TLE4 Antibody

Images

 
Western Blot: TLE4 Antibody [NBP2-15140] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TLE4 Antibody [NBP2-15140] - Paraformaldehyde-fixed HeLa, using TLE4 antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: TLE4 Antibody [NBP2-15140] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.95 mg/ml

Order Details

TLE4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.
Predicted Species
Mouse (100%), Rat (100%), Xenopus (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLE4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.95 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLE4 Antibody

  • BCE1
  • BCE-1
  • E(spI)
  • ESG4
  • homolog of Drosophila E(sp1)
  • KIAA1261
  • Protein BCE-1
  • transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
  • transducin-like enhancer protein 4

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol TLE4
Uniprot