USP9x Antibody

Images

 
Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.
Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.
Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

USP9x Antibody Summary

Immunogen
Genomic peptide made to an internal region of the human USP9x protein (within residues 1150-1300). [Swiss-Prot Q93008]
Localization
Cytoplasm
Predicted Species
Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP9X
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:25-1:200
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This USP9x antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~270 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
270 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-48321 in the following applications:

Publications
Read Publications using
NBP1-48321 in the following applications:

Reactivity Notes

Human and mouse. Immunogen sequence has 95% homology to rat and 80% homology to Zebrafish.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for USP9x Antibody

  • Deubiquitinating enzyme FAF-X
  • DFFRX
  • DFFRXubiquitin specific protease 9, X-linked (fat facets-like, Drosophila)
  • Drosophila fat facets related, X-linked
  • EC 3.1.2.15
  • EC 3.4.19.12
  • FAF
  • FAM
  • Fat facets in mammals
  • fat facets protein related, X-linked
  • Fat facets protein-related, X-linked
  • hFAM
  • probable ubiquitin carboxyl-terminal hydrolase FAF-X
  • ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila)
  • ubiquitin specific peptidase 9, X-linked
  • ubiquitin thioesterase FAF-X
  • Ubiquitin thiolesterase FAF-X
  • ubiquitin-specific processing protease FAF-X
  • Ubiquitin-specific protease 9, X chromosome
  • Ubiquitin-specific-processing protease FAF-X
  • USP9
  • USP9x
  • X chromosome (fat facets-like Drosophila)

Background

USP9x (ubiquitin-specific protease 9, X chromosome) is one of the largest deubiquitinases (DUBs) belonging to the Ub-specific protease family and implicates in processing of ubiquitin precursors as well as of ubiquitinated proteins, thereby playing a key regulatory role at protein turnover level by preventing degradation of proteins through conjugated ubiquitin removal. USP9X substrates include AF-6, beta-catenin, NUAK1, MARK4, ErbB2, EFA6, Smad4, Mcl1, ASK1, and survivin, and USP9X has been involved in several biological processes that include: protein trafficking and tight junction biogenesis in epithelial cells; regulation of the TGF-beta pathway; chromosome alignment and segregation; component of PEX5-mediated peroxisomal protein import pathway. Moreover, USP9X modulates the proteins levels of: alpha-synuclein, a protein involved in the pathogenesis of Parkinson disease; MCL1, an anti-apoptotic protein generally found overexpressed in cancers; some protein kinases involved in stress responses and cellular energy homeostasis; and some ubiquitin-ligases, such as MARCH7 and Itch.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP9x Antibody (NBP1-48321)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IHC.


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ICC/IF
(1)
IHC
(1)
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Human
(1)
Mouse
(1)
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Review for USP9x Antibody (NBP1-48321) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-48321:
Filter by Applications
IF
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence USP9x NBP1-48321
Enlarge
reviewed by:
Andrea Cerase
IF Mouse 08/15/2011
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse ES cell line (129)
SpeciesMouse
LotA1
CommentsThis antibody has been tested in a mouse male ES cell line (129-derived) by IF using a 1:100 dilution.The protein localizes to the cytoplasm. USP9x in Red, DNA (DAPI) in blue. It may localize to the RE (RE markers and double IF experiment necessary to confirm it).

Blocking

Blocking DetailsBlocking Buffer: FISH gelatin (1g in 500ml) + 5% goat serum, Blocking Time: 3-5 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: FISH gelatin (1g in 500ml) + 5% goat serum, Dilution Ratio: 1:100, Incubation Time: 2hr, Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa Fluor 568 goat anti mouse IgG- highly cross adsordeb, Secondary Ab Dilution: 1:300
Secondary Manufacturer Cat#A11031

Details

Detection NotesDetection Method: fluorescence at 568, Pos Ctrl: ES cell line, Neg Ctrl: K27me3 on a hetero-chromatinized ch5
Fixation DetailsSample Preparation: PFA fixing + permeabilization

Comments

CommentsThis antibody has been tested in a mouse male ES cell line (129-derived) by IF using a 1:100 dilution.The protein localizes to the cytoplasm. USP9x in Red, DNA (DAPI) in blue. It may localize to the RE (RE markers and double IF experiment necessary to confirm it).

Product General Protocols

View specific protocols for USP9x Antibody (NBP1-48321): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for USP9x Antibody (NBP1-48321) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48321AF405
Alexa Fluor 488 Labeled NBP1-48321AF488
Alexa Fluor 647 Labeled NBP1-48321AF647
Alexa Fluor 700 Labeled NBP1-48321AF700
Biotin Labeled NBP1-48321B
DyLight 488 Labeled NBP1-48321G
DyLight 550 Labeled NBP1-48321R
DyLight 650 Labeled NBP1-48321C
HRP Labeled NBP1-48321H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Andrea Cerase
08/15/2011
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol USP9X
Entrez
Uniprot