Rictor Antibody

Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Knockdown Validated: Rictor Antibody [NB100-612] - SERCA2A expression is regulated by mTORC2. Rictor silencing was induced by cell transfection with rictor siRNA and then, cells were stimulated with IGF-1 for 24 hours in presence or absence of E2. mTORC2 downregulation was confirmed by abolished Akt-pS473 and resulted in decreased SERCA2A protein expression. Akt-pT308, mTOR and raptor were not negatively affected by rictor silencing. Pictured are representative western blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY licence.

Immunoprecipitation: Rictor Antibody [NB100-612] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody NB100-612 (lot NB100-612-4) used for IP at 3 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot NB100-612-3) and rabbit anti-Rictor antibody NB100-611. For blotting immunoprecipitated Rictor, NB100-612 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions & rictor phosphorylation at Ser1235. Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin, then stimulated with 10 nM IGF-1 in presence/absence of 10 nM E2 for 24 h. Western blots for GSK3beta-pS9, GSK3beta, rictor pS1235 and rictor. At least 3 independent experiments indicate that rictor phosphorylation at S1235 by GSK-3beta downregulates mTORC2 activity. IGF-1 induced strong phosphorylation of GSK-3beta at Ser9 in the absence and presence of E2. Rapamycin pretreatment reduced phosphorylation in E2 co-treated cardiomyocytes, indicating increased activity of GSK-3beta, which was not associated with increased phosphorylation of rictor at S1235. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: Rictor Antibody [NB100-612] - Detection of RICTOR on SKOV3 whole cell lysate, treated with PEITC. Image courtesy of product review by Parul Gupta (Texas Tech University Health Sciences Center).

Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions and rictor phosphorylation at Ser1235.Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin and then stimulated with 10 nM IGF-1 in presence or absence of 10 nM E2 for 24 h. A, shown are representative western blots for mTOR, rictor and raptor; quantitative analysis was performed with mean +/- SEM of fold stimulation by IGF-1 of at least 3 independently performed experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY licence.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Rictor Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1650 and the C-terminus (residue 1708) of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RICTOR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID 25378594). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 192 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rictor Antibody

• AVO3 homolog
• AVO3
• hAVO3
• KIAA1999DKFZp686B11164
• mAVO3
• MGC39830
• PIA
• Pianissimo
• rapamycin-insensitive companion of mTOR
• Rictor
• RPTOR independent companion of MTOR, complex 2
• TORC2-specific protein AVO3

Background

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rictor Antibody (NB100-612)(21)

Publications using NB100-612

• Gerlach BD, Ampomah PB, Yurdagul A Et al. Efferocytosis induces macrophage proliferation to help resolve tissue injury Cell metabolism Nov 10 2021 [PMID: 34784501]
• Cameron AJM, Veeriah S, Marshall JJT et al. Uncoupling TORC2 from AGC kinases inhibits tumour growth Oncotarget. 2017 Oct 17 [PMID: 29156676] (WB, Human)
• Liu WL, Yang HC, Hsu CS et al. Pegylated IFN-a suppresses hepatitis C virus by promoting the DAPK-mTOR pathway. Proc. Natl. Acad. Sci. U.S.A. Dec 20 2016 [PMID: 27930338] (WB, Human)
• Tsai JS, Chao CH, Lin LY. Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability. PLoS ONE. 2016 Jan 12 [PMID: 26751215] (WB, Human)
• Kusch A, Schmidt M, Gurgen D et al. 17-Beta-Estradiol Regulates mTORC2 Sensitivity to Rapamycin in Adaptive Cardiac Remodeling PLoS ONE 2015 Apr 17 [PMID: 25880554] (WB, Mouse)
• Sen S, Hassane DC, Corbett C et al. Novel mTOR inhibitory activity of ciclopirox enhances parthenolide anti-leukemia activity. Exp Hematol 2013 May 6 [PMID: 23660068] (WB, Human)
• Ko J ALTERNATIVE POLYADENYLATION MODULATES EXPRESSION OF PRO-FIBROTIC PROTEINS AND CONTRIBUTES TO LUNG FIBROSIS Thesis Jan 1 2020 (WB, Human)
• Smrz D, Cruse G, Beaven Ma et al. Rictor negatively Regulates High-Affinity Receptors for IgE-Induced Mast Cell Degranulation J. Immunol. 2014 Nov 05 [PMID: 25378594] (ICC/IF, Human)
  
  Details:
  Rictor antibody used for ICC-IF on LAD2 human mast cell line (FIGURE 1).
• Ziegler ME, Jin YP, Young SH et al. HLA class I-mediated stress fiber formation requires ERK1/2 activation in the absence of an increase in intracellular Ca2 in human aortic endothelial cells Am J Physiol Cell Physiol 2012 Oct [PMID: 22914643] (WB, IP, Human)
• Shanmugasundaram K, Block K, Nayak BK et al. PI3K regulation of the SKP-2/p27 axis through mTORC2. Oncogene 2012 Jun [PMID: 22733130]
• Gulhati P, Bowen KA, Liu J et al. mTORC1 and mTORC2 regulate EMT, motility, and metastasis of colorectal cancer via RhoA and Rac1 signaling pathways. Cancer Res 2011 May [PMID: 21430067]
• Siegel N, Rosner M, Unbekandt M et al. Contribution of human amniotic fluid stem cells to renal tissue formation depends on mTOR. Hum Mol Genet 2010 Sep [PMID: 20542987]
• Gulhati P, Cai Q, Li J et al. Targeted inhibition of mammalian target of rapamycin signaling inhibits tumorigenesis of colorectal cancer. Clin Cancer Res 2009 Dec [PMID: 19934294]
• Mills JR, Hippo Y, Robert F et al. mTORC1 promotes survival through translational control of Mcl-1. Proc Natl Acad Sci U S A 2008 Aug [PMID: 18664580]
• Hagan GN, Lin Y, Magnuson MA et al. A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes. Mol Cell Biol 2008 Jul [PMID: 18426911]
• McDonald PC, Oloumi A, Mills J et al. Rictor and integrin-linked kinase interact and regulate Akt phosphorylation and cancer cell survival. Cancer Res 2008 Mar [PMID: 18339839]
• Jindra, P T, Y-P Jin et al. HLA Class I Antibody-Mediated Endothelial Cell Proliferation via the mTOR Pathway. J Immunol 180: 2357-2366. 2008 [PMID: 18250445]
• Vary TC, Deiter G, Lynch CJ et al. Rapamycin limits formation of active eukaryotic initiation factor 4F complex following meal feeding in rat hearts. J Nutr 2007 Aug [PMID: 17634255]
• Yang Q, Inoki K, Ikenoue T et al. Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity. Genes Dev 2006 Oct [PMID: 17043309]
• Takahara T, Hara K, Yonezawa K et al. Nutrient-dependent multimerization of the mammalian target of rapamycin through the N-terminal HEAT repeat region. J Biol Chem 2006 Sep [PMID: 16870609]

Reviews for Rictor Antibody (NB100-612) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Parul Gupta WB Human 08/26/2012

Summary

• Application: Western Blot
• Sample Tested: SKOV3 whole cell lysate
• Species: Human

reviewed by: Jia-Shiuan Tsai WB Human 12/09/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

Bioinformatics

• Gene Symbol: RICTOR
• Entrez:
  • 253260(Human)
  • 78757(Mouse)
• Uniprot:
  • Q6R327()