TGF-beta 1 Antibody [Biotin]

• Reactivity: All-Multi
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Biotin
• Concentration: LYOPH

TGF-beta 1 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 (R&D Systems, Catalog # 240-B)
• Specificity: Detects human TGF-beta 1 in ELISAs and Western blots. In sandwich immunoassays, when used in combination with the rhTGF‑ beta sRII/Fc chimera (Catalog # 341-BR), approximately 15% cross-reactivity with recombinant human (rh) Latent TGF‑ beta 1 is observed, 1% cross-reactivity with rhTGF-beta 1.2 is observed, and 3% cross-reactivity with recombinant amphibian (ra) TGF-beta 5 is observed. When used in combination with the monoclonal capture antibody (Catalog # MAB2402), approximately 5% cross‑reactivity with TGF-beta 1.2 is observed and 1% cross-reactivity with rhTGF-beta 2, recombinant chicken TGF-beta 3, and raTGF-beta 5 is observed.
• Source: N/A
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: TGFB1
• Purity Statement: Antigen Affinity-purified from egg yolks

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Recombinant Human TGF-beta RII Fc Chimera (Catalog # 341-BR)
  ELISA Capture:TGF-beta 1, 2, 3 Antibody (Catalog # MAB1835)
  ELISA Capture:TGF-beta 1 Antibody (Catalog # MAB240)ELISA Detection:TGF-beta 1 Biotinylated Antibody (Catalog # BAF240)(Catalog # BAM9731)
  Standard: Recombinant Human TGF-beta 1 (Catalog # 240-B)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 1 Antibody [Biotin]

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

TGF- beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1 - 7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1 - 4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8 - 10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity (1). TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

 

1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
5. Lin, J.S. et al. (2006) Reproduction 132:179.
6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
8. Derynck, R. et al. (1985) Nature 316:701.
9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 1 Antibody (BAF240)(43)

