TRANCE/TNFSF11/RANK L Antibody - BSA Free

Western Blot: TRANCE/TNFSF11/RANK L Antibody [NB100-56392] - Analysis of CD254. A. CD254 recombinant protein fragment. Note: this is not a full-length protein. B. LNCaP lysate using CD254 antibody at 0.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TRANCE/TNFSF11/RANK L Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRANCE/TNFSF11/RANK L Antibody - BSA Free (NB100-56392) is a polyclonal antibody validated for use in IHC and WB. Anti-TRANCE/TNFSF11/RANK L Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 161-177 of mouse CD254 (AQPFAHLTINAASIPSG) was used as immunogen for this antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFSF11
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry reported in scientific literature (PMID 30082761)
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody - BSA Free

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis. Mouse Trance/RANKL is a 316 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (NB100-56392)(1)

Publication using NB100-56392

• Li , Wang C, Li Z et al. Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts Cell Death Dis 2018-08-12 [PMID: 30082761] (IF/IHC, WB, Human)

Bioinformatics

• Gene Symbol: TNFSF11
• Entrez:
  • 21943(Mouse)
  • 117516(Rat)
• Uniprot:
  • NP_003692()