TLE1 Antibody (OTI1H2)

Western Blot: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE1.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human pancreas tissue.

Flow Cytometry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by TLE1 antibody.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human lymphoma tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human prostate tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human Ovary tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Carcinoma of Human pancreas tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Adenocarcinoma of Human breast tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human breast tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Carcinoma of Human prostate tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Carcinoma of Human bladder tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Carcinoma of Human kidney tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human tonsil tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Carcinoma of Human lung tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Adenocarcinoma of Human colon tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Carcinoma of Human liver tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human Kidney tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Human colon tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunofluorescence: TLE1 Antibody (OTI1H2) [NBP2-46134] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TLE1.

TLE1 Antibody (OTI1H2)[NBP2-46134]Simple Western� analysis of endogenous protein Neurofilament (NEFM) from HELA lysates (0.1mg/mL) using NEFM Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TLE1 Antibody (OTI1H2) Summary

• Description: Novus Biologicals Mouse TLE1 Antibody (OTI1H2) (NBP2-46134) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 (NP_005068) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TLE1 Antibody (OTI1H2)

• E(Sp1) homolog
• enhancer of split groucho 1
• ESG
• ESG1Enhancer of split groucho-like protein 1
• GRG1
• TLE1
• transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
• transducin-like enhancer of split 1, homolog of Drosophila E(sp1)
• transducin-like enhancer protein 1

Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLE1