TLR3 Antibody

Images

 
Western Blot: TLR3 Antibody [NB110-93591] - analysis of TLR3 in human macrophages using anti-TLR3 antibody. Image from verified customer review.
Western Blot: TLR3 Antibody [NB110-93591] - Detection of TLR3 in Daudi whole cell lysates using NB110-93591.

Product Details

Summary
Reactivity Hu, Po, Fe, Pm, Mu, Rt, Bv, EqSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

TLR3 Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the human TLR3 protein (within residues 800-904). [Swiss-Prot# O15455]
Localization
Cell membrane; Single-pass type I membrane protein
Predicted Species
Mouse (92%), Rat (92%), Equine (92%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
This TLR3 antibody is useful for Western blot, where a band is seen at ~105 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Reviewed Applications
Read 1 Review rated 5
using
NB110-93591 in the following application:

Reactivity Notes

Human, primate, porcine and feline. 92% sequence identity with mouse, rat, equine and bovine

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLR3 Antibody

  • CD283 antigen
  • CD283
  • TLR3
  • toll-like receptor 3

Background

TLR3 (toll-like receptor 3) receptors are a key component of immune system, and represents an intracellular recognition system that specifically respond to RNA viral infections with its unique nucleotide-sensing capability. TLR3 is activated by double-stranded RNAs, and it triggers the production of type 1 IFN as well as inflammatory cytokines/chemokines. TLR3 acts via MYD88/TRAF6 resulting in NFkB activation and cytokine secretion followed by inflammatory response. TLR3 is expressed by epithelial cells, a broad range of antigen-processing cells, tissue dendritic cells, monocytes, mast cells, NK cells etc. TLR3 signaling results in a local cytokine burst which is microenvironment- and cell-specific in nature and leads to local inflammatory response adaptively selected to provide a greatest chance of infection control for affected tissues. TLR3 activation also leads to local production of acute response molecules such as type I IFN, TNF-alpha, IL-12, MCP-1 etc. Defects in TLR3 have been linked to herpes simplex encephalitis type 2 (HSE2) and TLR3 agonists are emerging as promising adjuvants in dendritic cells - based immunotherapy for the treatment of viral and neoplastic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NB100-56722
Species: Hu, Ca
Applications: WB, B/N, Flow, Func, ICC/IF, IHC-P, IP, Flow-CS
NBP2-24729
Species: Hu, Mu, Rt, Ca, Eq, Pm
Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, CyTOF-ready, Flow-IC
NBP2-24906
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC, IF
NB120-13810
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, IB, ICC/IF, IP
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP2-27109
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western, IHC, IHC-P
NBP2-24787
Species: Hu, Mu, Ca
Applications: WB, Flow, IHC, IHC-Fr, IHC-P, Flow-CS, Flow-IC
NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
NBP1-47812
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24917
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC-P, CyTOF-ready, Flow-IC
NBP2-42073
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB110-1244
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-56563
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NB110-93591
Species: Hu, Po, Fe, Pm, Mu, Rt, Bv, Eq
Applications: WB

Publications for TLR3 Antibody (NB110-93591) (0)

There are no publications for TLR3 Antibody (NB110-93591).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for TLR3 Antibody (NB110-93591) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-93591:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TLR3 NB110-93591
Enlarge
reviewed by:
Anonymous
WB Human 12/06/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman macrophages
SpeciesHuman
LotA1

Blocking

Blocking DetailsPBS with 5% non-fat milk

Primary Anitbody

Dilution Ratio1:1000 in PBST(0.05%) with 2% non-fat milk; Overnight, 4oC

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP
Secondary Manufacturer Cat##7074S
Secondary Concentration1:1000

Details

Detection NotesExposure time: 1 min; FujiFilm LAS-1000

Product General Protocols

View specific protocols for TLR3 Antibody (NB110-93591): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR3 Antibody (NB110-93591) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-93591V

Additional TLR3 Products

Bioinformatics Tool for TLR3 Antibody (NB110-93591)

Discover related pathways, diseases and genes to TLR3 Antibody (NB110-93591). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TLR3 Antibody (NB110-93591)

Discover more about diseases related to TLR3 Antibody (NB110-93591).
 

Pathways for TLR3 Antibody (NB110-93591)

View related products by pathway.

PTMs for TLR3 Antibody (NB110-93591)

Learn more about PTMs related to TLR3 Antibody (NB110-93591).
 

Research Areas for TLR3 Antibody (NB110-93591)

Find related products by research area.

Blogs on TLR3.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
12/06/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TLR3
Entrez
Uniprot