Rictor Antibody

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Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ...read more
Genetic Strategies: Knockdown Validated: Rictor Antibody [NB100-612] - SERCA2A expression is regulated by mTORC2. Rictor silencing was induced by cell transfection with rictor siRNA and then, cells were ...read more
Independent Antibodies: Immunoprecipitation: Rictor Antibody [NB100-612] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more
Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions & rictor phosphorylation at Ser1235. Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin, then stimulated with 10 nM IGF-1 in ...read more
Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Biological Strategies: Western Blot: Rictor Antibody [NB100-612] - Detection of RICTOR on SKOV3 whole cell lysate, treated with PEITC. Image courtesy of product review by Parul Gupta (Texas Tech University Health ...read more
Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. ...read more
Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions and rictor phosphorylation at Ser1235.Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin and then stimulated with 10 nM IGF-1 in ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Rictor Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1650 and the C-terminus (residue 1708) of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RICTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 25378594). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-612 in the following applications:

Publications
Read Publications using
NB100-612 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rictor Antibody

  • AVO3 homolog
  • AVO3
  • hAVO3
  • KIAA1999DKFZp686B11164
  • mAVO3
  • MGC39830
  • PIA
  • Pianissimo
  • rapamycin-insensitive companion of mTOR
  • Rictor
  • RPTOR independent companion of MTOR, complex 2
  • TORC2-specific protein AVO3

Background

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-612
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, KD

Publications for Rictor Antibody (NB100-612)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
WB
(7)
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Filter By Species
Human
(7)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB100-612 Applications Species
Gerlach BD, Ampomah PB, Yurdagul A Et al. Efferocytosis induces macrophage proliferation to help resolve tissue injury Cell metabolism Nov 10 2021 [PMID: 34784501]
Cameron AJM, Veeriah S, Marshall JJT et al. Uncoupling TORC2 from AGC kinases inhibits tumour growth Oncotarget. 2017 Oct 17 [PMID: 29156676] (WB, Human) WB Human
Liu WL, Yang HC, Hsu CS et al. Pegylated IFN-a suppresses hepatitis C virus by promoting the DAPK-mTOR pathway. Proc. Natl. Acad. Sci. U.S.A. Dec 20 2016 [PMID: 27930338] (WB, Human) WB Human
Tsai JS, Chao CH, Lin LY. Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability. PLoS ONE. 2016 Jan 12 [PMID: 26751215] (WB, Human) WB Human
Kusch A, Schmidt M, Gurgen D et al. 17-Beta-Estradiol Regulates mTORC2 Sensitivity to Rapamycin in Adaptive Cardiac Remodeling PLoS ONE 2015 Apr 17 [PMID: 25880554] (WB, Mouse) WB Mouse
Sen S, Hassane DC, Corbett C et al. Novel mTOR inhibitory activity of ciclopirox enhances parthenolide anti-leukemia activity. Exp Hematol 2013 May 6 [PMID: 23660068] (WB, Human) WB Human
Ko J ALTERNATIVE POLYADENYLATION MODULATES EXPRESSION OF PRO-FIBROTIC PROTEINS AND CONTRIBUTES TO LUNG FIBROSIS Thesis Jan 1 2020 (WB, Human) WB Human
Smrz D, Cruse G, Beaven Ma et al. Rictor negatively Regulates High-Affinity Receptors for IgE-Induced Mast Cell Degranulation J. Immunol. 2014 Nov 05 [PMID: 25378594] (ICC/IF, Human)

Details:
Rictor antibody used for ICC-IF on LAD2 human mast cell line (FIGURE 1).
ICC/IF Human
Ziegler ME, Jin YP, Young SH et al. HLA class I-mediated stress fiber formation requires ERK1/2 activation in the absence of an increase in intracellular Ca2 in human aortic endothelial cells Am J Physiol Cell Physiol 2012 Oct [PMID: 22914643] (WB, IP, Human) WB, IP Human
Show All 21 Publications.

Reviews for Rictor Antibody (NB100-612) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-612:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Rictor NB100-612
Enlarge
5
reviewed by:
Parul Gupta
WB Human 08/26/2012
View

Summary

ApplicationWestern Blot
Sample TestedSKOV3 whole cell lysate
SpeciesHuman
  5
reviewed by:
Jia-Shiuan Tsai
WB Human 12/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Product General Protocols

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ICC/IF Video Protocol

FAQs for Rictor Antibody (NB100-612) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Rictor Antibody (NB100-612)

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Research Areas for Rictor Antibody (NB100-612)

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Blogs on Rictor.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Parul Gupta
08/26/2012
Application: WB
Species: Human

 
Jia-Shiuan Tsai
12/09/2011
Application: WB
Species: Human

Bioinformatics

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