TRAILR3/TNFRSF10C Antibody - BSA Free

Western Blot: TRAILR3/TNFRSF10C Antibody [NBP1-76881] - Analysis of 20 ug of whole cell lysate from HeLa (Lane 1) and 293 (Lane 2) cells with anti-TRAIL-R3 at 1 ug/ml dilution. Image submitted by a verified customer review.

Western Blot: TRAILR3/TNFRSF10C Antibody [NBP1-76881] - HeLa whole cell lysate with TRAIL-R3 antibody (ED) at 1:500 dilution.

Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody - BSA Free [NBP1-76881] - Immunofluorescence of TRAILR3/TNFRSF10C in Hela cells with TRAILR3/TNFRSF10C antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody - BSA Free [NBP1-76881] - Immunocytochemistry of TRAILR3/TNFRSF10C in HeLa cells with TRAILR3/TNFRSF10C antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRAILR3/TNFRSF10C Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to amino acids in a extracellular domain of human DcR1 precursor. The immunogen is located within amino acids 140 - 190 of DcR1. Amino Acid Squence: CVEEFGANATVETPAAEET
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFRSF10C
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 0.5 ug/ml
• Application Notes: An approximate 65 kDa band can be detected in Western Blot. TRAILR3/TNFRSF10C antibody validated for WB from a verified customer review.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TRAILR3/TNFRSF10C Protein (NBP1-76881PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for TRAILR3/TNFRSF10C Antibody - BSA Free

• Antagonist decoy receptor for TRAIL/Apo-2L
• CD263 antigen
• CD263
• cytotoxic TRAIL receptor-3
• DcR1
• DCR1MGC149502
• Decoy receptor 1
• Decoy TRAIL receptor without death domain
• LITMGC149501
• Lymphocyte inhibitor of TRAIL
• TNFRSF10C
• TRAIL R3
• TRAIL receptor 3
• TRAIL receptor without an intracellular domain
• TRAILR3
• TRAIL-R3
• TRAILR3TNF-related apoptosis-inducing ligand receptor 3
• TRIDDCR1-TNFR
• tumor necrosis factor receptor superfamily member 10C
• tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAILR3/TNFRSF10C Antibody (NBP1-76881)(4)

Publications using NBP1-76881

• Nagane M, Shimizu S, Mori E et al. Predominant antitumor effects by fully human anti-TRAIL-receptor 2 (DR5) monoclonal antibodies in human glioma cells in vitro and in vivo. Neuro Oncol. 2010-07-01 [PMID: 20511188] (WB, Human)
  
  Details:
  Products cited: 1. DcR2 (IMG-121-1): WB (human glioma cell lines), Fig 4B. 2. DcR1 (IMG-245-1): WB (human glioma cell lines), Fig 4B.
• Taniguchi H, Yoshida T, Horinaka M et al. Baicalein overcomes tumor necrosis factor-related apoptosis-inducing ligand resistance via two different cell-specific pathways in cancer cells but not in normal cells. Cancer Res. 2008-11-01 [PMID: 18974136]
  
  Details:
  Products cited: 1. IMG-121 (DcR2): WB (human colon carcinoma SW480 cell line), Fig 3. 2. IMG-245 (DcR1): WB (human colon carcinoma SW480 cell line), Fig 3.
• Kawakubo T, Okamoto K, Iwata J et al. Cathepsin E prevents tumor growth and metastasis by catalyzing the proteolytic release of soluble TRAIL from tumor cell surface. Cancer Res. 2007-11-15 [PMID: 18006832] (WB)
  
  Details:
  Products cited: 1. IMG-121 (DcR2): WB (human prostate carcinoma cell lines), Fig. 4B. 2. IMG-245 (DcR1): WB (human prostate carcinoma cell lines), Fig. 4B.
• Jin F, Liu X, Zhou Z et al. Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells. Cancer Res. 2005-07-15 [PMID: 16024638] (WB)
  
  Details:
  IMGENEX antibodies cited for WB: 1. Caspase-3 mAb, clone 31A1067 (IMG-144A): Fig 3C, DU145 prostate adenocarcinoma cells. 2. DcR1 pAb (IMG-245-1/IMG-245-2): Fig 6C, DcR1 overexpressing DU145 cells. Note: The specificity of the DcR1 pAb was validated in Dc

Bioinformatics

• Gene Symbol: TNFRSF10C
• Uniprot:
  • AF012536(Human)