TNF-alpha Antibody (52B83)

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Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rb, ShSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
52B83
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

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Catalog# & Conjugate Size Price
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TNF-alpha Antibody (52B83) Summary

Immunogen
The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
Localization
Type 2 membrane protein or soluble form derived from the membrane by proteolytic cleavage.
Specificity
This detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. Non-specific background staining is obesrved in connective tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10-1:100
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha (17.4 kDa) is not biologically active.
Reviewed Applications
Read 1 Review rated 3
using
NB600-1422 in the following applications:

Publications
Read Publications using
NB600-1422 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27390101). Expected cross reactivity based on sequence identity: Guinea pig, Rhesus Monkey. Sheep data from customer review. Rabbit reactivity reported in scientific literature (PMID: 28114570).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS with 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for TNF-alpha Antibody (52B83)

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

umor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-1422
Species: Hu, Mu, Rb, Sh
Applications: WB, Flow, IHC, IHC-Fr, IHC-P

Publications for TNF-alpha Antibody (NB600-1422)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 2 applications: ELISA, IHC.


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ELISA
(1)
IHC
(4)
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Human
(1)
Mouse
(1)
Rabbit
(2)
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Review for TNF-alpha Antibody (NB600-1422) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB600-1422:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TNF-alpha NB600-1422
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reviewed by:
Anonymous
IHC-P Other 08/11/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedsheep tonsil
SpeciesOther
CommentsDoes have some background staining in connective tissue and other cell types. Best conditions were with no antigen retrieval.

Primary Anitbody

Dilution Ratio1:150

Details

Fixation DetailsFFPE, no antigen retrieval

Comments

CommentsDoes have some background staining in connective tissue and other cell types. Best conditions were with no antigen retrieval.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNF-alpha Antibody (NB600-1422). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

FITC NBP1-51502

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Blogs on TNF-alpha.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews

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1
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Anonymous
08/11/2014
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol TNF
Entrez