TRANCE/TNFSF11/RANK L Antibody - BSA Free

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140] - A. 50 ug rat lung extract 10 % SDS-PAGE RANKL antibody [C2C3], C-term dilution: 1:1000

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140] - Paraffin-embedded human breast carcinoma. RANKL antibody [C2C3], C-term diluted at 1:500.

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NBP1-31140] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE RANKL (CD254) antibody diluted at 1:1000

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, MA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRANCE/TNFSF11/RANK L Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRANCE/TNFSF11/RANK L. The exact sequence is proprietary.
• Localization: Isoform 1: Cell membrane, Single-pass type II membrane protein, Isoform 3: Cell membrane, Single-pass type II membrane protein, Isoform 2: Cytoplasm, Tumor necrosis factor ligand superfamily member 11, soluble form: Secreted
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFSF11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Microarray
  • Western Blot 1:500-1:3000
• Application Notes: Use in microarray reported in scientific literature (PMID: 28955722).
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody - BSA Free

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TRANCE/TNFSF11/RANK L Antibody (NBP1-31140)(1)

Publication using NBP1-31140

• Acharya KD, Nettles SA, Sellers KJ et al. The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent. eNeuro 2017-09-20 [PMID: 28955722] (MiAr, Mouse)

Bioinformatics

• Gene Symbol: TNFSF11