Tie-2 Antibody [Unconjugated]

Tie‑2 was detected in immersion fixed paraffin-embedded sections of human intestine tissue using Goat Anti-Mouse/Rat Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF762) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Pericytes express functional Tie2.(a) Microarray-based expression screening of brain pericytes (BP), placenta pericytes (PP), pancreas pericytes (PA), lung pericytes (LP), muscle pericytes (MP) and human umbilical vein endothelial cells (HUVEC [HU]). (b) Semi-quantitative PCR of TEK (Tie2), endothelial marker genes (PECAM1, CDH5) and pericyte marker genes (CSPG4, CD248) in HUVEC and BP; house keeping gene: GAPDH. (c) Representative images showing expression of Tie2 in HUVEC and BP. (d) Histogram of membrane-bound Tie2 expression in HUVEC and BP compared to isotype control measured by flow cytometry. (e,g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells. (f,h) Quantification of the ratio of phosphorylated Tie2 (pTie2) relative to total Tie2 protein in BP (f) and HUVEC (h) normalized to us control (n=3). (i,k) Western blot analysis of AKT phosphorylation (Ser473) in control (shCtr) and Tie2-silenced (shTie2 I/shTie2 II) BP (i) and HUVEC (k) upon stimulation with rhAng1 or rhAng2. (j,l) Quantification of the ratio of pAKT to total AKT protein expression in BP (j) and HUVEC (l) normalized to unstimulated control (n=3). Representative western blot images are cropped versions and original images can be found in Supplementary Fig. 15. Scale bars: 20 μm (c). Data are shown as mean±s.d. Statistics were performed using Mann–Whitney U test (f,h) and one-way ANOVA (j,l). *P<0.05, **P<0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28719590), licensed under a CC-BY license. Not internally tested by R&D Systems.

