Nogo Receptor/NgR Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Nogo Receptor/NgR Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NgR
Cys27-Ser447
Accession # Q99PI8
Specificity
Detects mouse Nogo Receptor in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant human (rh) NgR is observed and less than 1% cross-reactivity with rhNgRH1 and rhNgRH2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RTN4R
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
AF1440 in the following application:

Publications
Read Publications using
AF1440 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nogo Receptor/NgR Antibody

  • NgR
  • NgR1
  • Nogo R
  • Nogo receptor
  • Nogo-66 receptor
  • NogoR
  • NOGORUNQ330/PRO526
  • reticulon 4 receptor
  • reticulon-4 receptor
  • RTN4R

Background

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid (aa) residue precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR  then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

  1. Fournier, A.E. et al. (2001) Nature 409:341.
  2. GrandPre, T. et al. (2002) Nature 417:547.
  3. Wang, K.C. et al. (2002) Nature 420:74.
  4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.
  5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
  6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nogo Receptor/NgR Antibody (AF1440)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, IHC OCT-embedded, IHC- Not specified, WB.


Filter By Application
ICC
(1)
IHC
(2)
IHC OCT-embedded
(1)
IHC- Not specified
(1)
WB
(6)
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Human
(1)
Mouse
(8)
Rat
(2)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF1440 Applications Species
AM Solomon, T Westbrook, GD Field, AW McGee Nogo receptor 1 is expressed by nearly all retinal ganglion cellsPV expression in Figure 3 PLoS ONE, 2018;13(5):e0196565. 2018 [PMID: 29768445] (IHC,, Mouse) IHC Mouse
Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor J. Neurosci., 2018;0(0):. 2018 [PMID: 29440387] (ICC,, Mouse) ICC Mouse
T Hirokawa, Y Zou, Y Kurihara, Z Jiang, Y Sakakibara, H Ito, K Funakoshi, N Kawahara, Y Goshima, SM Strittmatt, K Takei Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS Sci Rep, 2017;7(1):12119. 2017 [PMID: 28935984] (WB,, Mouse) WB Mouse
H Takase, Y Kurihara, TA Yokoyama, N Kawahara, K Takei LOTUS overexpression accelerates neuronal plasticity after focal brain ischemia in mice PLoS ONE, 2017;12(9):e0184258. 2017 [PMID: 28880879] (WB,, Mouse) WB Mouse
Vajda F, Jordi N, Dalkara D, Joly S, Christ F, Tews B, Schwab M, Pernet V Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve. Cell Death Differ, 2015;22(2):323-35. 2015 [PMID: 25257170] (IHC OCT-embedded,, Mouse) IHC OCT-embedded Mouse
Stephany C, Chan L, Parivash S, Dorton H, Piechowicz M, Qiu S, McGee A Plasticity of binocularity and visual acuity are differentially limited by nogo receptor. J Neurosci, 2014;34(35):11631-40. 2014 [PMID: 25164659] (IHC- Not specified,, Mouse) IHC- Not specified Mouse
Thomas R, Favell K, Morante-Redolat J, Pool M, Kent C, Wright M, Daignault K, Ferraro GB, Montcalm S, Durocher Y, Fournier A, Perez-Tur J, Barker PA LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition. J. Neurosci., 2010;30(19):6607-12. 2010 [PMID: 20463223] (WB,, Human) WB Human
Peng X, Zhou Z, Hu J, Fink DJ, Mata M Soluble Nogo receptor down-regulates expression of neuronal Nogo-A to enhance axonal regeneration. J. Biol. Chem., 2010;285(4):2783-95. 2010 [PMID: 19901030] (WB,, Rat) WB Rat
Wang J, Wang L, Zhao H, Chan SO Localization of an axon growth inhibitory molecule Nogo and its receptor in the spinal cord of mouse embryos. Brain Res., 2010;1306(0):8-17. 2010 [PMID: 19833111] (IHC,, Mouse) IHC Mouse
Lee JK, Chan AF, Luu SM, Zhu Y, Ho C, Tessier-Lavigne M, Zheng B Reassessment of corticospinal tract regeneration in Nogo-deficient mice. J. Neurosci., 2009;29(27):8649-54. 2009 [PMID: 19587271] (WB,, Mouse) WB Mouse
Show All 11 Publications.

Review for Nogo Receptor/NgR Antibody (AF1440) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1440:
Filter by Applications
IF
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 12/19/2014
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Summary

ApplicationImmunofluorescence
Sample TestedMouse neurons DIV12
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1 ug/ml

Details

Detection Notesn/a
Fixation DetailsPFA-fixed

Comments

CommentsThree-star review on pAbmAbs http://pabmabs.com/

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Anonymous
12/19/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol RTN4R
Uniprot