TRAF-3 Antibody

Western Blot: TRAF-3 Antibody [NB100-56654] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: TRAF-3 Antibody [NB100-56654] - Analysis of human pancreas using TRAF3 antibody at 1:50.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRAF-3 Antibody Summary

• Immunogen: A peptide corresponding to amino acids between 300 and 350 of human TRAF3 was used as immunogen for this antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1 - 3 ug/ml
• Application Notes: A 67 kDa band is observed.
• Control:

  TRAF-3 Overexpression Lysate
  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS 0.5%BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TRAF-3 Antibody

• CAP1
• CAP-1CD40 receptor associated factor 1
• CD40 associated protein 1
• CD40 binding protein
• CD40 receptor-associated factor 1
• CD40-binding protein
• CD40bp
• CRAF1
• CRAF1CAP1
• LAP1
• LAP1CD40BP
• LMP1-associated protein 1
• TNF receptor-associated factor 3
• TRAF3
• TRAF-3

Background

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. TRAF3 binds only a single site, which contains the sequence PEQET, whereas TRAF1 and TRAF2 are capable of binding to either the PEQET site or an additional downstream domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-3 Antibody (NB100-56654)(1)

Publication using NB100-56654

• Clijsters L, Hoencamp C, Calis JJA et al. Chandipura virus changes cellular miRNome in human microglial cells J. Med. Virol. 2019-04-24 [PMID: 31017674] (WB, Human)

Bioinformatics

• Gene Symbol: TRAF3
• Entrez:
  • 7187(Human)
  • 22031(Mouse)
• Uniprot:
  • NP_663778()