Recombinant Human ENPP-2/Autotaxin Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

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Recombinant Human ENPP-2/Autotaxin Protein, CF Summary

Details of Functionality
Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >8,000 pmol/min/μg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human ENPP-2/Autotaxin protein
Asp49-Ile863, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Enzyme Activity
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
99 kDa and 103 kDa, migrates as a doublet under reducing conditions
Reviewed Applications
Read 1 Review rated 5
5255-EN in the following application:

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5255-EN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 5 mM MgCl2, 0.02% Brij-35 (w/v), pH 8.5
  • Recombinant Human ENPP‑2/Autotaxin (rhENPP-2) (Catalog # 5255-EN)
  • Substrate: Bis(p-Nitrophenyl) Phosphate Sodium Salt (BPNPP) (Sigma, Catalog # N3002), 40 mM stock in deionized water (Note: Heating may be necessary to solubilize Substrate.)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhENPP-2 to 0.1 ng/µL in Assay Buffer.
  2. Dilute Substrate BPNPP to 2 mM in Assay Buffer.
  3. Load into the wells of a microplate 50 µL of 0.1 ng/µL rhENPP-2 and start the reaction by adding 50 µL of 2 mM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL Substrate.
  4. Read at 400 nm (absorbance) in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard p-Nitrophenol (Sigma-Aldrich, Catalog # 241326).

Per Well:
  • rhENPP-2: 0.005 µg
  • Substrate: 1 mM


This product may be covered by one or more of the following US issued patents 5,449,753; 5,731,167; 6,084,069; 6,417,338 and US pending applications.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ENPP-2/Autotaxin Protein, CF

  • ATX
  • ATXFLJ26803
  • ATX-X
  • Autotaxin
  • autotaxin-t
  • EC
  • ectonucleotide pyrophosphatase/phosphodiesterase 2
  • ectonucleotide pyrophosphatase/phosphodiesterase family member 2
  • E-NPP 2
  • ENPP2
  • ENPP-2
  • Extracellular lysophospholipase D
  • Lysophosphatidic Acid
  • LysoPLD
  • NPP2
  • PDNP2
  • phosphodiesterase I/nucleotide pyrophosphatase 2
  • plasma lysophospholipase D


ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain (1). Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine (2). The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site (2). Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2 (3). ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis (4) and to be up-regulated in several types of carcinomas including breast and lung (5).

  1. Cimpean, A. et al. (2004) Biochem. J. 381:71.
  2. Gijsbers, R. et al. (2003) FEBS Letters. 538:60.
  3. Van Meeteren, L.A. et al. (2005) J. Biol. Chem. 280:21155.
  4. Nam, S.W. et al. (2000) Oncogene 19:241.
  5. Jansen, S. et al. (2005) J. Cell Sci. 118:3081.

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Publications for ENPP-2/Autotaxin (5255-EN)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.

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Showing Publications 1 - 5 of 5.
Publications using 5255-EN Applications Species
N Sakai, G Bain, K Furuichi, Y Iwata, M Nakamura, A Hara, S Kitajima, A Sagara, T Miyake, T Toyama, K Sato, S Nakagawa, M Shimizu, S Kaneko, T Wada The involvement of autotaxin in renal interstitial fibrosis through regulation of fibroblast functions and induction of vascular leakage Sci Rep, 2019-05-15;9(1):7414. 2019-05-15 [PMID: 31092842] (Bioassay, Human) Bioassay Human
T Pleli, A Mondorf, N Ferreiros, D Thomas, K Dvorak, RM Biondi, DMZ Heringdorf, S Zeuzem, G Geisslinge, H Zimmermann, O Waidmann, A Piiper Activation of Adenylyl Cyclase Causes Stimulation of Adenosine Receptors Cell. Physiol. Biochem., 2018-03-27;45(6):2516-2528. 2018-03-27 [PMID: 29587249] (Bioassay) Bioassay
S She, M Yang, H Hu, P Hu, Y Yang, H Ren Proteomics Based Identification of Autotaxin As An Anti-Hepatitis B Virus Factor and a Promoter of Hepatoma Cell Invasion and Migration Cell. Physiol. Biochem., 2018-02-01;45(2):744-760. 2018-02-01 [PMID: 29414802] (Bioassay, Human) Bioassay Human
G Zhang, Y Cheng, Q Zhang, X Li, J Zhou, J Wang, L Wei ATX?LPA axis facilitates estrogen?induced endometrial cancer cell proliferation via MAPK/ERK signaling pathway Mol Med Rep, 2018-01-08;0(0):. 2018-01-08 [PMID: 29328374] (Bioassay, Human) Bioassay Human
Ryu J, Han H Autotaxin-LPA axis regulates hMSC migration by adherent junction disruption and cytoskeletal rearrangement via LPAR1/3-dependent PKC/GSK3beta/beta-catenin and PKC/Rho GTPase pathways. Stem Cells, 2015-03-01;33(3):819-32. 2015-03-01 [PMID: 25376707] (Bioassay, Human) Bioassay Human

Review for ENPP-2/Autotaxin (5255-EN) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 5255-EN:
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Enzymatic activity in vitro
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Enzymatic activity in vitro ENPP-2/Autotaxin 5255-EN
reviewed by:
Verified Customer
Enzymatic activity in vitro 08/12/2023


ApplicationEnzymatic activity in vitro

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Application: Enzymatic activity in vitro


Gene Symbol ENPP2