USP9x Antibody

Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells

Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.

Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.

Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.

Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

USP9x Antibody Summary

• Description: Novus Biologicals Rabbit USP9x Antibody (NBP1-48321) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-USP9x Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Genomic peptide made to an internal region of the human USP9x protein (within residues 1150-1300). [Swiss-Prot Q93008]
• Localization: Cytoplasm
• Predicted Species: Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP9X
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:25-1:200
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Application Notes: This USP9x antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~270 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 270 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (95%) and zebrafish (80%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1% BSA, and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for USP9x Antibody

• Deubiquitinating enzyme FAF-X
• DFFRX
• DFFRXubiquitin specific protease 9, X-linked (fat facets-like, Drosophila)
• Drosophila fat facets related, X-linked
• EC 3.1.2.15
• EC 3.4.19.12
• FAF
• FAM
• Fat facets in mammals
• fat facets protein related, X-linked
• Fat facets protein-related, X-linked
• hFAM
• probable ubiquitin carboxyl-terminal hydrolase FAF-X
• ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila)
• ubiquitin specific peptidase 9, X-linked
• ubiquitin thioesterase FAF-X
• Ubiquitin thiolesterase FAF-X
• ubiquitin-specific processing protease FAF-X
• Ubiquitin-specific protease 9, X chromosome
• Ubiquitin-specific-processing protease FAF-X
• USP9
• USP9x
• X chromosome (fat facets-like Drosophila)

Background

USP9x (ubiquitin-specific protease 9, X chromosome) is one of the largest deubiquitinases (DUBs) belonging to the Ub-specific protease family and implicates in processing of ubiquitin precursors as well as of ubiquitinated proteins, thereby playing a key regulatory role at protein turnover level by preventing degradation of proteins through conjugated ubiquitin removal. USP9X substrates include AF-6, beta-catenin, NUAK1, MARK4, ErbB2, EFA6, Smad4, Mcl1, ASK1, and survivin, and USP9X has been involved in several biological processes that include: protein trafficking and tight junction biogenesis in epithelial cells; regulation of the TGF-beta pathway; chromosome alignment and segregation; component of PEX5-mediated peroxisomal protein import pathway. Moreover, USP9X modulates the proteins levels of: alpha-synuclein, a protein involved in the pathogenesis of Parkinson disease; MCL1, an anti-apoptotic protein generally found overexpressed in cancers; some protein kinases involved in stress responses and cellular energy homeostasis; and some ubiquitin-ligases, such as MARCH7 and Itch.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP9x Antibody (NBP1-48321)(6)

Publications using NBP1-48321

• Haiquan Lu, Yajing Lyu, Linh Tran, Jie Lan, Yangyiran Xie, Yongkang Yang, Naveena L Murugan, Yueyang J Wang, Gregg L Semenza HIF-1 recruits NANOG as a coactivator for TERT gene transcription in hypoxic breast cancer stem cells. Cell reports 2022-02-10 [PMID: 34592152]
• Bodiga Vl, Vemuri Pk, Nimmagadda G, Bodiga S Zinc-dependent changes in oxidative and endoplasmic reticulum stress during cardiomyocyte hypoxia/reoxygenation Biol. Chem. 2020-06-01 [PMID: 32549180] (WB)
• Asahina, M, Fujinawa, R Et al. Ngly1 -/- rats develop neurodegenerative phenotypes and pathological abnormalities in their peripheral and central nervous systems. Hum Mol Genet 2020-06-27 [PMID: 32259258] (WB, Mouse)
• Dietachmayr M, Rathakrishnan A, Karpiuk O et Al. Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival Nat Commun 2020-03-09 [PMID: 32152317] (WB, Human)
• Perez-Mancera PA, Rust AG, van der Weyden L et al. The deubiquitinase USP9X suppresses pancreatic ductal adenocarcinoma. Nature 2012-04-01 [PMID: 22699621] (IF/IHC, Human)
• Tian R, Cheng H-YM, Figeys D. Uncovering the proteome response of the master circadian clock to light using an AutoProteome system. Mol Cell Proteomics. 2011-08-22 [PMID: 21859948] (ICC/IF, Mouse)

Review for USP9x Antibody (NBP1-48321) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Andrea Cerase IF Mouse 08/15/2011

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse ES cell line (129)
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: USP9X
• Entrez:
  • 8239(Human)
  • 22284(Mouse)
• Uniprot:
  • Q93008(Human)
  • P70398(Mouse)