PANK2 Antibody (OTI3H9)

Western Blot: PANK2 Antibody (3H9) [NBP2-02677] Analysis of extracts (35ug) from 9 different cell lines by using anti-PANK2 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: PANK2 Antibody (3H9) [NBP2-02677] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PANK2.

Immunohistochemistry-Paraffin: PANK2 Antibody (3H9) [NBP2-02677] - Staining of paraffin-embedded Human prostate tissue using anti-PANK2 mouse monoclonal antibody.

Flow Cytometry: PANK2 Antibody (3H9) [NBP2-02677] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PANK2 antibody, and then analyzed by flow cytometry.

Western Blot: PANK2 Antibody (3H9) [NBP2-02677] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PANK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PANK2.

Immunohistochemistry-Paraffin: PANK2 Antibody (3H9) [NBP2-02677] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PANK2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PANK2 Antibody (3H9) [NBP2-02677] - Staining of paraffin-embedded Human liver tissue using anti-PANK2 mouse monoclonal antibody.

Flow Cytometry: PANK2 Antibody (3H9) [NBP2-02677] - Analysis of Jurkat cells, using anti-PANK2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PANK2 Antibody (OTI3H9) Summary

• Immunogen: Full length human recombinant protein of human PANK2(NP_705902) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PANK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
• Theoretical MW: 59.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PANK2 Antibody (OTI3H9)

• C20orf48
• EC 2.7.1.33
• FLJ11729
• FLJ17232
• Hallervorden-Spatz syndrome
• HARPFLJ11729
• HPANK2
• HSS
• MGC15053
• NBIA1
• PANK2
• pantothenate kinase 2
• pantothenate kinase 2, mitochondrial
• Pantothenic acid kinase 2
• PKAN
• PKANneurodegeneration with brain iron accumulation 1 (Hallervorden-Spatz syndrome)

Background

This gene encodes a protein belonging to the pantothenate kinase family and is the only member of that family to be expressed in mitochondria. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by acyl CoA species. Mutations in this gene are associated with HARP syndrome and pantothenate kinase-associated neurodegeneration (PKAN), formerly Hallervorden-Spatz syndrome. Alternative splicing, involving the use of alternate first exons, results in multiple transcripts encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PANK2
• Entrez:
  • 80025(Human)