14-3-3 sigma/Stratifin Antibody

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) HeLa cell lysate probed with 14-3-3 sigma antibody at 3 ug/ml.

• Reactivity: Hu, Am, Bv, Ca, Eq, Ma-Op, Pm, Mu, Rt, Po, Pm, Pm, Pm-Or, Rb, RM, Sh
• Applications: WB, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

14-3-3 sigma/Stratifin Antibody Summary

• Immunogen: A portion of amino acids 120-170 of human 14-3-3 sigma was used as the immunogen for this antibody.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Chimpanzee (100%), Orangutan (100%), Rabbit (100%), Rhesus Macaque (100%), Sheep (100%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10ug/ml
  • Knockdown Validated
  • Western Blot 3-5ug/ml~

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in White-cheeked Gibbon, and 89% in Opossum.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for 14-3-3 sigma/Stratifin Antibody

• 14-3-3 protein sigma
• 1433 sigma
• 14-3-3 sigma
• Epithelial cell marker protein 1
• HME1
• SFN
• stratifin
• YWHAS

Background

14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms (sigma epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigma is the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to irradiation and other DNA-damaging agents. 14-3-3 sigma is a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFN
• Uniprot:
  • P31947()