Tollip Antibody (SB40a) - Azide and BSA Free

Western Blot: Tollip Antibody (SB40a) [NBP1-28621] - 2 ug Mouse Anti-Human Tollip-UNLB on Jurkat cell lysate with Goat Anti-Mouse IgG2a, human adsorbed-HRP

Flow Cytometry: Tollip Antibody (SB40a) [NBP1-28621] - An intracellular stain was performed on HeLa cells with Tollip Antibody (SB40a) NBP1-28621PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: SB40a
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Tollip Antibody (SB40a) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Tollip Antibody (SB40a) - Azide and BSA Free (NBP1-28621) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant Tollip
• Specificity: Human Tollip; Does not react to TLR2, TLR4, TLR6, TLR7, TLR8 and TLR10 recombinant proteins
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOLLIP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Tollip Antibody (SB40a) - Azide and BSA Free

• adapter protein
• FLJ33531
• IL-1RAcPIP
• toll interacting protein
• toll-interacting protein
• Tollip

Background

Tollip, Toll-interacting protein, is a 30 kDa adaptor protein that is a component of the signaling pathway of IL-1 and Toll-like receptors. Tollip forms a complex with IRAK (IL-1 receptor associated kinase) and inhibits IL-1-induced signaling by blocking IRAK phosphorylation. The co-recruitment of MyD88 triggers IRAK autophosphorylation, which in turn leads to the rapid dissociation of IRAK and Tollip. Tollip is also an important signaling component of the TLR2 and TLR4 pathways by playing an inhibitory role in TLRmediated cell activation. Tollip also associates with Tom1 (target of Myb1) forming a complex which regulates endosomal trafficking of ubiquitinated proteins. (1-6)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tollip Antibody (NBP1-28621)(1)

Publication using NBP1-28621

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: TOLLIP