TRAILR1/TNFRSF10A Antibody [Unconjugated]

Images

 
Western blot shows lysates of TF‑1 human erythroleukemic cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL R1/TNFRSF10A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF347) followed ...read more
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R1/TNFRSF10A Fc Chimera (Catalog # ...read more
TRAIL R1/TNFRSF10A was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human TRAIL R1/TNFRSF10A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF347) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

TRAILR1/TNFRSF10A Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRAIL R1/TNFRSF10A
Ala24-Asn239
Accession # AAC51226
Specificity
Detects human TRAIL R1/TNFRSF10A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) TRAIL R2 is observed and less than 1% cross-reactivity with rhTRAIL R3 and rhTRAIL R4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF10A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.02-0.055 ug/mL
Control
TRAILR1/TNFRSF10A Knockout HeLa Cell Lysate
TRAILR1/TNFRSF10A Knockout HeLa Cell Lysate
Publications
Read Publications using
AF347 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAILR1/TNFRSF10A Antibody [Unconjugated]

  • APO2
  • CD261 antigen
  • CD261
  • cytotoxic TRAIL receptor
  • Death receptor 4
  • DR4 TRAIL receptor 1
  • DR4
  • TNF-related apoptosis inducing ligand receptor 1
  • TNF-related apoptosis-inducing ligand receptor 1
  • TNFRSF10A
  • TRAIL R1
  • TRAILR1
  • TRAIL-R1
  • TRAILR-1
  • TRAILR1MGC9365
  • tumor necrosis factor receptor superfamily member 10A
  • tumor necrosis factor receptor superfamily, member 10a

Background

Human TRAIL R1, also called DR4, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL RI is referred to as TNFRSF10A. TRAIL R1 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among the TNF receptor family proteins, TRAIL R1 is most closely related to TRAIL R2/DR5, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R1/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R1, an additional TRAIL R2/DR5, which tranduces apoptosis signal, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

  1. Pan, G. et al. (1997) Science 276:111.
  2. Golstein, P. (1997) Curr. Biol. 7:R750.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAILR1/TNFRSF10A Antibody (AF347)(31)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Flow Cytometry, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(10)
IHC-Fr
(2)
IHC-P
(2)
Immunoprecipitation
(1)
Neutralization
(5)
Western Blot
(11)
All Applications
Filter By Species
Human
(31)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using AF347 Applications Species
S Serrano-Sa, C Palacios, D Delgado-Be, L López-Jimé, A Garcia-Dia, Y Soto-Serra, JI Casal, RA Bartolomé, JL Fernández-, A López-Riva, FJ Oliver PIM kinases mediate resistance of glioblastoma cells to TRAIL by a p62/SQSTM1-dependent mechanism Cell Death Dis, 2019;10(2):51. 2019 [PMID: 30718520] (Western Blot, Human) Western Blot Human
Y Shlyakhtin, V Pavet, H Gronemeyer Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells Cell Death Dis, 2017;8(8):e3025. 2017 [PMID: 29048428] (Immunoprecipitation, Human) Immunoprecipitation Human
V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma Cancer Med, 2017;0(0):. 2017 [PMID: 28799237] (Western Blot, Human) Western Blot Human
C Liang, Y Xu, G Li, T Zhao, F Xia, G Li, D Zhang, J Wu Downregulation of DcR3 sensitizes hepatocellular carcinoma cells to TRAIL-induced apoptosis Onco Targets Ther, 2017;10(0):417-428. 2017 [PMID: 28176915] (Neutralization, Human) Neutralization Human
Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs). Anticancer Res, 2014;34(12):7007-20. 2014 [PMID: 25503127] (Flow Cytometry, Human) Flow Cytometry Human
Elias A, Siegelin MD, Steinmuller A, von Deimling A, Lass U, Korn B, Mueller W Epigenetic silencing of death receptor 4 mediates tumor necrosis factor-related apoptosis-inducing ligand resistance in gliomas. Clin. Cancer Res., 2009;15(17):5457-65. 2009 [PMID: 19706813] (Neutralization, Human) Neutralization Human
Park SJ, Sohn HY, Yoon J, Park SI Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells. Cell. Signal., 2009;21(10):1495-503. 2009 [PMID: 19470406] (Flow Cytometry, Human) Flow Cytometry Human
Park SJ, Kim MJ, Kim HB, Sohn HY, Bae JH, Kang CD, Kim SH Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway. Biochem. Pharmacol., 2009;77(8):1328-36. 2009 [PMID: 19426671] (Western Blot, Human) Western Blot Human
Siegelin MD, Gaiser T, Habel A, Siegelin Y Myricetin sensitizes malignant glioma cells to TRAIL-mediated apoptosis by down-regulation of the short isoform of FLIP and bcl-2. Cancer Lett., 2009;283(2):230-8. 2009 [PMID: 19398149] (Western Blot, Human) Western Blot Human
Festuccia C, Gravina GL, D'Alessandro AM, Millimaggi D, Di Rocco C, Dolo V, Ricevuto E, Vicentini C, Bologna M Downmodulation of dimethyl transferase activity enhances tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in prostate cancer cells. Int. J. Oncol., 2008;33(2):381-8. 2008 [PMID: 18636160] (Western Blot, Human) Western Blot Human
Show All 31 Publications.

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Control Lysate(s)

Secondary Antibodies

 

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Biotin BAF347

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Bioinformatics

Gene Symbol TNFRSF10A
Uniprot