DDR2 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with Calyculin A for 10 minutes, loaded at 0.2 mg/mL. A specific band was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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DDR2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human DDR2
Gln24-Arg399
Accession # Q16832
Specificity
Detects human DDR2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human DDR1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDR2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF2538 in the following applications:

Publications
Read Publications using
AF2538 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DDR2 Antibody [Unconjugated]

  • CD167 antigen-like family member B
  • CD167b antigen
  • DDR2
  • Discoidin domain receptor 2
  • discoidin domain receptor family, member 2
  • discoidin domain receptor tyrosine kinase 2
  • discoidin domain-containing receptor 2
  • EC 2.7.10
  • EC 2.7.10.1
  • hydroxyaryl-protein kinase
  • migration-inducing gene 16 protein
  • neurotrophic tyrosine kinase receptor related 3
  • Neurotrophic tyrosine kinase, receptor-related 3
  • NTRKR3cell migration-inducing protein 20
  • Receptor protein-tyrosine kinase TKT
  • TKT
  • TKTMIG20a
  • Trk3
  • TYRO10
  • Tyro-10
  • Tyrosine-protein kinase TYRO10
  • tyrosylprotein kinase

Background

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).

  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Karn, T. et al. (1993) Oncogene 8:3433.
  3. Vogel, W. et al. (1997) Mol. Cell 1:13.
  4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
  6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
  7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
  8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
  9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
  10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
  11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
  12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDR2 Antibody (AF2538)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF2538 Applications Species
T Han, P Mignatti, SB Abramson, M Attur Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration PLoS ONE, 2020;15(4):e0231501. 2020 [PMID: 32330138] (Western Blot, Human) Western Blot Human
AA Jalan, D Sammon, JD Hartgerink, P Brear, K Stott, SW Hamaia, EJ Hunter, DR Walker, B Leitinger, RW Farndale Chain alignment of collagen I deciphered using computationally designed heterotrimers Nat. Chem. Biol., 2020;0(0):. 2020 [PMID: 31907373] (Western Blot, Human) Western Blot Human
I Majkowska, Y Shitomi, N Ito, NS Gray, Y Itoh Discoidin Domain Receptor 2 Mediates Collagen-Induced Activation of Membrane-Type 1 Matrix Metalloproteinase in Human Fibroblasts J. Biol. Chem, 2017;0(0):. 2017 [PMID: 28270508] (Western Blot, Human) Western Blot Human
Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, Paul A, Esposito E, Gridley S, Leitinger B, Naegle K, Huang P Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J, 2013;454(3):501-13. 2013 [PMID: 23822953] (Western Blot, Human) Western Blot Human
DDR2 plays a role in fibroblast migration independent of adhesion ligand and collagen activated DDR2 tyrosine kinase. Biochem Biophys Res Commun, 2012;429(1):39-44. 2012 [PMID: 23131558] (Immunoprecipitation, Human) Immunoprecipitation Human
Kawai I, Hisaki T, Sugiura K, Naito K, Kano K Discoidin domain receptor 2 (DDR2) regulates proliferation of endochondral cells in mice. Biochem Biophys Res Commun, 2012;427(3):611-7. 2012 [PMID: 23022180] (Western Blot, Mouse) Western Blot Mouse
Xu H, Raynal N, Stathopoulos S, Myllyharju J, Farndale RW, Leitinger B Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1. Matrix Biol., 2011;30(1):16-26. 2011 [PMID: 21044884] (Western Blot, Human) Western Blot Human

Reviews for DDR2 Antibody (AF2538) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using AF2538:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21335558
SpeciesHuman
  5
reviewed by:
Anonymous -
IF Human 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23131558
SpeciesHuman
  4
reviewed by:
Anonymous -
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21044884
SpeciesHuman

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous -
01/07/2015
Application: WB
Species: Human

 
Anonymous -
01/07/2015
Application: IF
Species: Human

 
Anonymous -
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol DDR2
Uniprot