TGF-beta 1 Antibody (7F6)

Images

 
Western Blot: TGF-beta 1 Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human TGF beta 1 recombinant protein. (Expected MW is 41 kDa)
Immunohistochemistry-Paraffin: TGF-beta 1 Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using TGF beta 1 mouse mAb with DAB staining.
Flow Cytometry: TGF-beta 1 Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using TGF beta 1 mouse mAb (green) and negative control (red).
Western Blot: TGF-beta 1 Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.
Immunohistochemistry-Paraffin: TGF-beta 1 Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.
Immunohistochemistry-Paraffin: TGF-beta 1 Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using TGF beta 1 mouse mAb with DAB staining.
ELISA: TGF-beta 1 Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC-P
Clone
7F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

TGF-beta 1 Antibody (7F6) Summary

Immunogen
Purified recombinant fragment of human TGF beta 1 (amino acids 62-197) expressed in E. coli. [UniProt# P01137]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TGFB1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This TGF beta 1 (7F6) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-22114 in the following applications:

Publications
Read Publications using
NBP2-22114 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26851347)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for TGF-beta 1 Antibody (7F6)

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta 1 Antibody (NBP2-22114)(4)

Reviews for TGF-beta 1 Antibody (NBP2-22114) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: IHC-P.

Reviews using NBP2-22114:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 10/28/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney
SpeciesHuman
Lot130520
Immunohistochemistry-Paraffin TGF-beta 1 NBP2-22114
Enlarge
reviewed by:
Hayley Derricott
IHC-P Human 04/04/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lot130102

Blocking

Blocking DetailsNon-immune block (swine serum, human serum, TBS-T 0.1%), 30min at RT

Primary Anitbody

Dilution Ratio10µg/mL (1:100) in non-immune block incubated at 4 degrees overnight

Secondary Antibody

Secondary DescriptionBiotinylated goat anti-mouse IgG

Details

Detection NotesDAB, 9 minutes. Negative control mouse IgG matched concentration to primary.
Fixation DetailsFormalin-fixed, wax-embedded, 10min heat-mediated citrate buffer Ag retrieval (20min cool), 10min peroxidase block
Wash Description1 TBS wash 5min, 2 TBS-T 0.6% washes 5min, 1 TBS wash 5min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TGF-beta 1 Antibody (NBP2-22114) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TGF-beta 1 Antibody (NBP2-22114)

Discover related pathways, diseases and genes to TGF-beta 1 Antibody (NBP2-22114). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TGF-beta 1.

TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
10/28/2015
Application: IHC-P
Species: Human

Hayley Derricott
04/04/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol TGFB1
Entrez
Uniprot