TKTL1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TKTL1 Antibody [NBP1-31674] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TKTL1 protein stained by TKTL1 antibody [N1C1] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.

Immunohistochemistry-Paraffin: TKTL1 Antibody [NBP1-31674] - SAS xenograft , using Transketolase-like protein 1 antibody at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: TKTL1 Antibody [NBP1-31674] -Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TKTL1 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TKTL1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TKTL1. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Rhesus Macaque (96%), Chimpanzee (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TKTL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TKTL1 Antibody - BSA Free

• TK 2
• TKR EC 2.2.1.1
• TKR
• TKT2Transketolase 2
• transketolase-2
• transketolase-like 1
• transketolase-like protein 1
• Transketolase-related protein

Background

Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. TKTL1 is a transketolase-related protein. TKTL1 is upregulated in many cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TKTL1 Antibody (NBP1-31674)(2)

Publications using NBP1-31674

• Li Y, Xu C, Wang B et al. Proteomic characterization of gastric cancer response to chemotherapy and targeted therapy reveals new therapeutic strategies Nature communications 2022-09-29 [PMID: 36175412] (WB, Human)
• Li Y, Yao CF, Xu FJ, et al. APC/CCDH1 synchronizes ribose-5-phosphate levels and DNA synthesis to cell cycle progression Nat Commun 2019-06-07 [PMID: 31175280] (WB, Human)

FAQs for TKTL1 Antibody (NBP1-31674). (Showing 1 - 1 of 1 FAQs).

1. Can NBP1-31674 be used for flow cytometry or not?
   • NBP1-31674 has not been tested for Flow Cytometry so there is no guarantee it works in FC, however if you would like to test this antibody in FC, I can suggest our Innovator's Reward program as this program gives an opportunity to the researchers to test an antibody for an untested application/species. Please refer to the link for more information about this program and feel free to contact us if you have any further questions at innovators@novusbio.com.

Bioinformatics

• Gene Symbol: TKTL1
• Entrez:
  • 8277(Human)
• Uniprot:
  • P51854(Human)