TNF-alpha Antibody (103314)

Recombinant Porcine TNF‑ alpha (Catalog # 690‑PT) induces cytotoxicity in the the PK‑15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF‑ alpha (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF‑ alpha Monoclonal Antibody (Catalog # MAB6901). The ND50 is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

• Reactivity: Po
• Applications: B/N
• Clone: 103314
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TNF-alpha Antibody (103314) Summary

• Immunogen: E. coli-derived recombinant porcine TNF-alpha
  Arg78-Leu232
  Accession # P23563
• Specificity: Detects porcine TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 13% cross-reactivity with recombinant human (rh) TNF-alpha is observed and no cross-reactivity with recombinant mouse TNF-alpha, recombinant rat TNF-alpha, rhTNF-beta, rhAPRIL, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTrance, and rhVEGF is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNF
• Purity: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.015-0.06 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody (103314)

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Porcine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 176 aa extracellular domain (ECD) (3). Within the ECD, porcine TNF-alpha shares 69%‑86% aa sequence identity with bovine, canine, cotton rat, equine, feline, human, mouse, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

1. Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. 
2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. 
3. Pauli, U. et al. (1989) Gene 81:185.
4. Tang, P. et al. (1996) Biochemistry 35:8216. 
5. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. 
6. Black, R.A. et al. (1997) Nature 385:729.
7. Moss, M.L. et al. (1997) Nature 385:733.
8. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
9. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
10. Romanatto, T. et al. (2007) Peptides 28:1050.
11. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF-alpha Antibody (MAB6901)(2)

Publications using MAB6901

• C Martin, SD Waghela, S Lokhandwal, A Ambrus, J Bray, C Vuong, V Vinodkumar, PJ Dominowski, S Rai, D Mwangi, DL Foss, W Mwangi Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant PLoS ONE, 2017;12(1):e0170504. 2017 [PMID: 28107431] (Flow Cytometry, Porcine)
• McNulty AL, Moutos FT, Weinberg JB, Guilak F Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin-1 or tumor necrosis factor alpha. Arthritis Rheum., 2007;56(9):3033-42. 2007 [PMID: 17729298] (Neutralization, Porcine)

FAQs for TNF-alpha Antibody (MAB6901). (Showing 1 - 4 of 4 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Bioinformatics

• Gene Symbol: TNF
• Uniprot:
  • P23563()