TNF-alpha Antibody [Unconjugated]

Images

 
TNF‑ alpha was detected in immersion fixed rat splenocytes using Goat Anti-Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody(Catalog # AF-510-NA) at 15 µg/mL for 3 hours at room temperature. ...read more
Recombinant Rat TNF‑ alpha (Catalog # 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

TNF-alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat TNF-alpha
Leu80-Leu235
Accession # P16599
Specificity
Detects rat TNF‑ alpha in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant porcine TNF-alpha is observed and less than 1% cross-reactivity with recombinant human (rh) TNF-beta, rhTNF sRI, and rhTNF sRII is observed. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine TNF-alpha, recombinant feline TNF-alpha, and recombinant rhesus macaque TNF-alpha is observed. Neutralizes the biological activity of rat TNF-alpha. This antibody will also neutralize the biological activity of murine TNF-alpha, but weak neutralization of rhTNF-alpha and no neutralization of rhTNF-beta was observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNF
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.15-0.75 ug/mL
Publications
Read Publications using
AF-510-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody [Unconjugated]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69% - 76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

  1. Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Estler, H.C. et al. (1992) Biol. Chem. Hoppe-Seyler 373:271.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  9. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
  10. Romanatto, T. et al. (2007) Peptides 28:1050.
  11. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF-510-NA
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Applications: WB, B/N, ICC/IF

Publications for TNF-alpha Antibody (AF-510-NA)(24)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(2)
IHC
(3)
IHC-Fr
(3)
IHC-P
(1)
Neutralization
(9)
Western Blot
(5)
All Applications
Filter By Species
Rat
(24)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF-510-NA Applications Species
ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBP? in Alzheimer&#039;s Disease Cell Rep, 2019;28(3):655-669.e5. 2019 [PMID: 31315045] (Neutralization, Rat) Neutralization Rat
HC Lai, CH Lu, CS Wong, BF Lin, SM Chan, CY Kuo, ZF Wu Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection J Chin Med Assoc, 2018;0(0):. 2018 [PMID: 30139671] (Rat) Rat
YW Zhao, YQ Pan, MM Tang, WJ Lin Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by Neuroinflammation Front Pharmacol, 2018;9(0):511. 2018 [PMID: 29867510] (Western Blot, Rat) Western Blot Rat
L Ma, Y Jiang, Y Dong, J Gao, B Du, D Liu Anti-TNF-alpha antibody attenuates subarachnoid hemorrhage-induced apoptosis in the hypothalamus by inhibiting the activation of Erk Neuropsychiatr Dis Treat, 2018;14(0):525-536. 2018 [PMID: 29497296] (Western Blot, Rat) Western Blot Rat
SA Chong, S Balosso, C Vandenplas, G Szczesny, E Hanon, K Claes, X Van Damme, B Danis, J Van Eyll, C Wolff, A Vezzani, RM Kaminski, I Niespodzia Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model Neurotherapeutics, 2018;0(0):. 2018 [PMID: 29464573] (Neutralization, Rat) Neutralization Rat
E DeVallance, KW Branyan, K Lemaster, IM Olfert, DM Smith, EE Pistilli, JC Frisbee, PD Chantler Aortic dysfunction in metabolic syndrome mediated by perivascular adipose tissue TNF? and NOX2 dependent pathway Exp. Physiol., 2018;0(0):. 2018 [PMID: 29349831] (Neutralization, Rat) Neutralization Rat
ZL Wang, LY Yang, HH Chen, HH Lin, YT Tsai, WJ Huang Effects of TNF-? on penile structure alteration in rats with hyperprolactinemia PLoS ONE, 2017;12(8):e0181952. 2017 [PMID: 28763467] (Neutralization, Rat) Neutralization Rat
JS Medel-Matu, A Reynolds, D Shin, R Sankar, A Mazarati Regulation of kindling epileptogenesis by hippocampal Toll-like receptors 2 Epilepsia, 2017;0(0):. 2017 [PMID: 28632301] (IHC, Rat) IHC Rat
Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. PLoS ONE, 2014;9(8):e106331. 2014 [PMID: 25170959] (IHC-Fr, Rat) IHC-Fr Rat
Masson G, Costa T, Yshii L, Fernandes D, Soares P, Laurindo F, Scavone C, Michelini L Time-dependent effects of training on cardiovascular control in spontaneously hypertensive rats: role for brain oxidative stress and inflammation and baroreflex sensitivity. PLoS ONE, 2014;9(5):e94927. 2014 [PMID: 24788542] (IHC-Fr, Rat) IHC-Fr Rat
Show All 24 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TNF-alpha Antibody (AF-510-NA). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF510

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Pattern recognition receptors (PRR) expression and function in endothelial cells
By Victoria OsinskiWhat are Pattern recognition receptors?Pattern recognition receptors (PRRs) recognize and respond to Pattern- and Damage-associated molecular patterns (PAMP and DAMPs) in a number of different c...  Read full blog post.

COVID-19-Related Neurological Problems: Uncovering the Mechanisms
By Jamshed Arslan, Pharm D, PhDThere is good news and bad news related to COVID-19, a disease caused by SARS-COV-2 infection. Good news first: the vast majority of otherwise healthy individuals remain asymptomatic...  Read full blog post.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Bioinformatics

Gene Symbol TNF
Uniprot