Publications using BAF240

• SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs Nature Immunology, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456734] (ELISA Detection, Human)
• AH Kadam, K Kandasamy, T Buss, B Cederstrom, C Yang, S Narayanapi, J Rodriguez, MD Levin, J Koziol, B Olenyuk, Z Borok, A Chrastina, JE Schnitzer Targeting caveolae to pump bispecific antibody to TGF-beta into diseased lungs enables ultra-low dose therapeutic efficacy PLoS ONE, 2022-11-22;17(11):e0276462. 2022-11-22 [PMID: 36413536] (Neutralization, Western Blot, Rat)
• Shimaa A. Amer, Ahmed A. A. Abdel-Wareth, Ahmed Gouda, Gehan K. Saleh, Arwa H. Nassar, Wafaa R. I. A. Sherief et al. Impact of Dietary Lavender Essential Oil on the Growth and Fatty Acid Profile of Breast Muscles, Antioxidant Activity, and Inflammatory Responses in Broiler Chickens Antioxidants (Basel) 9/13/2022 [PMID: 36139872]
• Fariba Poosti, Mohammad Ayodhia Soebadi, Helena Crijns, Alexandra De Zutter, Mieke Metzemaekers, Nele Berghmans et al. Inhibition of renal fibrosis with a human CXCL9‐derived glycosaminoglycan‐binding peptide Clinical & Translational Immunology 2/2/2022 [PMID: 35140938]
• S Li, M Liu, MH Do, C Chou, EG Stamatiade, BG Nixon, W Shi, X Zhang, P Li, S Gao, KJ Capistrano, H Xu, NV Cheung, MO Li Cancer immunotherapy via targeted TGF-&beta signalling blockade in TH cells Nature, 2020-10-21;0(0):. 2020-10-21 [PMID: 33087933] (Mouse)
• N Kaushansky, E Bakos, S Becker-Her, I Shachar, A Ben-Nun Circulating Picomolar Levels of CCL2 Downregulate Ongoing Chronic Experimental Autoimmune Encephalomyelitis by Induction of Regulatory Mechanisms J. Immunol., 2019-09-04;0(0):. 2019-09-04 [PMID: 31484731] (ELISA Detection, Mouse)
• H Yu, S Ma, L Sun, J Gao, C Zhao TGF??1 upregulates the expression of lncRNA?ATB to promote atherosclerosis Mol Med Rep, 2019-03-29;19(5):4222-4228. 2019-03-29 [PMID: 30942415] (ELISA Development (Detection), Human)
• James Warren, Michelle Im, Angela Ballesteros, Cam Ha, Tom Moore, Fanny Lambert et al. Activation of latent transforming growth factor-beta 1, a conserved function for pregnancy-specific beta 1-glycoproteins MHR: Basic science of reproductive medicine 12/1/2018 [PMID: 30371828]
• N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype Anticancer Res., 2018-04-01;38(4):2007-2014. 4/1/2018 [PMID: 29599317]
• R Ravi, KA Noonan, V Pham, R Bedi, A Zhavoronko, IV Ozerov, E Makarev, A V Artemov, PT Wysocki, R Mehra, S Nimmagadda, L Marchionni, D Sidransky, IM Borrello, E Izumchenko, A Bedi Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGF? enhance the efficacy of cancer immunotherapy Nat Commun, 2018-02-21;9(1):741. 2018-02-21 [PMID: 29467463] (ELISA Development (Detection))
• TK Ukah, AN Cattin-Roy, W Chen, MM Miller, S Barik, H Zaghouani On the Role IL-4/IL-13 Heteroreceptor Plays in Regulation of Type 1 Diabetes J. Immunol., 2017-06-23;0(0):. 6/23/2017 [PMID: 28646042]
• Ryan J. Coram, Samantha J. Stillwagon, Anuradha Guggilam, Michael W. Jenkins, Maurice S. Swanson, Andrea N. Ladd Muscleblind-like 1 is required for normal heart valve development in vivo BMC Developmental Biology 12/1/2015 [PMID: 26472242]
• Kittikhun Wangkanont, Katrina T. Forest, Laura L. Kiessling The non-detergent sulfobetaine-201 acts as a pharmacological chaperone to promote folding and crystallization of the type II TGF-beta receptor extracellular domain Protein Expression and Purification 11/1/2015 [PMID: 26073093]
• Kaushansky N, Eisenstein M, Boura-Halfon S, Hansen B, Nielsen C, Milo R, Zeilig G, Lassmann H, Altmann D, Ben-Nun A Role of a Novel Human Leukocyte Antigen-DQA1*01:02;DRB1*15:01 Mixed Isotype Heterodimer in the Pathogenesis of "Humanized" Multiple Sclerosis-like Disease. J Biol Chem, 2015-04-24;290(24):15260-78. 2015-04-24 [PMID: 25911099] (ELISA Development (Detection), Mouse)
• Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo. Nat Commun, 2015-02-19;6(0):6353. 2015-02-19 [PMID: 25695215] (Flow Cytometry, Mouse)
• Ballesteros A, Mentink-Kane M, Warren J, Kaplan G, Dveksler G Induction and activation of latent transforming growth factor-beta1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). J Biol Chem, 2014-12-29;290(7):4422-31. 2014-12-29 [PMID: 25548275] (ELISA Development (Detection), Mouse)
• Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. J Immunol, 2013-12-11;192(2):804-16. 2013-12-11 [PMID: 24337375] (Flow Cytometry, Mouse)
• David Eastwood, Chris Bird, Paula Dilger, Jason Hockley, Lucy Findlay, Stephen Poole et al. Severity of the TGN1412 trial disaster cytokine storm correlated with IL-2 release British Journal of Clinical Pharmacology 8/1/2013 [PMID: 23701319]
• Arthur M. Blum, Long Hang, Tommy Setiawan, Joseph P. Urban, Korynn M. Stoyanoff, John Leung et al. Heligmosomoides polygyrus bakeri Induces Tolerogenic Dendritic Cells that Block Colitis and Prevent Antigen-Specific Gut T Cell Responses The Journal of Immunology 9/1/2012 [PMID: 22844110]
