Recombinant Human TIM-3 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Human TIM-3 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin‑9 (Catalog # 2045-GA) at 500 ng/mL can bind Recombinant Human TIM-3 Fc Chimera with an apparent Kd <30 nM.
Source
Mouse myeloma cell line, NS0-derived human TIM-3 protein
Human TIM-3
(Ser22-Arg200)
Accession # Q8TDQ0
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
HAVCR2
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
46.4 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
66 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
using
2365-TM in the following application:

Publications
Read Publications using
2365-TM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TIM-3 Fc Chimera Protein, CF

  • CD366
  • FLJ14428
  • HAVCR2
  • HAVcr-2
  • hepatitis A virus cellular receptor 2
  • kidney injury molecule-3
  • KIM-3
  • SPTCL
  • T cell immunoglobulin mucin 3
  • T cell immunoglobulin mucin-3
  • T-cell immunoglobulin and mucin domain-containing protein 3
  • TIM 3
  • TIM3 T-cell membrane protein 3
  • TIM3
  • TIM-3
  • TIMD3
  • TIMD-3
  • TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).
  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. Monney, L. et al. (2002) Nature 415:536.
  4. Nakayama, M. et al. (2009) Blood 113:3821.
  5. Anderson, A.C. et al. (2007) Science 318:1141.
  6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  10. Gleason, M.K. et al. (2012) Blood 119:3064.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

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2365-TM
Species: Hu
Applications: Binding Activity

Publications for TIM-3 (2365-TM)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Bacteria.

We have publications tested in 4 applications: Binding Assay, Bioassay, Enzyme Assay, Enzyme Assay Substrate.


Filter By Application
Binding Assay
(1)
Bioassay
(6)
Enzyme Assay
(1)
Enzyme Assay Substrate
(1)
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Human
(7)
Mouse
(1)
Bacteria
(1)
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Showing Publications 1 - 9 of 9.
Publications using 2365-TM Applications Species
S de Kivit, AI Kostadinov, J Kerperien, ME Morgan, VA Muruzabal, GA Hofman, LMJ Knippels, AD Kraneveld, J Garssen, LEM Willemsen Dietary, nondigestible oligosaccharides and Bifidobacterium breve M-16V suppress allergic inflammation in intestine via targeting dendritic cell maturation J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28495789] (Bioassay, Human) Bioassay Human
Wang S, Cao C, Piao H, Li Y, Tao Y, Zhang X, Zhang D, Sun C, Zhu R, Wang Y, Yuan M, Li D, Du M Tim-3 protects decidual stromal cells from toll-like receptor-mediated apoptosis and inflammatory reactions and promotes Th2 bias at the maternal-fetal interface., 2015;0(0):. 2015 [PMID: 25757669] (Bioassay, Human) Bioassay Human
Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes. PLoS ONE, 2014;9(9):e107920. 2014 [PMID: 25251283] (Enzyme Assay Substrate, Bacteria) Enzyme Assay Substrate Bacteria
Kojima R, Ohno T, Iikura M, Niki T, Hirashima M, Iwaya K, Tsuda H, Nonoyama S, Matsuda A, Saito H, Matsumoto K, Nakae S Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line. PLoS ONE, 2014;9(1):e86106. 2014 [PMID: 24465902] (Bioassay, Human) Bioassay Human
Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter TIM-3 does not act as a receptor for galectin-9. PLoS Pathog, 2013;9(3):e1003253. 2013 [PMID: 23555261] (Bioassay, Mouse) Bioassay Mouse
Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection. PLoS ONE, 2012;7(10):e47648. 2012 [PMID: 23112829] (Bioassay, Human) Bioassay Human
Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z Blockade of Tim-3 signaling restores the virus-specific CD8+ T-cell response in patients with chronic hepatitis B. Eur. J. Immunol., 2012;42(5):1180-91. 2012 [PMID: 22539292] (Bioassay, Human) Bioassay Human
Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin. PLoS ONE, 2011;6(3):e17787. 2011 [PMID: 21412435] (Binding Assay, Human) Binding Assay Human
Vega-Carrascal I, Reeves EP, Niki T, Arikawa T, McNally P, O'Neill SJ, Hirashima M, McElvaney NG Dysregulation of TIM-3-Galectin-9 Pathway in the Cystic Fibrosis Airways. J. Immunol., 2011;0(0):. 2011 [PMID: 21263071] (Enzyme Assay, Human) Enzyme Assay Human

Review for TIM-3 (2365-TM) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 2365-TM:
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 TIM-3 2365-TM
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5
reviewed by:
Anonymous
08/10/2018
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Summary

LotHKG1217021

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TIM-3, a critical immune checkpoint in HIV research
CD4+ T-helper cells (Th) are the white blood lymphocytes expressing surface glycoprotein antigen CD4. These T-helper cells play an important role in the adaptive immune system by releasing T cell cytokines that help other immune cells to suppress o...  Read full blog post.

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Bioinformatics

Gene Symbol HAVCR2
Uniprot