Nogo Receptor/NgR Antibody

NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.

NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.

NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Nogo Receptor/NgR Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse NgR
  Cys27-Ser447
  Accession # Q99PI8
• Specificity: Detects mouse Nogo Receptor in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant human (rh) NgR is observed and less than 1% cross-reactivity with rhNgRH1 and rhNgRH2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RTN4R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nogo Receptor/NgR Antibody

• NgR
• NgR1
• Nogo R
• Nogo receptor
• Nogo-66 receptor
• NogoR
• NOGORUNQ330/PRO526
• reticulon 4 receptor
• reticulon-4 receptor
• RTN4R

Background

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid (aa) residue precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75^NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75^NTR. The activated p75^NTR  then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

1. Fournier, A.E. et al. (2001) Nature 409:341.
2. GrandPre, T. et al. (2002) Nature 417:547.
3. Wang, K.C. et al. (2002) Nature 420:74.
4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.
5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nogo Receptor/NgR Antibody (AF1440)(18)

Publications using AF1440

• S Fricke, K Metzdorf, M Ohm, S Haak, M Heine, M Korte, M Zagrebelsk Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling Cell Rep, 2019-10-15;29(3):671-684.e6. 2019-10-15 [PMID: 31618635] (Neutralization, Mouse)
• Rhalena A. Thomas, Julien Gibon, Carol X. Q. Chen, Sabrina Chierzi, Vincent G. Soubannier, Stephanie Baulac et al. The Nogo Receptor Ligand LGI1 Regulates Synapse Number and Synaptic Activity in Hippocampal and Cortical Neurons eNeuro 2018-08-27 [PMID: 30225353]
• Stephany CE, Ma X, Dorton HM et al. Distinct Circuits for Recovery of Eye Dominance and Acuity in Murine Amblyopia Curr Biol 6/12/2018 [PMID: 29887305]
• AM Solomon, T Westbrook, GD Field, AW McGee Nogo receptor 1 is expressed by nearly all retinal ganglion cellsPV expression in Figure 3 PLoS ONE, 2018-05-16;13(5):e0196565. 2018-05-16 [PMID: 29768445] (IHC, Mouse)
• Yuichi Sekine, Chad S. Siegel, Tomoko Sekine-Konno, William B. J. Cafferty, Stephen M. Strittmatter The nociceptin receptor inhibits axonal regeneration and recovery from spinal cord injury Science Signaling 2018-04-05 [PMID: 29615517]
• Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor J. Neurosci., 2018-02-09;0(0):. 2018-02-09 [PMID: 29440387] (ICC, Mouse)
• Yanjun Zhao, Sivaprakash Sivaji, Michael C. Chiang, Haadi Ali, Monica Zukowski, Sareen Ali et al. Amyloid Beta Peptides Block New Synapse Assembly by Nogo Receptor-Mediated Inhibition of T-Type Calcium Channels Neuron 2017-10-11 [PMID: 29024660]
• T Hirokawa, Y Zou, Y Kurihara, Z Jiang, Y Sakakibara, H Ito, K Funakoshi, N Kawahara, Y Goshima, SM Strittmatt, K Takei Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS Sci Rep, 2017-09-21;7(1):12119. 2017-09-21 [PMID: 28935984] (Western Blot, Mouse)
• H Takase, Y Kurihara, TA Yokoyama, N Kawahara, K Takei LOTUS overexpression accelerates neuronal plasticity after focal brain ischemia in mice PLoS ONE, 2017-09-07;12(9):e0184258. 2017-09-07 [PMID: 28880879] (Western Blot, Mouse)
• Sarah M. Bhagat, Santino S. Butler, Jane R. Taylor, Bruce S. McEwen, Stephen M. Strittmatter Erasure of fear memories is prevented by Nogo Receptor 1 in adulthood Molecular Psychiatry 2016-09-01 [PMID: 26619810]
• Vajda F, Jordi N, Dalkara D, Joly S, Christ F, Tews B, Schwab M, Pernet V Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve. Cell Death Differ, 2014-09-26;22(2):323-35. 2014-09-26 [PMID: 25257170] (IHC, Mouse)
• Stephany C, Chan L, Parivash S, Dorton H, Piechowicz M, Qiu S, McGee A Plasticity of binocularity and visual acuity are differentially limited by nogo receptor. J Neurosci, 2014-08-27;34(35):11631-40. 2014-08-27 [PMID: 25164659] (IHC, Mouse)
• Xingxing Wang, Kazim Yigitkanli, Chang-Yeon Kim, Tomoko Sekine-Konno, Dana Wirak, Eric Frieden et al. Human NgR-Fc Decoy Protein via Lumbar Intrathecal Bolus Administration Enhances Recovery from Rat Spinal Cord Contusion Journal of Neurotrauma 2014-12-15 [PMID: 24964223]
• Thomas R, Favell K, Morante-Redolat J, Pool M, Kent C, Wright M, Daignault K, Ferraro GB, Montcalm S, Durocher Y, Fournier A, Perez-Tur J, Barker PA LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition. J. Neurosci., 2010-05-12;30(19):6607-12. 2010-05-12 [PMID: 20463223] (Western Blot, Human)
• Peng X, Zhou Z, Hu J, Fink DJ, Mata M Soluble Nogo receptor down-regulates expression of neuronal Nogo-A to enhance axonal regeneration. J. Biol. Chem., 2009-11-09;285(4):2783-95. 2009-11-09 [PMID: 19901030] (Western Blot, Rat)
• Wang J, Wang L, Zhao H, Chan SO Localization of an axon growth inhibitory molecule Nogo and its receptor in the spinal cord of mouse embryos. Brain Res., 2009-10-13;1306(0):8-17. 2009-10-13 [PMID: 19833111] (IHC, Mouse)
• Lee JK, Chan AF, Luu SM, Zhu Y, Ho C, Tessier-Lavigne M, Zheng B Reassessment of corticospinal tract regeneration in Nogo-deficient mice. J. Neurosci., 2009-07-08;29(27):8649-54. 2009-07-08 [PMID: 19587271] (Western Blot, Mouse)
• Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength. J. Neurosci., 2008-03-12;28(11):2753-65. 2008-03-12 [PMID: 18337405] (Western Blot, Rat)

Review for Nogo Receptor/NgR Antibody (AF1440) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 12/19/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse neurons DIV12
• Species: Mouse

Bioinformatics

• Gene Symbol: RTN4R
• Uniprot:
  • Q99PI8()