• Abdoreza Davoodi-Semiromi, Clive H. Wasserfall, Chang Qing Xia, Rhonda M. Cooper-DeHoff, Martin Wabitsch, Michael Clare-Salzler et al. The Tyrphostin Agent AG490 Prevents and Reverses Type 1 Diabetes in NOD Mice PLoS ONE 5/14/2012 [PMID: 22615750]
• Nathali Kaushansky, Daniel M Altmann, Chella S David, Hans Lassmann, Avraham Ben-Nun DQB1*0602 rather than DRB1*1501 confers susceptibility to multiple sclerosis-like disease induced by proteolipid protein (PLP) Journal of Neuroinflammation 12/1/2012 [PMID: 22316121]
• Nathali Kaushansky, Nicole Kerlero de Rosbo, Rina Zilkha-Falb, Reut Yosef-Hemo, Lydia Cohen, Avraham Ben-Nun ‘Multi-Epitope-Targeted’ Immune-Specific Therapy for a Multiple Sclerosis-Like Disease via Engineered Multi-Epitope Protein Is Superior to Peptides PLoS ONE 11/29/2011 [PMID: 22140475]
• Moses K. Donkor, Abira Sarkar, Peter A. Savage, Ruth A. Franklin, Linda K. Johnson, Achim A. Jungbluth et al. T Cell Surveillance of Oncogene-Induced Prostate Cancer Is Impeded by T Cell-Derived TGF-beta 1 Cytokine Immunity 7/22/2011 [PMID: 21757379]
• Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia, 2011-07-09;54(10):2713-23. 2011-07-09 [PMID: 21744073] (ELISA Development, Human)
• Takatoku Oida, Howard L. Weiner Murine CD4 T Cells Produce a New Form of TGF-beta as Measured by a Newly Developed TGF-beta Bioassay PLoS ONE 4/11/2011 [PMID: 21494571]
• Ilona Gutcher, Moses K. Donkor, Qian Ma, Alexander Y. Rudensky, Richard A. Flavell, Ming O. Li Autocrine Transforming Growth Factor-beta 1 Promotes In Vivo Th17 Cell Differentiation Immunity 3/25/2011 [PMID: 21435587]
• Takatoku Oida, Howard L. Weiner Depletion of TGF-beta from fetal bovine serum Journal of Immunological Methods 10/31/2010 [PMID: 20837018]
• Long Hang, Tommy Setiawan, Arthur M. Blum, Joseph Urban, Korynn Stoyanoff, Seiji Arihiro et al. Heligmosomoides polygyrus Infection Can Inhibit Colitis through Direct Interaction with Innate Immunity The Journal of Immunology 9/15/2010 [PMID: 20702728]
• Smriti Mondal, Pradyot Bhattacharya, Mehebubar Rahaman, Nahid Ali, Rama Prosad Goswami A Curative Immune Profile One Week after Treatment of Indian Kala-Azar Patients Predicts Success with a Short-Course Liposomal Amphotericin B Therapy PLoS Neglected Tropical Diseases 7/27/2010 [PMID: 20668544]
• Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al. Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation Biology of Blood and Marrow Transplantation 4/1/2011 [PMID: 20457265]
• Nor E. Sounni, Kerstin Dehne, Leon van Kempen, Mikala Egeblad, Nesrine I. Affara, Ileana Cuevas et al. Stromal regulation of vessel stability by MMP14 and TGFbeta Disease Models & Mechanisms 4/28/2010 [PMID: 20223936]
• Nor E. Sounni, Kerstin Dehne, Leon van Kempen, Mikala Egeblad, Nesrine I. Affara, Ileana Cuevas et al. Stromal regulation of vessel stability by MMP14 and TGFbeta Disease Models & Mechanisms 2010-04-28 [PMID: 20223936] (Immunoprecipitation, ELISA Detection, Mouse)
• Danielle M. Tartar, Amie M. VanMorlan, Xiaoxiao Wan, F. Betul Guloglu, Renu Jain, Cara L. Haymaker et al. FoxP3+RORgammat+ T helper intermediates display suppressive function against autoimmune diabetes The Journal of Immunology 4/1/2010 [PMID: 20181889]
• Danielle M. Tartar, Amie M. VanMorlan, Xiaoxiao Wan, F. Betul Guloglu, Renu Jain, Cara L. Haymaker et al. FoxP3+RORgammat+ T helper intermediates display suppressive function against autoimmune diabetes The Journal of Immunology 2010-04-01 [PMID: 20181889] (Flow Cytometry, Mouse)
• Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M Activation of Toll-like receptor 3 augments myofibroblast differentiation. Am. J. Respir. Cell Mol. Biol., 2008-11-06;40(6):654-62. 2008-11-06 [PMID: 18988918] (ELISA Development, Human)
• Saurer L, McCullough KC, Summerfield A In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid. J. Immunol., 2007-09-15;179(6):3504-14. 2007-09-15 [PMID: 17785784] (ELISA Development, Porcine)
• Barboza L, Salmen S, Goncalves L, Colmenares M, Peterson D, Montes H, Cartagirone R, Gutierrez Mdel C, Berrueta L Antigen-induced regulatory T cells in HBV chronically infected patients. Virology, 2007-07-23;368(1):41-9. 2007-07-23 [PMID: 17643462] (Flow Cytometry, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)
• Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression. Immunol. Lett., 2006-03-03;105(2):159-66. 2006-03-03 [PMID: 16564576] (ELISA Development, Human)
• Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5. 2004-12-01 [PMID: 15585763] (ELISA Development, Human)
• Chen , WanJun, Jin , Wenwen, Hardegen , Neil, Lei , Ke-Jian, Li , Li, Marinos , Nancy, McGrady , George, Wahl , Sharon M Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3. J Exp Med, 2003-12-15;198(12):1875-86. 2003-12-15 [PMID: 14676299] (Flow Cytometry, Mouse)
• Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56. 2002-05-01 [PMID: 11943670] (ELISA Development, Human)
• Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4. J. Immunol., 2000-05-15;164(10):5337-43. 2000-05-15 [PMID: 10799896] (ELISA Development, Primate - Papio anubis (Olive Baboon))

Bioinformatics

• Gene Symbol: TGFB1