TRF-1 Antibody

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Western Blot: TRF-1 Antibody [NB100-891] - Analysis of TRF1 in HeLa lysate (RIPA buffer, 30ug total protein per lane) using this antibody at 2 ug/ml. Primary incubated for 12 hour. Detected using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TRF-1 Antibody Summary

Immunogen
Peptide with sequence C-TMKKLKLISSDSED corresponding to C-Terminus according to NP_003209.2, NP_059523.2.
Marker
Telomere Marker
Specificity
This antibody is expected to recognise isoform 1 (NP_059523.2) and isoform 2 (NP_003209.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TERF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 60-65 kDa band and 45 kDa band bands observed in HeLa lysate.
Positive Control
HeLa Lysate (NB800-PC1)
TRF-1 Lysate (NBL1-16814)
Publications
Read Publication using NB100-891.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRF-1 Antibody

  • NIMA-interacting protein 2
  • PIN-2
  • PIN2hTRF1-AS
  • Telomeric protein Pin2/TRF1
  • telomeric repeat binding factor (NIMA-interacting) 1
  • telomeric repeat binding factor 1
  • telomeric repeat binding protein 1
  • telomeric repeat-binding factor 1
  • TERF1
  • TRBF1
  • TRF1
  • TRF-1
  • TRF1FLJ41416
  • TRFt-TRF1
  • TTAGGG repeat-binding factor 1

Background

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRF-1 Antibody (NB100-891)(1)


Showing Publication 1 - 1 of 1.
Publication using NB100-891 Applications Species
Chong L, van Steensel B, Broccoli D et al. A human telomeric protein. Science 1995 Dec 8 [PMID: 7502076]

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TERF1
Entrez
Uniprot