoclonal antibody (M02), clone 5F12 Analysis of TRIM25 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5F12-Western-Blot-H00007706-M02-img0005.jpg "Western Blot: TRIM25 Antibody (5F12) [H00007706-M02] - TRIM25 monoclonal antibody (M02), clone 5F12 Analysis of TRIM25 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5F12) [H00007706-M02] - Analysis of monoclonal antibody to TRIM25 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5F12-Immunocytochemistry-Immunofluorescence-H00007706-M02-img0004.jpg)
![ELISA: TRIM25 Antibody (5F12) [H00007706-M02] - Detection limit for recombinant GST tagged TRIM25 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRIM25-Antibody-5F12-ELISA-H00007706-M02-img0006.jpg)
![Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TRIM25-Antibody-4Q0D2-Western-Blot-NBP3-16382-img0001.jpg "Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16382_rabbit-trim25-mab-4q0d2-52202511383780.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody (4Q0D2) [TRIM25] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16382_rabbit-trim25-mab-4q0d2-52202517172871.jpg)
![Western Blot: TRIM25 Antibody (JE57-43) [NBP3-33053] - Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: 293T cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33053) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33053_rabbit-trim25-mab-je57-43-288202412493473.jpg "Western Blot: TRIM25 Antibody (JE57-43) [NBP3-33053] - Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: 293T cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33053) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (JE57-43) [NBP3-33053] - Immunocytochemistry analysis of HeLa cells labeling TRIM25 with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33053_rabbit-trim25-mab-je57-43-29202410484889.jpg)