| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56176SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3278-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3278-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56175SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86639-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76642SS | |||
![Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NB100-56176-img0001.jpg "Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.")
![Western Blot: TRAF-3 Antibody [NB100-56654] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Western-Blot-NB100-56654-img0006.jpg "Western Blot: TRAF-3 Antibody [NB100-56654] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: TRAF-3 Antibody [NB100-56654] - Analysis of human pancreas using TRAF3 antibody at 1:50.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NB100-56654-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAF-3 Antibody [NBP1-86639] - Staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NBP1-86639-img0001.jpg "Immunohistochemistry-Paraffin: TRAF-3 Antibody [NBP1-86639] - Staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.")
![Western Blot: TRAF-3 Antibody [NBP1-76642] - HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Western-Blot-NBP1-76642-img0001.jpg "Western Blot: TRAF-3 Antibody [NBP1-76642] - HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: TRAF-3 Antibody - BSA Free [NBP1-76642] - Immunocytochemistry of TRAF-3 in HeLa cells with TRAF-3 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76642_rabbit-polyclonal-traf-3-antibody-271120242016971.jpg)
![Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042] - Western blot analysis of TRAF-3 on different lysates with Rabbit anti-TRAF-3 antibody (NBP3-33042) at 1/1,000 dilution. Lane 1: TF-1 cell lysate (20 ug/Lane) Lane 2: THP-1 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 6: RAW264.7 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Mouse brain tissue lysate (40 ug/Lane) Lane 9: Rat spleen tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 64 kDa Observed band size: 50 kDa Exposure time: 1 minute 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33042) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33042_rabbit-traf-3-mab-psh01-30-288202412493419.jpg "Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042] - Western blot analysis of TRAF-3 on different lysates with Rabbit anti-TRAF-3 antibody (NBP3-33042) at 1/1,000 dilution. Lane 1: TF-1 cell lysate (20 ug/Lane) Lane 2: THP-1 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 6: RAW264.7 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Mouse brain tissue lysate (40 ug/Lane) Lane 9: Rat spleen tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 64 kDa Observed band size: 50 kDa Exposure time: 1 minute 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33042) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")