Endothelial changes after pericyte depletion. a–f Maximum intensity projection of confocal images from control and DTRiPC P6 retinas stained for IB4 (red) in combination with VEGF-A a, VEGFR2 b, VEGFR3 c, Tie2 d, Esm1 e, and Dll4 f (all in white), as indicated. Note local increase of VEGFR2, VEGFR3, and Esm1 (arrowheads in b, c, e) but not Tie2 or VEGF-A at the edge of the vessel plexus. Dll4 expression in DTRiPC sprouts is increased in some regions (arrowheads) but absent in others (arrows). Scale bar, 100 µm. g–j Quantitation of VEGF-A immunosignals area and intensity g, signal intensity for VEGFR2 h and VEGFR3 i and proportion of Esm1+ area with respect to vascular area j in the P6 control and DTRiPC angiogenic front. Error bars, s.e.m. p-values, Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pericyte-derived Angpt1 controls alveologenesis. a RT-qPCR analysis of Angpt1 and Tie2/Tek expression in freshly sorted lung GFP+, CD31+ or EpCAM+ cells from P7 Pdgfrb(BAC)-CreERT2 R26-mT/mG mice. Data represents mean ± s.e.m. (n = 4 mice). b High magnification images of P10 Angpt1GFP lungs stained for GFP (green), PDGFR beta (red), and PDGFR alpha (blue). Arrows indicate GFP and PDGFR beta double positive pericytes. Scale bar, 15 µm. c RT-qPCR analysis of Angpt1 expression in freshly sorted PDGFR beta + cells from P7 Yap1,Wwtr1iPCKO and control lungs. Data represents mean ± s.e.m. (n = 4 mice, two-tailed unpaired t-test). dAngpt1 expression in cultured Verteporfin (VP)-treated (48 h) and control pericytes. Data represents mean ± s.e.m. (n = 4, Welch’s t-test). e Expression of the indicated transcripts in freshly sorted CD31+ cells from P7 Yap1,Wwtr1iPCKO and control lungs. Data represents mean ± s.e.m. (n = 4 mice, NS not significant, two-tailed unpaired t-test). f–h Western blot analysis of Angpt1 protein (f; n = 2 controls and 4 mutant mice) and of total and phospho-Tie2 (pTie2) in P12 Yap1,Wwtr1iPCKO and control total lung lysates (g, n = 3 controls and 5 mutants). Molecular weight marker (kDa) is indicated. Relative quantification of signals is shown in h. Two-tailed unpaired t-test. i Scheme showing the time points of tamoxifen administration and analysis for Angpt1iPCKO mice. j, k 3D reconstruction confocal images of P12 Angpt1iPCKO and littermate control lungs stained for AQP5 (green), PDGFR beta (red), and PECAM1 (blue). Panels in k show higher magnification of PECAM1 staining. Scale bar, 50 µm (j) and 30 µm (k). l Quantitation of airspace volume in P12 Angpt1iPCKO and littermate control lung sections with 3D reconstruction surface images. Data represents mean ± s.e.m. (n = 4 mice; p < 0.0001, two-tailed unpaired t-test). m 3D reconstruction confocal images of P12 Angpt1iPCKO and littermate control lungs stained for alpha SMA (red) and tropoelastin (blue). Scale bar, 50 µm. n Quantitation of staining intensity for alpha SMA or tropoelastin shown in m. Intensity was normalized to the size of the parenchymal region. Data represents mean ± s.e.m. (n = 4 mice; NS not significant, two-tailed unpaired t-test). o Schematic summary of findings. Pulmonary pericytes regulate ECs and alveolar epithelial cells via angiocrine factors such as angiopoietin-1 and HGF. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29934496), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pericytes express functional Tie2.(a) Microarray-based expression screening of brain pericytes (BP), placenta pericytes (PP), pancreas pericytes (PA), lung pericytes (LP), muscle pericytes (MP) and human umbilical vein endothelial cells (HUVEC [HU]). (b) Semi-quantitative PCR of TEK (Tie2), endothelial marker genes (PECAM1, CDH5) and pericyte marker genes (CSPG4, CD248) in HUVEC and BP; house keeping gene: GAPDH. (c) Representative images showing expression of Tie2 in HUVEC and BP. (d) Histogram of membrane-bound Tie2 expression in HUVEC and BP compared to isotype control measured by flow cytometry. (e,g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells. (f,h) Quantification of the ratio of phosphorylated Tie2 (pTie2) relative to total Tie2 protein in BP (f) and HUVEC (h) normalized to us control (n=3). (i,k) Western blot analysis of AKT phosphorylation (Ser473) in control (shCtr) and Tie2-silenced (shTie2 I/shTie2 II) BP (i) and HUVEC (k) upon stimulation with rhAng1 or rhAng2. (j,l) Quantification of the ratio of pAKT to total AKT protein expression in BP (j) and HUVEC (l) normalized to unstimulated control (n=3). Representative western blot images are cropped versions and original images can be found in Supplementary Fig. 15. Scale bars: 20 μm (c). Data are shown as mean±s.d. Statistics were performed using Mann–Whitney U test (f,h) and one-way ANOVA (j,l). *P<0.05, **P<0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28719590), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Tie-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Tie‑2
  Ala23-Lys744
  Accession # CAA47857
• Specificity: Detects mouse and rat Tie-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) Tie‑2 is observed and less than 1% cross-reactivity with recombinant zebrafish Tie-2 and rhTie-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TEK
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-2 Antibody [Unconjugated]

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
2. Takakura, N. et al. (1998) Immunity 9:677.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tie-2 Antibody (AF762)(38)

