TLE6 Antibody (2E4)

Western Blot: TLE6 Antibody (2E4) [H00079816-M01] - Analysis of TLE6 expression in transfected 293T cell line by TLE6 monoclonal antibody (M01), clone 2E4.Lane 1: TLE6 transfected lysate (Predicted MW: 49.8 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TLE6 Antibody (2E4) Summary

• Immunogen: TLE6 (AAH20206, 1 a.a. ~ 449 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATRSSDWLRRPLGEDNQPETQLFWDKEPWFWHDTLTEQLWRIFAGVHDEKAKPRDRQQAPGLGQESKAPGSCDPGTDPCPEDASTPRPPEASSSPPEGSQDRNTSWGVVQEPPGRASRFLQSISWDPEDFEDAWKRPDALPGQSKRLAVPCKLEKMRILAHGELVLATAISSFTRHVFTCGRRGIKVWSLTGQVAEDRFPESHLPIQTPGAFLRTCLLSSNSRSLLTGGYNLASVSVWDLAAPSLHVKEQLPCAGLNCQALDANLDANLAFASFTSGVVRIWDLRDQSVVRDLKGYPDGVKSIVVKGYNIWTGGPDACLRCWDQRTIMKPLEYQFKSQIMSLSHSPQEDWVLLGMANGQQWLQSTSGSQRHMVGQKDSVILSVKFSPFGQWWASVGMDDFLGVYSMPAGTKVFEVPEMSPVTCCDVSSNNRLVVTGSGEHASVYQITY
• Specificity: TLE6 - transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (2E4)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLE6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry reported in scientific literature (PMID 24356439)

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TLE6 Antibody (2E4)

• GRG6FLJ14009
• MGC14966
• transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila)
• transducin-like enhancer protein 6

Background

As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLE6 Antibody (H00079816-M01)(1)

Publication using H00079816-M01

• Verginelli F, Perin A, Dali R et al. Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth. Nat Commun. 2013-01-01 [PMID: 24356439] (ICC/IF, IHC)

Bioinformatics

• Gene Symbol: TLE6
• Entrez:
  • 79816(Human)
• Uniprot:
  • AAH20206(Human)