DDR2 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with Calyculin A for 10 minutes, loaded at 0.2 mg/mL. A specific band was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

DDR2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human DDR2
Gln24-Arg399
Accession # Q16832
Specificity
Detects human DDR2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human DDR1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control
DDR2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF2538 in the following applications:

Publications
Read Publications using
AF2538 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DDR2 Antibody [Unconjugated]

  • CD167 antigen-like family member B
  • CD167b antigen
  • DDR2
  • Discoidin domain receptor 2
  • discoidin domain receptor family, member 2
  • discoidin domain receptor tyrosine kinase 2
  • discoidin domain-containing receptor 2
  • EC 2.7.10
  • EC 2.7.10.1
  • hydroxyaryl-protein kinase
  • migration-inducing gene 16 protein
  • neurotrophic tyrosine kinase receptor related 3
  • Neurotrophic tyrosine kinase, receptor-related 3
  • NTRKR3cell migration-inducing protein 20
  • Receptor protein-tyrosine kinase TKT
  • TKT
  • TKTMIG20a
  • Trk3
  • TYRO10
  • Tyro-10
  • Tyrosine-protein kinase TYRO10
  • tyrosylprotein kinase

Background

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).

  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Karn, T. et al. (1993) Oncogene 8:3433.
  3. Vogel, W. et al. (1997) Mol. Cell 1:13.
  4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
  6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
  7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
  8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
  9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
  10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
  11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
  12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2396
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-67691
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MMP200
Species: Hu, Mu, Rt, Po, Ca
AF942
Species: Mu
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-31674
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00006464-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA
NBP2-50599
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC

Publications for DDR2 Antibody (AF2538)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF2538 Applications Species
T Han, P Mignatti, SB Abramson, M Attur Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration PLoS ONE, 2020;15(4):e0231501. 2020 [PMID: 32330138] (Western Blot, Human) Western Blot Human
AA Jalan, D Sammon, JD Hartgerink, P Brear, K Stott, SW Hamaia, EJ Hunter, DR Walker, B Leitinger, RW Farndale Chain alignment of collagen I deciphered using computationally designed heterotrimers Nat. Chem. Biol., 2020;0(0):. 2020 [PMID: 31907373] (Western Blot, Human) Western Blot Human
I Majkowska, Y Shitomi, N Ito, NS Gray, Y Itoh Discoidin Domain Receptor 2 Mediates Collagen-Induced Activation of Membrane-Type 1 Matrix Metalloproteinase in Human Fibroblasts J. Biol. Chem, 2017;0(0):. 2017 [PMID: 28270508] (Western Blot, Human) Western Blot Human
Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, Paul A, Esposito E, Gridley S, Leitinger B, Naegle K, Huang P Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J, 2013;454(3):501-13. 2013 [PMID: 23822953] (Western Blot, Human) Western Blot Human
DDR2 plays a role in fibroblast migration independent of adhesion ligand and collagen activated DDR2 tyrosine kinase. Biochem Biophys Res Commun, 2012;429(1):39-44. 2012 [PMID: 23131558] (Immunoprecipitation, Human) Immunoprecipitation Human
Kawai I, Hisaki T, Sugiura K, Naito K, Kano K Discoidin domain receptor 2 (DDR2) regulates proliferation of endochondral cells in mice. Biochem Biophys Res Commun, 2012;427(3):611-7. 2012 [PMID: 23022180] (Western Blot, Mouse) Western Blot Mouse
Xu H, Raynal N, Stathopoulos S, Myllyharju J, Farndale RW, Leitinger B Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1. Matrix Biol., 2011;30(1):16-26. 2011 [PMID: 21044884] (Western Blot, Human) Western Blot Human

Reviews for DDR2 Antibody (AF2538) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using AF2538:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21335558
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21335558

Details

Detection NotesSee PMID 21335558

Comments

Comments1:100 dilutionPublished: 1:100 dilution
  5
reviewed by:
Anonymous
IF Human 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23131558
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23131558

Details

Detection NotesSee PMID 23131558

Comments

CommentsPublished: See PMID 23131558
  4
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21044884
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21044884

Details

Detection NotesSee PMID 21044884

Comments

CommentsPublished: See PMID 21044884

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DDR2 Antibody (AF2538) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2538

Additional DDR2 Products

Bioinformatics Tool for DDR2 Antibody (AF2538)

Discover related pathways, diseases and genes to DDR2 Antibody (AF2538). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DDR2 Antibody (AF2538)

Discover more about diseases related to DDR2 Antibody (AF2538).
 

Pathways for DDR2 Antibody (AF2538)

View related products by pathway.

PTMs for DDR2 Antibody (AF2538)

Learn more about PTMs related to DDR2 Antibody (AF2538).

Blogs on DDR2

There are no specific blogs for DDR2, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

DDR2 [Biotin]
DYC6170-2

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: WB
Species: Human

 
Anonymous
01/07/2015
Application: IF
Species: Human

 
Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol DDR2
Uniprot