Publications using AF762

• Cash, A;de Jager, C;Brickler, T;Soliman, E;Ladner, L;Kaloss, AM;Zhu, Y;Pridham, KJ;Mills, J;Ju, J;Basso, EKG;Chen, M;Johnson, Z;Sotiropoulos, Y;Wang, X;Xie, H;Matson, JB;Marvin, EA;Theus, MH; Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrity Proceedings of the National Academy of Sciences of the United States of America 2023-10-10 [PMID: 37796990] (IHC, Mouse)
• YW Koo, CS Lim, A Darai, J Lee, W Kim, I Han, GH Kim Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc Biomaterials research, 2023-03-29;27(1):26. 2023-03-29 [PMID: 36991502] (IHC, Rat)
• E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone Experimental & Molecular Medicine, 2023-02-24;55(2):470-484. 2023-02-24 [PMID: 36828931] (IHC, Mouse)
• K Shirakura, P Baluk, AF Nottebaum, U Ipe, KG Peters, DM McDonald, D Vestweber Shear stress control of vascular leaks and atheromas through Tie2 activation by VE-PTP sequestration Embo Molecular Medicine, 2023-02-06;0(0):e16128. 2023-02-06 [PMID: 36740996] (IHC, Mouse)
• A Lask, B Gutbier, O Kershaw, G Nouailles, AD Gruber, HC Müller-Red, S Chackowicz, DA Hamilton, P Van Slyke, M Witzenrath Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice Scientific Reports, 2022-09-15;12(1):15531. 2022-09-15 [PMID: 36109537] (Western Blot, Mouse)
• EA Kowalski, E Soliman, C Kelly, EK Gudenschwa, J Leonard, KJ Pridham, J Ju, AM Cash, A Hazy, C de Jager, AM Kaloss, H Ding, RD Hernandez, GM Coleman, X Wang, ML Olsen, AM Pickrell, MH Theus Monocyte pro-inflammatory phenotypic control by Ephrin type-A receptor 4 mediates neural tissue damage JCI Insight, 2022-08-08;0(0):. 2022-08-08 [PMID: 35737458] (IHC, Mouse)
• S Bhatia, D Nguyen, LB Darragh, B Van Court, J Sharma, MW Knitz, M Piper, S Bukkapatna, J Gadwa, TE Bickett, S Bhuvane, S Corbo, B Wu, Y Lee, M Fujita, M Joshi, LE Heasley, RL Ferris, O Rodriguez, C Albanese, M Kapoor, EB Pasquale, SD Karam EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment Nature Communications, 2022-06-20;13(1):3535. 2022-06-20 [PMID: 35725568] (IHC, Mouse)
• YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764. 2022-03-11 [PMID: 35360491] (IHC, Western Blot, Mouse)
• Jiali Zhu, Jinbao Li, Chun-Shiang Chung, Joanne L. Lomas-Neira, Alfred Ayala Patho-mechanisms for hemorrhage/sepsis-induced indirect acute respiratory distress syndrome (iARDS): A role for lung Tie1 and its regulation by neutrophils Shock 4/1/2022 [PMID: 34907117]
• Anoek L. I. van Leeuwen, Nicole A. M. Dekker, Paul Van Slyke, Esther de Groot, Marc G. Vervloet, Joris J. T. H. Roelofs et al. The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats Intensive Care Medicine Experimental 5/17/2021 [PMID: 33997943]
• B Lee, H Shin, JE Oh, J Park, M Park, SC Yang, JH Jun, SH Hong, H Song, HJ Lim An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1 Autophagy, 2020-06-17;0(0):1-18. 2020-06-17 [PMID: 32579471] (Western Blot, Mouse)
• M Park, SH Hong, SH Park, YS Kim, SC Yang, HR Kim, S Noh, S Na, HK Lee, HJ Lim, SW Lyu, H Song Perivascular Stem Cell-Derived Cyclophilin A Improves Uterine Environment with Asherman&#039;s Syndrome via HIF1alpha-Dependent Angiogenesis Mol. Ther., 2020-05-20;0(0):. 2020-05-20 [PMID: 32534604] (Western Blot, Mouse)
