TLR7 Antibody (533707) [Phycoerythrin]

Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PE-conjugated Monoclonal Antibody (Catalog # IC5875P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Gating strategy for the detection of TLR-7 and -9 in B cell subsets.PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-7 and -9. FSC and SSC were first used to gate out debris (A) and SSC-A and SSC-H was utilized to eliminate duplicates (B). Further gating was done on CD45 and CD19, to target B cells (C). To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-7 and -9 expression on these subsets (E). Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120383), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 533707
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

TLR7 Antibody (533707) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human TLR7
  Met360-Leu516
  Accession # Q9NYK1
• Specificity: Detects human TLR7 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR7 Antibody (533707) [Phycoerythrin]

• TLR7
• toll-like receptor 7

Background

Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR7 Antibody (IC5875P)(10)

Publications using IC5875P

• Shen, P;Serve, S;Wu, P;Liu, X;Dai, Y;Durán-Hernández, N;Nguyen, DTM;Fuchs, M;Maleitzke, T;Reisener, MJ;Dzamukova, M;Nussbaumer, K;Brunner, TM;Li, Y;Holecska, V;Heinz, GA;Heinrich, F;Durek, P;Katsoula, G;Gwinner, C;Jung, T;Zeggini, E;Winkler, T;Mashreghi, MF;Pumberger, M;Perka, C;Löhning, M; NOS inhibition reverses TLR2-induced chondrocyte dysfunction and attenuates age-related osteoarthritis Proceedings of the National Academy of Sciences of the United States of America 2023-07-18 [PMID: 37428931] (Flow Cytometry, Human)
• Ritu M. Ramamurthy, Martin Rodriguez, Hannah C. Ainsworth, Jordan Shields, Diane Meares, Colin Bishop et al. Comparison of different gene addition strategies to modify placental derived-mesenchymal stromal cells to produce FVIII Frontiers in Immunology 12/15/2022 [PMID: 36591257]
• R Serrano, C Coch, C Peters, G Hartmann, D Wesch, D Kabelitz Monocyte-dependent co-stimulation of cytokine induction in human gammadelta T cells by TLR8 RNA ligands Scientific Reports, 2021-07-27;11(1):15231. 2021-07-27 [PMID: 34315922] (Flow Cytometry, Human)
• Marloes van van Splunter, Olaf Perdijk, Henriëtte Fick-Brinkhof, Esther G. Floris-Vollenbroek, Ben Meijer, Sylvia Brugman et al. Plasmacytoid dendritic cell and myeloid dendritic cell function in ageing: A comparison between elderly and young adult women PLOS ONE 12/12/2019 [PMID: 31830086]
• H El Aggan, N Farahat, N El Deeb, A Zeid, A El-Shendid Peripheral blood and hepatic Toll-like receptor 7 expression and interferon lambda 1 levels in chronic hepatitis C: Relation to virus replication and liver injury Microb. Pathog., 2019-03-26;0(0):. 2019-03-26 [PMID: 30926417] (Flow Cytometry, Human)
• Marloes van Splunter, Olaf Perdijk, Henriëtte Fick-Brinkhof, Anouk L. Feitsma, Esther G. Floris-Vollenbroek, Ben Meijer et al. Bovine Lactoferrin Enhances TLR7-Mediated Responses in Plasmacytoid Dendritic Cells in Elderly Women: Results From a Nutritional Intervention Study With Bovine Lactoferrin, GOS and Vitamin D Frontiers in Immunology 11/20/2018 [PMID: 30515164]
• Karlsen M, Hansen T, Nordal H, Brun J, Jonsson R, Appel S Expression of Toll-like receptor -7 and -9 in B cell subsets from patients with primary Sjogren's syndrome. PLoS ONE, 2015-03-19;10(3):e0120383. 2015-03-19 [PMID: 25790192] (IHC-Fr, Human)
• Metcalf T, Cubas R, Ghneim K, Cartwright M, Grevenynghe J, Richner J, Olagnier D, Wilkinson P, Cameron M, Park B, Hiscott J, Diamond M, Wertheimer A, Nikolich-Zugich J, Haddad E Global analyses revealed age-related alterations in innate immune responses after stimulation of pathogen recognition receptors. Aging Cell, 2015-02-27;14(3):421-32. 2015-02-27 [PMID: 25728020] (Flow Cytometry, Human)
• Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages. PLoS Pathog, 2012-09-20;8(9):e1002937. 2012-09-20 [PMID: 23028330] (Flow Cytometry, Human)
• Ma CJ, Ni L, Zhang Y, Zhang CL, Wu XY, Atia AN, Thayer P, Moorman JP, Yao ZQ PD-1 negatively regulates interleukin-12 expression by limiting STAT-1 phosphorylation in monocytes/macrophages during chronic hepatitis C virus infection. Immunology, 2010-11-23;132(3):421-31. 2010-11-23 [PMID: 21091911] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TLR7
• Uniprot:
  • Q9NYK1()