Tie-2 [p Tyr992] Antibody [Unconjugated]

Images

 
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line transfected with mouse Tie-2 and untreated (-) or treated (+) with 600 ng/mL Recombinant Human Angiopoietin‑1 (Catalog # 923-AN) for ...read more
HUVEC human umbilical vein endothelial cells were unstimulated (light orange filled histogram) or treated with 100 μM pervanadate for 15 minutes (dark orange filled histogram), then stained with Human/Mouse ...read more
Tie‑2 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Rabbit Anti-Human/Mouse Phospho-Tie‑2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Tie-2 [p Tyr992] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human Tie-2 Y992 site
Modification
p Tyr992
Specificity
Detects human and mouse Tie-2 when phosphorylated at Y992 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TEK
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry 1-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
AF2720 in the following applications:

Publications
Read Publications using
AF2720 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-2 [p Tyr992] Antibody [Unconjugated]

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

Tie-2 (also known as TEK) is an angiogenic receptor tyrosine kinase required for the later stage of blood vessel maturation. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Y992 is located on the putative activation loop of Tie-2 and is a major autophosphorylation site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

923-AN/CF
Species: Hu
Applications: BA
DANG20
Species: Hu
Applications: ELISA
DVE00
Species: Hu
Applications: ELISA
AF321
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
AF644
Species: Mu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
AF3628
Species: Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, WB
AF619
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
DAN00
Species: Hu
Applications: ELISA
NB110-41083
Species: Hu
Applications: ChIP, ELISA, ICC/IF, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
210-TA
Species: Hu
Applications: BA
AF619
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
7268-CT
Species: Hu
Applications: BA
AF1002
Species: Mu
Applications: IHC, Simple Western, WB
NB300-500
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB

Publications for Tie-2 Antibody (AF2720)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


Filter By Application
ICC
(2)
IHC
(2)
Western Blot
(17)
All Applications
Filter By Species
Human
(11)
Mouse
(10)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF2720 Applications Species
A Lask, B Gutbier, O Kershaw, G Nouailles, AD Gruber, HC Müller-Red, S Chackowicz, DA Hamilton, P Van Slyke, M Witzenrath Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice Scientific Reports, 2022;12(1):15531. 2022 [PMID: 36109537] (Western Blot, Mouse) Western Blot Mouse
YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022;16(0):848764. 2022 [PMID: 35360491] (Western Blot, Mouse) Western Blot Mouse
I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk Nature Neuroscience, 2021;0(0):. 2021 [PMID: 33510480] (IHC, Mouse) IHC Mouse
ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils Sulfated glycans engage the Ang-Tie pathway to regulate vascular development Nat Chem Biol, 2020;0(0):. 2020 [PMID: 33020664] (Western Blot, Human) Western Blot Human
J Gu, Y Zhang, Z Han, L Gao, J Cui, Y Sun, Y Niu, B You, CP Huang, C Chang, X Wang, S Yeh Targeting the ER&beta/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC Cell Death Dis, 2020;11(5):367. 2020 [PMID: 32409702] (Western Blot, Human) Western Blot Human
M Singhal, N Gengenbach, S La Porta, S Gehrs, J Shi, M Kamiyama, DM Bodenmille, A Fischl, B Schieb, E Besemfelde, S Chintharla, HG Augustin Preclinical validation of a novel metastasis-inhibiting Tie1 function-blocking antibody EMBO Mol Med, 2020;0(0):e11164. 2020 [PMID: 32302470] (Western Blot, Mouse) Western Blot Mouse
K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams Pulmonary pericytes regulate lung morphogenesis Nat Commun, 2018;9(1):2448. 2018 [PMID: 29934496] (Western Blot, Mouse) Western Blot Mouse
Y Lu, PWL Tai, J Ai, DJ Gessler, Q Su, X Yao, Q Zheng, PD Zamore, X Xu, G Gao Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs Mol Ther Nucleic Acids, 2018;10(0):349-360. 2018 [PMID: 29499946] (Western Blot, Mouse) Western Blot Mouse
D Ko, S Kim Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF Oncotarget, 2018;9(1):726-742. 2018 [PMID: 29416649] (Western Blot, Human) Western Blot Human
SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh Tie2 protects the vasculature against thrombus formation in systemic inflammation J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29360642] (Western Blot, Human) Western Blot Human
Show All 21 Publications.

Review for Tie-2 Antibody (AF2720) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2720:
Filter by Applications
Immunofluorescence in mouse brain tissue
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Other Tie-2 AF2720
Enlarge
3
reviewed by:
Bhavin Shah
Immunofluorescence in mouse brain tissue Mouse 10/02/2020
View

Summary

ApplicationOther
Sample TestedCerebellum tissue
SpeciesMouse
LotVRQ0314031

Comments

CommentsPostnatal mouse cerebellum at P14 was used to determine the levels of phospho-Tie2 in the blood vessels. The blocking buffer and the antibody buffers were prepared in 0.2% gelatin in PBST (In primary and secondary solutions were used 0.025% of Saponin was added). The sections were antigen retrieved by boiling in a microwave for 5 min using 10mM Sodium citrate (pH6).

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Tie-2 Antibody (AF2720) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Tie-2 Products

Bioinformatics Tool for Tie-2 Antibody (AF2720)

Discover related pathways, diseases and genes to Tie-2 Antibody (AF2720). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Tie-2 Antibody (AF2720)

Discover more about diseases related to Tie-2 Antibody (AF2720).
 

Pathways for Tie-2 Antibody (AF2720)

View related products by pathway.

PTMs for Tie-2 Antibody (AF2720)

Learn more about PTMs related to Tie-2 Antibody (AF2720).

Blogs on Tie-2.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Bhavin Shah
10/02/2020
Application: Immunofluorescence in mouse brain tissue
Species: Mouse

Bioinformatics

Gene Symbol TEK