TLR3 Antibody


Western Blot: TLR3 Antibody [NB110-93591] - analysis of TLR3 in human macrophages using anti-TLR3 antibody. Image from verified customer review.
Western Blot: TLR3 Antibody [NB110-93591] - Detection of TLR3 in Daudi whole cell lysates using NB110-93591.

Product Details

Reactivity Hu, Po, Fe, PmSpecies Glossary
Applications WB
1 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

TLR3 Antibody Summary

Synthetic peptide made to a C-terminal portion of the human TLR3 protein (within residues 800-904). [Swiss-Prot# O15455]
Cell membrane; Single-pass type I membrane protein
Predicted Species
Mouse (92%), Rat (92%), Bovine (92%), Equine (92%)
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This TLR3 antibody is useful for Western blot, where a band is seen at ~105 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
105 kDa
Reviewed Applications
Read 1 Review rated 5
NB110-93591 in the following application:

Reactivity Notes

Human, primate, porcine and feline. 92% sequence identity with mouse, rat, equine and bovine

Alternate Names for TLR3 Antibody

  • CD283 antigen
  • CD283
  • toll-like receptor 3


TLR3 (toll-like receptor 3) receptors are a key component of immune system, and represents an intracellular recognition system that specifically respond to RNA viral infections with its unique nucleotide-sensing capability. TLR3 is activated by double-stranded RNAs, and it triggers the production of type 1 IFN as well as inflammatory cytokines/chemokines. TLR3 acts via MYD88/TRAF6 resulting in NFkB activation and cytokine secretion followed by inflammatory response. TLR3 is expressed by epithelial cells, a broad range of antigen-processing cells, tissue dendritic cells, monocytes, mast cells, NK cells etc. TLR3 signaling results in a local cytokine burst which is microenvironment- and cell-specific in nature and leads to local inflammatory response adaptively selected to provide a greatest chance of infection control for affected tissues. TLR3 activation also leads to local production of acute response molecules such as type I IFN, TNF-alpha, IL-12, MCP-1 etc. Defects in TLR3 have been linked to herpes simplex encephalitis type 2 (HSE2) and TLR3 agonists are emerging as promising adjuvants in dendritic cells - based immunotherapy for the treatment of viral and neoplastic diseases.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR3 Antibody (NB110-93591) (0)

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Reviews for TLR3 Antibody (NB110-93591) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-93591:
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Images Ratings Applications Species Date Details
Western Blot TLR3 NB110-93591
reviewed by:
WB Human 12/06/2014


ApplicationWestern Blot
Sample TestedHuman macrophages


Blocking DetailsPBS with 5% non-fat milk

Primary Anitbody

Dilution Ratio1:1000 in PBST(0.05%) with 2% non-fat milk; Overnight, 4oC

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP
Secondary Manufacturer Cat##7074S
Secondary Concentration1:1000


Detection NotesExposure time: 1 min; FujiFilm LAS-1000

Product General Protocols

View specific protocols for TLR3 Antibody (NB110-93591): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR3 Antibody (NB110-93591) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-93591V
FITC Labeled NB110-93591F

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Blogs on TLR3.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol TLR3

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