• A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency Int J Mol Sci, 2020-05-25;21(10):. 2020-05-25 [PMID: 32466219] (IHC, Transgenic Mouse)
• W Dong, R Li, H Yang, Y Lu, L Zhou, L Sun, D Wang, J Duan Mesenchymal-endothelial transition-derived cells as a potential new regulatory target for cardiac hypertrophy Sci Rep, 2020-04-20;10(1):6652. 2020-04-20 [PMID: 32313043] (IHC, Mouse)
• M Singhal, N Gengenbach, S La Porta, S Gehrs, J Shi, M Kamiyama, DM Bodenmille, A Fischl, B Schieb, E Besemfelde, S Chintharla, HG Augustin Preclinical validation of a novel metastasis-inhibiting Tie1 function-blocking antibody EMBO Mol Med, 2020-04-17;0(0):e11164. 4/17/2020 [PMID: 32302470]
• Z Li, EA Korhonen, A Merlini, J Strauss, E Wihuri, H Nurmi, S Antila, J Paech, U Deutsch, B Engelhardt, S Chintharla, GY Koh, A Flügel, K Alitalo Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS J. Clin. Invest., 2020-04-01;0(0):. 4/1/2020 [PMID: 32149735]
• H Chen, X Fu, J Jiang, S Han C16 Peptide Promotes Vascular Growth and Reduces Inflammation in a Neuromyelitis Optica Model Front Pharmacol, 2019-12-03;10(0):1373. 2019-12-03 [PMID: 31849648] (Western Blot, Rat)
• Xianghu Qu, Kate Violette, M. K. Sewell-Loftin, Jonathan Soslow, LeShana Saint-Jean, Robert B. Hinton et al. Loss of flow responsive Tie1 results in Impaired Aortic valve remodeling Developmental Biology 11/1/2019 [PMID: 31319059]
• Marie Besnier, Saran Shantikumar, Maryam Anwar, Parul Dixit, Aranzazu Chamorro-Jorganes, Walid Sweaad et al. miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia Molecular Therapy - Nucleic Acids 2019-09-06 [PMID: 31220779] (Western Blot, Mouse)
• Marie Besnier, Saran Shantikumar, Maryam Anwar, Parul Dixit, Aranzazu Chamorro-Jorganes, Walid Sweaad et al. miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia Molecular Therapy - Nucleic Acids 9/6/2019 [PMID: 31220779]
• AR Pedrosa, N Bodrug, J Gomez-Escu, EP Carter, LE Reynolds, PN Georgiou, I Fernandez, DM Lees, V Kostourou, AN Alexopoulo, S Batista, B Tavora, B Serrels, M Parsons, T Iskratsch, KM Hodivala-D Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861 Cancer Res., 2019-06-12;0(0):. 2019-06-12 [PMID: 31189647] (IHC, Western Blot, Mouse, Transgenic Mouse)
• J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration Sci Adv, 2019-02-13;5(2):eaau6732. 2019-02-13 [PMID: 30788433] (IHC-Fr, Mouse)
• Yi-Chun Tsai, Po-Lin Kuo, Wei-Wen Hung, Ling-Yu Wu, Ping-Hsun Wu, Wei-An Chang et al. Angpt2 Induces Mesangial Cell Apoptosis through the MicroRNA-33-5p-SOCS5 Loop in Diabetic Nephropathy Molecular Therapy - Nucleic Acids 12/7/2018 [PMID: 30414568]
• K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams Pulmonary pericytes regulate lung morphogenesis Nat Commun, 2018-06-22;9(1):2448. 2018-06-22 [PMID: 29934496] (Western Blot, Mouse)
• Boris Sabirzhanov, Alan I. Faden, Taryn Aubrecht, Rebecca Henry, Ethan Glaser, Bogdan A. Stoica MicroRNA-711–Induced Downregulation of Angiopoietin-1 Mediates Neuronal Cell Death Journal of Neurotrauma 10/15/2018 [PMID: 29774773]
• SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh Tie2 protects the vasculature against thrombus formation in systemic inflammation J. Clin. Invest., 2018-03-05;0(0):. 2018-03-05 [PMID: 29360642] (Western Blot, Human)
• J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm&#039;s canal integrity and induces glaucoma J. Clin. Invest., 2017-09-18;0(0):. 2017-09-18 [PMID: 28920924] (IHC, Mouse)
• R Gu, X Sun, Y Chi, Q Zhou, H Xiang, DB Bosco, X Lai, C Qin, KF So, Y Ren, XM Chen Integrin ?3/Akt signaling contributes to platelet-induced hemangioendothelioma growth Sci Rep, 2017-07-25;7(1):6455. 7/25/2017 [PMID: 28744026]
• N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling Circ. Res, 2017-02-08;0(0):. 2017-02-08 [PMID: 28179430] (IHC, Mouse)
• Man Chu, Taotao Li, Bin Shen, Xudong Cao, Haoyu Zhong, Luqing Zhang et al. Angiopoietin receptor Tie2 is required for vein specification and maintenance via regulating COUP-TFII eLife 12/22/2016 [PMID: 28005008]
• S. Egginton, A. Hussain, J. Hall-Jones, B. Chaudhry, F. Syeda, K. E. Glen Shear stress-induced angiogenesis in mouse muscle is independent of the vasodilator mechanism and quickly reversible Acta Physiol (Oxf) 11/1/2016 [PMID: 27261201]
• S. Egginton, A. Hussain, J. Hall-Jones, B. Chaudhry, F. Syeda, K. E. Glen Shear stress-induced angiogenesis in mouse muscle is independent of the vasodilator mechanism and quickly reversible Acta Physiol (Oxf) 2016-11-01 [PMID: 27261201] (Western Blot, Mouse)
• Zhou Y, Williams J, Smallwood P, Nathans J Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. PLoS ONE, 2015-12-02;10(12):e0143650. 2015-12-02 [PMID: 26630461] (IHC-Fr, Mouse)
• Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P Endothelial destabilization by angiopoietin-2 via integrin beta1 activation. Nat Commun, 2015-01-30;6(0):5962. 2015-01-30 [PMID: 25635707] (IHC, Mouse)
• Kosacka J, Nowicki M, Kloting N, Kern M, Stumvoll M, Bechmann I, Serke H, Bluher M COMP-angiopoietin-1 recovers molecular biomarkers of neuropathy and improves vascularisation in sciatic nerve of ob/ob mice. PLoS ONE, 2012-03-06;7(3):e32881. 2012-03-06 [PMID: 22412941] (Western Blot, Mouse)
• Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway. Biochem. Biophys. Res. Commun., 2008-11-24;378(2):296-301. 2008-11-24 [PMID: 19028450] (Western Blot, Mouse)
• Ohab JJ, Fleming S, Blesch A, Carmichael ST A neurovascular niche for neurogenesis after stroke. J. Neurosci., 2006-12-13;26(50):13007-16. 2006-12-13 [PMID: 17167090] (Neutralization, Mouse)
• Luo Y, Wen YJ, Ding ZY, Fu CH, Wu Y, Liu JY, Li Q, He QM, Zhao X, Jiang Y, Li J, Deng HX, Kang B, Mao YQ, Wei YQ Immunotherapy of tumors with protein vaccine based on chicken homologous Tie-2. Clin. Cancer Res., 2006-03-15;12(6):1813-9. 2006-03-15 [PMID: 16551866] (Western Blot, Chicken, Mouse)

Reviews for Tie-2 Antibody (AF762) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Hayk Simonyan ICC Mouse 06/08/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: 4940946 &#40;order number&#41;

reviewed by: Hayk Simonyan IHC Mouse 05/12/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Adult mouse brain tissue
• Species: Mouse

Bioinformatics

• Gene Symbol: TEK
• Uniprot:
  • CAA47857()