TIF1 gamma Antibody - BSA Free

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP2-98000] - Immunochemical staining of human TIF1 gamma in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP2-98000] - Immunochemical staining of human TIF1 gamma in human adrenal gland with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

Immunocytochemistry/Immunofluorescence: TIF1 gamma Antibody [NBP2-98000] - Immunofluorescence staining of TIF1 gamma in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98000 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TIF1 gamma Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human TIF1 gamma fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM33
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for TIF1 gamma Antibody - BSA Free

• E3 ubiquitin-protein ligase TRIM33
• EC 6.3.2
• EC 6.3.2.-
• Ectodermin homolog
• KIAA1113
• Protein Rfg7
• PTC7FLJ32925
• RET-fused gene 7 protein
• RFG7FLJ11429
• TF1G
• TIF1GAMMA
• TIF1-gamma
• TIF1GECTO
• TIFGAMMA
• Transcription intermediary factor 1-gamma
• transcriptional intermediary factor 1 gamma
• tripartite motif containing 33
• tripartite motif-containing 33
• Tripartite motif-containing protein 33

Background

TIF1 gamma is encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM33