TIM-3 Antibody (2321C) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: Flow
• Clone: 2321C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

TIM-3 Antibody (2321C) [Alexa Fluor® 700] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIM-3
  Met1-Arg200
  Accession # Q8TDQ0
• Specificity: Detects human TIM-3 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody (2321C) [Alexa Fluor® 700]

• CD366
• FLJ14428
• HAVCR2
• HAVcr-2
• hepatitis A virus cellular receptor 2
• kidney injury molecule-3
• KIM-3
• SPTCL
• T cell immunoglobulin mucin 3
• T cell immunoglobulin mucin-3
• T-cell immunoglobulin and mucin domain-containing protein 3
• TIM 3
• TIM3 T-cell membrane protein 3
• TIM3
• TIM-3
• TIMD3
• TIMD-3
• TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4⁺ Th1 and CD8⁺ Tc1) but not on helper T cells (CD4⁺ Th2 and CD8⁺ Tc2) (4, 5). NK cells appear to transcribe the highest amounts of Tim-3 among lymphocytes, and when Tim-3 was cross-linked with antibodies it suppressed NK cell-mediated cytotoxicity (6). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11), and regulatory T cell development (5). TIM-3 inhibits the antitumor efficacy of DNA vaccines and chemotherapy by binding to the damage-associated molecular pattern molecule, HMGB1 (13).

1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B. 360:1681.
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
4. Monney, L. et al. (2002) Nature 415:536.
5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
6. Ndhlovu, L. et al. (2012) Blood 119:3734.
7. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
8. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
9. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
10. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
11. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
12. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
13. Chiba, S. et al. (2012) Nat. Immunol. 13:832.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8TDQ0()