Tie-2 [p Tyr1102, p Tyr1100, p Tyr1100] Antibody [Unconjugated]

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line transfected with mouse Tie-2 untreated (-) or treated (+) with 600 ng/mL Recombinant Human Angiopoietin-1 (Catalog # 923-AN) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Phospho-Tie-2 (Y1102/Y1100) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3909) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Tie-2 (Y1102/1100) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Tie-2 phosphorylated at Y1102 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human/Mouse Phospho-Tie-2 (Y1102/Y1100) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3909) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Tie-2 [p Tyr1102, p Tyr1100, p Tyr1100] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human Tie-2 Y1102 site
• Modification: p Tyr1100, p Tyr1102, p Tyr1100
• Specificity: Detects human and mouse Tie-2 when phosphorylated at Y1102 and Y1100, respectively.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TEK
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-2 [p Tyr1102, p Tyr1100, p Tyr1100] Antibody [Unconjugated]

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

Tie-2 (also known as TEK) is an angiogenic receptor tyrosine kinase required for the later stage of blood vessel maturation. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation of human Tie-2 at Y1102 and mouse Tie-2 at Y1100 results in the activation of PI 3‑kinase (1, 2).

1. Kontos, C.D. et al. (1998) Mol. Cell Biol. 18:4131.
2. Jones, N. et al. (1999) J. Biol. Chem. 274:30896.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tie-2 Antibody (AF3909)(5)

Publications using AF3909

• Vidya P. Kumar, Sasha Stone, Shukla Biswas, Neel Sharma, Sanchita P. Ghosh Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury Frontiers in Pharmacology 2020-11-12 [PMID: 33343356]
• M Park, SH Hong, SH Park, YS Kim, SC Yang, HR Kim, S Noh, S Na, HK Lee, HJ Lim, SW Lyu, H Song Perivascular Stem Cell-Derived Cyclophilin A Improves Uterine Environment with Asherman's Syndrome via HIF1alpha-Dependent Angiogenesis Mol. Ther., 2020-05-20;0(0):. 2020-05-20 [PMID: 32534604] (Western Blot, Mouse)
• D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury Front Pharmacol, 2018-04-27;9(0):369. 2018-04-27 [PMID: 29755348] (IHC, Mouse)
• J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis Sci Rep, 2017-03-09;7(0):44113. 2017-03-09 [PMID: 28276491] (Western Blot, Human)
• van der Heijden M, van Nieuw Amerongen GP, van Bezu J, Paul MA, Groeneveld AB, van Hinsbergh VW Opposing effects of the angiopoietins on the thrombin-induced permeability of human pulmonary microvascular endothelial cells. PLoS ONE, 2011-08-15;6(8):e23448. 2011-08-15 [PMID: 21858121] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TEK