TLE3 Antibody - BSA Free

Western Blot: TLE3 Antibody [NBP2-30646] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH

Immunocytochemistry/Immunofluorescence: TLE3 Antibody [NBP2-30646] - Staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP2-30646] - Staining of human uterine cervix shows moderate to strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP2-30646] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP2-30646] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP2-30646] - Staining of human tonsil shows weak to moderate nuclear positivity in lymphocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TLE3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TLE3 Antibody - BSA Free (NBP2-30646) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: GSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHR
• Predicted Species: Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLE3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TLE3 Protein (NBP2-30646PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TLE3 Antibody - BSA Free

• ESG3
• ESG3Enhancer of split groucho-like protein 3
• ESGenhancer of split groucho 3
• Grg3
• HsT18976
• KIAA1547FLJ39460
• TLE3
• transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• transducin-like enhancer of split 3, homolog of Drosophila E(sp1)
• transducin-like enhancer protein 3

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLE3
• Uniprot:
  • Q04726()