TREM2 Antibody (237916) [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clone: 237916
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TREM2 Antibody (237916) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TREM2
  Leu19-Pro168
  Accession # Q99NH8
• Specificity: Detects mouse TREM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) TREM1, recombinant human TREM2, or rmTREM3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TREM2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM2 Antibody (237916) [Unconjugated]

• PLOSL2
• TREM2
• TREM-2
• Trem2a
• Trem2b
• Trem2c
• TREM-2triggering receptor expressed on myeloid cells 2a
• Triggering receptor expressed on monocytes 2
• triggering receptor expressed on myeloid cells 2

Background

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily . Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREM2 Antibody (MAB1729)(10)

Publications using MAB1729

• SF You, L Brase, F Filipello, AK Iyer, J Del-Aguila, J He, R D'Oliveira, J Budde, J Norton, J Gentsch, NM Dräger, SM Sattler, M Kampmann, L Piccio, JC Morris, RJ Perrin, E McDade, Dominantly, SM Paul, AG Cashikar, BA Benitez, O Harari, CM Karch MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state medRxiv : the preprint server for health sciences, 2023-02-08;0(0):. 2023-02-08 [PMID: 36798226] (ELISA Capture, Mouse)
• Wenhui Qiao, Yixing Chen, Jun Zhong, Benjamin J. Madden, Cristine M. Charlesworth, Yuka A. Martens et al. Trem2 H157Y increases soluble TREM2 production and reduces amyloid pathology Molecular Neurodegeneration 1/31/2023 [PMID: 36721205]
• Felix Oestereich, Noosha Yousefpour, Ethan Yang, Jasmine Phénix, Zari Saadati Nezhad, Albert Nitu et al. The cholesteryl ester transfer protein (CETP) raises cholesterol levels in the brain Journal of Lipid Research 9/1/2022 [PMID: 35921880]
• Kotredes KP, Oblak A, Pandey RS Et al. Uncovering Disease Mechanisms in a Novel Mouse Model Expressing Humanized APOE epsilon 4 and Trem2*R47H Frontiers in aging neuroscience 10/11/2021 [PMID: 34707490]
• VS Jadhav, PBC Lin, T Pennington, GV Di Prisco, AJ Jannu, G Xu, M Moutinho, J Zhang, BK Atwood, SS Puntambeka, SJ Bissel, AL Oblak, GE Landreth, BT Lamb Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice Mol Neurodegener, 2020-10-28;15(1):62. 2020-10-28 [PMID: 33115519] (ELISA Capture, Mouse)
• P Thornton, J Sevalle, MJ Deery, G Fraser, Y Zhou, S Ståhl, EH Franssen, RB Dodd, S Qamar, B Gomez Pere, LS Nicol, S Eketjäll, J Revell, C Jones, A Billinton, PH St George-, I Chessell, DC Crowther TREM2 shedding by cleavage at the H157-S158 bond is accelerated for the Alzheimer's disease-associated H157Y variant EMBO Mol Med, 2017-10-01;0(0):. 2017-10-01 [PMID: 28855301] (Western Blot, Mouse)
• G Kleinberge, M Brendel, E Mracsko, B Wefers, L Groeneweg, X Xiang, C Focke, M Deu beta ing, M Suárez-Cal, F Mazaheri, S Parhizkar, N Pettkus, W Wurst, R Feederle, P Bartenstei, T Mueggler, T Arzberger, I Knuesel, A Rominger, C Haass The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism EMBO J., 2017-05-30;0(0):. 2017-05-30 [PMID: 28559417] (ELISA Development, Mouse)
• M Brendel, G Kleinberge, F Probst, A Jaworska, F Overhoff, T Blume, NL Albert, J Carlsen, S Lindner, FJ Gildehaus, L Ozmen, M Suárez-Cal, P Bartenstei, K Baumann, M Ewers, J Herms, C Haass, A Rominger Increase of TREM2 during Aging of an Alzheimer's Disease Mouse Model Is Paralleled by Microglial Activation and Amyloidosis Front Aging Neurosci, 2017-01-31;9(0):8. 2017-01-31 [PMID: 28197095] (ELISA Development (Detection), Mouse)
• Sandro Alves, Thibaut Marais, Maria-Grazia Biferi, Denis Furling, Martina Marinello, Khalid El Hachimi et al. Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteins Molecular Neurodegeneration 12/1/2016 [PMID: 27465358]
• Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H Osteoprotection by semaphorin 3A. Nature, 2012-05-03;485(7396):69-74. 2012-05-03 [PMID: 22522930] (Western Blot, Mouse)

Review for TREM2 Antibody (MAB1729) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Manjinder Cheema WB Mouse 02/24/2020

Summary

• Application: Western Blot
• Sample Tested: Adult brain,Adult kidney,Liver,Mouse Spleen
• Species: Mouse
• Lot: KCS032001

Comments

• Comments: Used at 1:100 dilution and tested twice with the same results.

Bioinformatics

• Gene Symbol: TREM2
• Uniprot:
  • Q99NH8(Mouse)